主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

北京中医药大学学报 ›› 2014, Vol. 37 ›› Issue (4): 280-284.doi: 10.3969/j.issn.1006-2157.2014.01.016

• 针灸 • 上一篇    下一篇

本经取穴调控偏头痛患者FAH基因、PTGS2基因的机制研究

黄银兰1, 万明雨2, 梁希森1, 梁繁荣3   

  1. 1 宁夏医科大学 宁夏 750003;
    2 北京中医药大学东方学院;
    3 成都中医药大学
  • 收稿日期:2013-06-24 出版日期:2014-04-30 发布日期:2014-04-30
  • 作者简介:黄银兰,女,博士,副教授,硕士生导师
  • 基金资助:
    国家自然科学基金资助项目(No.30960485); 国家重点基础研究发展计划(973计划)资助项目(No.2012CB518500)

Regulation of FAH gene and PTGS2 gene by acupoint selection on the same meridian for controlling migraine

HUANG Yin-lan1, WAN Ming-yu2, LIANG Xi-sen1, LIANG Fan-rong3   

  1. 1 Ningxia Medicine University, Ningxia 750003;
    2 Dongfang College, Beijing University of Chinese Medicine;
    3 Chengdu University of Traditional Chinese Medicine
  • Received:2013-06-24 Online:2014-04-30 Published:2014-04-30

摘要: 目的 观察本经取穴调控偏头痛患者延胡索二酰乙酰水解酶基因(FAH)、前列腺素内过氧化物含酶基因(PTGS2)差异表达。方法 20例偏头痛患者经辨证分型确诊为肝阳上亢型偏头痛,随机分为经穴组和非经非穴组,每组各10例,经穴组针刺患侧角孙、外关、阳陵泉、丘墟4穴;非经非穴组针刺患侧4个非经非穴点,每日1次,共治疗10次。提取全部患者治疗前后的静脉外周血,通过基因芯片技术,获得2组患者治疗前后的基因表达谱,并进行Gene Ontology(GO)分析,并且对2组治疗前后进行VAS量表评价。结果 FAH基因、PTGS2基因在经穴组各样本中表达显著降低,通过GO分析,FAH基因P<0.05,PTGS2基因P<0.05,这2个基因在非经非穴组各样本中未见表达。VAS量表评价2组治疗前无差异(P>0.05)。经穴组治疗前后有差异(P<0.05);经穴组治疗后与随访后有差异(P<0.05)。非经非穴组治疗前后有差异(P<0.05);非经非穴组治疗后与随访后无差异(P>0.05)。2组治疗前后的差值d相比较有差异(P<0.05),经穴组比非经非穴组即时疗效、远期疗效均显著。结论 本经取穴对肝阳上亢型无先兆偏头痛的效应机制之一可能是通过对FAH基因、PTGS2基因的调控实现的。

关键词: 延胡索二酰乙酰水解酶基因, 前列腺素内过氧化物含酶基因, 本经取穴, 无先兆偏头痛, 经穴效应

Abstract: Objective To observe the difference in the expressions of FAH gene and PTGS2 gene regulated by acupoint selection on the same meridian in patients with migraine. Methods The patients (n=20) were diagnosed as migraine (type of hyperactivity of liver-yang), and then divided into acupoint group and non-acupoint group (each n=10). The acupoint group was given needling in Jiaosun (TE20), Waiguan (TE15), Yanglingquan (GB34) and Qiuxu (GB40), and non-acupoint group was given needling in 4 non-acupoints once a day for 10 times. The samples of peripheral vein blood were collected from all patients before and after treatment, and genetic expression profiles were obtained by using gene chip technology. After Gene Ontology (GO) analysis, the profiles were reviewed with Visual Analogue Scale (VAS) before and after treatment in two groups. Results The expressions of FAH gene and PTGS2 gene decreased significantly in all samples in acupoint group (FAH: P<0.05, PTGS2: P<0.05 after GO analysis), while there were no expressions of these two genes observed in non-acupoint group. The results of VAS review showed that there was no difference between two groups before treatment (P>0.05). In acupoint group, there was difference before and after treatment (P<0.05), and there was difference after treatment and after follow-up (P<0.05). In non-acupoint group, there was difference before and after treatment (P<0.05), and there was no difference after treatment and after follow-up (P>0.05). The comparison in D-value in two groups before and after treatment showed difference (P<0.05). The immediate curative effect and long-term curative effect were more significant in acupoint group than those in non-acupoint group. Conclusion Acupoint selection on the same meridian has curative effect on migraine without aura (type of hyperactivity of liver-yang) and one of mechanisms may related to regulating FAH gene and PTGS2 gene.

Key words: FAH gene, PTGS2 gene, acupoint selection on the same meridian, migraine without aura, meridian acupoint effect

中图分类号: 

  • R245