主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

北京中医药大学学报 ›› 2014, Vol. 37 ›› Issue (3): 190-194.doi: 10.3969/j.issn.1006-2157.2014.03.010

• 针灸 • 上一篇    下一篇

艾灸对类风湿性关节炎大鼠炎症因子和滑膜细胞凋亡的影响

张传英, 蔡荣林, 唐照亮   

  1. 安徽中医药大学 安徽 230038
  • 收稿日期:2013-10-22 出版日期:2014-03-30 发布日期:2014-03-30
  • 作者简介:张传英,女,硕士,讲师
  • 基金资助:
    国家重点基础研究发展计划(973计划)项目(No.2009CB522905), 安徽省自然科学基金项目(No.1408085QH164)

Influences of moxibustion on inflammatory factors and synoviocytes in rats with rheumatoid arthritis

ZHANG Chuan-ying, CAI Rong-lin, TANG Zhao-liang   

  1. Anhui University of Traditional Chinese Medicine, Anhui 230038
  • Received:2013-10-22 Online:2014-03-30 Published:2014-03-30

摘要: 目的 探讨艾灸治疗类风湿性关节炎(RA)的作用机理。方法 40只Wistar大鼠随机分为正常组、模型组、艾灸组、针刺组、红外组,每组8只。观察并检测各组大鼠足跖肿胀度,血清白细胞介素1、2(IL-1、IL-2)的含量和关节滑膜组织细胞凋亡抑制基因(Bcl-2)、半胱氨酸蛋白酶(Caspase-3)蛋白表达情况。结果 模型大鼠足跖与踝部呈急性炎症性红肿,跖围较正常组明显增粗,血清中IL-1含量升高,IL-2水平降低,Bcl-2、Caspase-3蛋白表达均升高(P<0.05)。经治疗后,艾灸组、针刺组和红外组跖围减小,血清中IL-1含量降低,IL-2水平升高,Bcl-2蛋白表达明显下降(P<0.05),Caspase-3蛋白表达升高(P<0.05)。结论 艾灸肾俞穴能抑制RA大鼠继发性足肿胀,抑制血清中致炎因子IL-1的释放,提高免疫调节因子IL-2含量,增强RA大鼠滑膜组织的Caspase-3蛋白表达,降低Bcl-2蛋白表达。

关键词: 艾灸, 类风湿关节炎, 细胞凋亡抑制基因, 半胱氨酸蛋白酶-3, 大鼠

Abstract: Objective To discuss the mechanism of moxibustion in treating rheumatoid arthritis (RA). Methods Wistar rats (n=40) were randomly divided into normal group, model group, moxibustion group, acupuncture group and infrared group (each n=8). The degree of plantar swelling was observed and content of serum IL-1 and IL-2, and protein expressions of Bcl-2 and Caspase-3 in knee synomium were detected in rats of all groups. Results In model group, plantar and ankle inflammatory swelling was observed, plantar circumference was significantly longer compared with normal group, IL-1 content increased, IL-2 decreased, and expressions of Bcl-2 and Caspase-3 increased (P<0.05). After treatment, plantar circumference was shorter, IL-1 content decreased, IL-2 increased, expression of Bcl-2 decreased (P<0.05), and expression of Caspase-3 increased (P<0.05) in moxibustion group, acupuncture group and infrared group. Conclusion Moxibustion on Shenshu (BL23) can inhibit secondary plantar swelling and release of IL-1, increase IL-2 content, improve the expression of Caspase-3 and decrease expression of Bcl-2 in knee synomium in RA rats.

Key words: moxibustion, rheumatoid arthritis, Bcl-2, Caspase-3, rats

中图分类号: 

  • R245.81