主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

北京中医药大学学报 ›› 2014, Vol. 37 ›› Issue (4): 247-251.doi: 10.3969/j.issn.1006-2157.2014.04.008

• 科技之窗 • 上一篇    下一篇

益气化痰活血方对NOD.H-2小鼠甲状腺Th17细胞分化影响的研究

杨潇1, 高天舒2, 周喜玉2, 徐佳2, 曲金桥2   

  1. 1 辽宁中医药大学附属第二医院 辽宁 110034;
    2 辽宁中医药大学附属医院
  • 收稿日期:2013-10-12 出版日期:2014-04-30 发布日期:2014-04-30
  • 通讯作者: 高天舒,男,教授,博士生导师,主要研究方向:中西医结合防治甲状腺疾病的研究,E-mail:gaotianshu67@163.com
  • 作者简介:杨潇,女,在读博士生,主治医师
  • 基金资助:
    教育部博士点基金资助项目(No.20092133110004);辽宁省教育厅优秀人才计划资助项目(No.LR201026);沈阳市科技局计划资助项目(No.F10-205-1-31)

Influence of Yiqi Huatan Huoxeu Fang on differatiation of Th17 cells in NOD.H-2 mice

YANG Xiao1, GAO Tian-shu2, ZHOU Xi-yu2, XU Jia2, QU Jin-qiao2   

  1. 1 Second Affiliated Hospital of Liaoning University of Traditional Chinese Medicine, Liaoning 110034;
    2 Affiliated Hospital of Liaoning University of Traditional Chinese Medicine
  • Received:2013-10-12 Online:2014-04-30 Published:2014-04-30

摘要: 目的 探讨益气化痰活血方对NOD.H-2h4小鼠甲状腺白细胞介素-23(IL-23)/白细胞介素-17(IL-17)炎症轴的影响。方法 选雌性8周龄NOD.H-2h4小鼠,随机分成正常组、模型组、益气化痰活血方低剂量组(20.8 g/kg)和高剂量组(62.4 g/kg)。正常组饲以双蒸水,其余各组饲以含0.05%碘化钠水,8周后制成自身免疫性甲状腺炎 (AIT)动物模型,再处理因素8周后麻醉处死小鼠。放射免疫分析法(RIA)检测血清总T3(TT3)、总T4(TT4),固相化学发光酶免疫法(IRMA法)检测血清促甲状腺激素(TSH);光镜下观察甲状腺细胞形态;免疫组化方法测定甲状腺间质细胞CD68及小鼠甲状腺IL-17、IL-23蛋白表达;RT-PCR法检测小鼠甲状腺IL-17、IL-23 mRNA表达;流式细胞仪分析小鼠脾细胞Th17细胞比例。结果 各组间血清TT3﹑TT4及TSH值比较无明显差异(P>0.05);与正常组比较,模型组AIT的发生率、CD68、IL-17、IL-23表达及Th17细胞比例显著升高(P<0.01);与模型组比较,益气化痰活血方不同剂量组CD68、IL-17、IL-23表达显著下降(P<0.01),Th17细胞比例降低(P<0.01或P<0.05)。结论 益气化痰活血方治疗AIT的机制可能与其抑制IL-23/IL-17炎症轴,下调Th17细胞比例有关。

关键词: 益气化痰活血方, 自身免疫甲状腺炎, Th17细胞, 小鼠

Abstract: Objective To investigate the influence of Yiqi Huatan Huoxeu Fang on inflammatory axis of thyroid interlukin-23 (IL-23)/interlukin-17 (IL-17) in NOD.H-2h4 mice. Methods Female NOD.H-2h4 mice (8 weeks old) were chosen and divided into normal group, model group, low-dose Yiqi Huatan Huoxeu Fang group (low-dose group, 20.8 g/kg) and high-dose Yiqi Huatan Huoxeu Fang group (high-dose group, 62.4 g/kg). Normal group was given double distilled water and other groups, Nal water (0.05%). After 8 weeks, the model of autoimmune thyroiditis (AIT) was established and intervened for 8 weeks. The levels of serum total T3 (TT3) and total T4 (TT4) were detected by using radioimmunoassay (RIA), and level of serum thyroid stimulating hormone (TSH) was detected by using solid-phase chemi-luminescent enzyme immunoassay. The morphological changes of thyroid cells were observed with light microscope. The protein expressions of thyroid mesenchymal CD68, and thyroid IL-17 and IL-23 were detected by using immunohistochemistry technique, mRNA expressions of IL-17 and IL-23 were detected by using RT-PCR, and percentage of splenocyte Th17 was analyzed by using flow cytometry. Results The levels of TT3, TT4 and TSH had no significant difference compared among all groups (P>0.05). The incidence of AIT, expressions of CD68, IL-17 and IL-23 and percentage of Th17 all increased significantly in model group compared with normal group (P<0.01). The expressions of CD68, IL-17 and IL-23 decreased significantly (P<0.01) and percentage of Th17 decreased (P<0.01 or P<0.05) in high-dose group and low-dose group compared with model group. Conclusion The mechanism of Yiqi Huatan Huoxeu Fang for treating AIT may be related to that it can inhibit the inflammatory axis of IL-23/IL-17 and down-regulate percentage of Th17.

Key words: Yiqi Huatan Huoxeu Fang, autoimmune thyroiditis, Th17 cells, mice

中图分类号: 

  • R285.5