主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

北京中医药大学学报 ›› 2014, Vol. 37 ›› Issue (1): 33-38.doi: 10.3969/j.issn.1006-2157.2014.01.008

• 科技之窗 • 上一篇    下一篇

接近临床剂量的甘遂半夏汤加减甘遂甘草反药组合对腹水大鼠肝肾功能的影响

柳海艳1, 钟赣生1, 刘佳1, 王茜2, 李怡文1, 刘云翔1, 高源1, 欧丽娜1   

  1. 1 北京中医药大学基础医学院 北京 100029;
    2 河北中医学院
  • 收稿日期:2013-06-19 出版日期:2014-01-30 发布日期:2014-01-30
  • 通讯作者: 钟赣生,男,教授,博士生导师,研究方向:中药配伍禁忌本质的研究, E-mail:zhonggansheng@sohu.com
  • 作者简介:柳海艳,女,在读博士生
  • 基金资助:
    国家重点基础研究发展计划(973计划)资助项目(No.2011CB505300,No.2011CB505306)

Influences of antagonism combinations of Gancao (Radix Glycyrrhizae) and Gansui (Radix Euphordiae Kansui) in modified Gansui Banxia Tang on hepatic and renal functions in rats with ascites

LIU Hai-yan1, ZHONG Gan-sheng1, LIU Jia1, WANG Xi2, LI Yi-wen1, LIU Yun-xiang1, GAO Yuan1, OU Li-na1   

  1. 1 School of Preclinical Medicine, Beijing University of Chinese Medicine, Beijing 100029;
    2 Hebei University of Traditional Chinese Medicine
  • Received:2013-06-19 Online:2014-01-30 Published:2014-01-30

摘要: 目的 通过甘遂半夏汤中甘遂甘草反药组合加减应用对癌性腹水模型大鼠肝肾功能的影响,探讨药效或毒性反应是否与反药组合相关。方法 将Wistar大鼠按体重随机分为空白组、模型组、呋塞米组、甘遂半夏汤全方组、甘遂半夏汤去醋甘遂组、甘遂半夏汤去炙甘草组,甘遂半夏汤去炙甘草醋甘遂组,各给药组灌胃给药,空白组和模型组灌胃蒸馏水,灌胃体积均为10 mL/kg。给药9 d后,所有大鼠禁食不禁水12 h,取血,离心检测肝肾功能相关指标。结果 与模型组比较,甘遂半夏汤全方组血中AST/ALT降低(P<0.01),碱性磷酸酶(ALP)升高(P<0.05)、总蛋白(TP)含量升高(P<0.05)、白蛋白(ALB)含量与A/G比值升高且具有统计学差异(P<0.05或P<0.01);血中尿素氮(BUN)的含量降低(P<0.05)、肌酐(CRE)的含量降低且具有统计学差异(P<0.05)。与甘遂半夏汤全方组比较,甘遂半夏汤全方去炙甘草组、去醋甘遂组和去草遂组AST/ALT比值升高且具有统计学差异(P<0.05或P<0.01),而甘遂半夏汤全方去炙甘草组、去醋甘遂组和去草遂组血中ALB含量与A/G比值均较甘遂半夏汤全方组降低且具有统计学差异(P<0.05或P<0.01)。结论 甘遂半夏汤全方组和甘遂半夏汤全方去掉其中一味反药或两味反药组在肝肾功能方面均未显示出明显毒性,且含甘遂甘草反药组合的甘遂半夏汤全方对于保护肝肾功能方面的药效优于全方去掉其中的一味反药或两味反药,反药组合在全方中有可能有增强药效的作用。

关键词: 临床应用剂量, 甘遂半夏汤, 甘遂甘草反药组合, 腹水, 肝肾功能, 大鼠

Abstract: Objective To discuss whether the efficacy or tocicity being correlated to the antagonism combinations of Gancao (Radix Glycyrrhizae) and Gansui (Radix Euphordiae Kansui) in modified Gansui Banxia Tang through observing the influences of the antagonism combinations on hepatic and renal functions in rats with malignant ascites. Methods Wistar rats were divided, according to weight, into blank group, model group, furosemide group, whole formula group of Gansui Banxia Tang (whole formula group), non-Gansui group, non-Gancao group and non-Gansui non-Gancao group. Besides of normal feeding, furosemide group, whole formula group, non-Gansui group, non-Gancao group and non-Gansui non-Gancao group were intragastrically given corresponding medicinal (10 mL/kg), and blank group and model group were orally given distilled water (10 mL/kg). After 9 d, all rats were fasted except of water for 12 h. The blood samples were collected and centrifugated for detecting relative indexes of hepatic and renal functions. Results Compared with model group, AST/ALT decreased (P<0.01), ALP increased (P<0.05), total protein (TP) increased (P<0.05), ratio of albumin (ALB) to A/G increased (P<0.05 or P<0.01), blood urea nitrogen (BUN) decreased (P<0.05) and creatinine (CRE) decreased (P<0.05) in whole formula group. Compared with whole formula group, AST/ALT increased (P<0.05 or P<0.01) and ratio of ALB to A/G decreased (P<0.05 or P<0.01) in non-Gansui group, non-Gancao group and non-Gansui non-Gancao group. Conclusion In whole formula group and the groups without one or two antagonism medicinal, there is no obvious toxicity observed for hepatic and renal functions, while the medicinal efficacy is higher in whole formula group. Antagonism combinations may have effect of improving medicinal efficacy.

Key words: dose of clinical application, Gansui Banxia Tang, antagonism combinations of Gancao (Radix Glycyrrhizae) and Gansui (Radix Euphordiae Kansui), ascites, hepatic and renal functions, rats

中图分类号: 

  • R285.5