主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

北京中医药大学学报 ›› 2014, Vol. 37 ›› Issue (5): 337-343.doi: 10.3969/j.issn.1006-2157.2014.05.012

• 中药化学 • 上一篇    下一篇

基于药物体系的连翘饮药物制备物质量表征分析研究

梅莹莹1, 许舒娅1, 左胜1, 彭平1, 权建野1, 安琪2, 姜艳艳1, 王春梅1, 王永炎3, 石任兵1,4   

  1. 1 北京中医药大学中药学院 国家中医药管理局中药经典名方有效物质发现重点实验室 北京 100102;
    2 首都医科大学中医药学院;
    3 中国中医科学院;
    4 北京市教委中药质量控制技术工程中心
  • 收稿日期:2014-01-04 出版日期:2014-05-30 发布日期:2014-05-30
  • 通讯作者: 石任兵,男,博士,教授,博士生导师,研究方向:中药(复方)药效物质基础及药物创新研究,E-mail:shirb@126.com
  • 作者简介:梅莹莹,女,在读硕士生
  • 基金资助:
    十二五国家科技支撑计划项目(No.2012BA129B06);北京中医药大学创新团队项目(No.2011-CXTD-12);北京中医药大学重点学科开放课题(No.2013-ZDXKKF-23)

Quality-representation of medicinal-preparation of Lianqiao Yin based on medicinal system

MEI Ying-ying1, XU Shu-ya1, ZUO Sheng1, PENG Ping1, QUAN Jian-ye1, AN Qi2, JIANG Yan-yan1, WANG Chun-mei1, WANG Yong-yan3, SHI Ren-bing1,4   

  1. 1 School of Chinese Pharmacy, Key Unit of Exploring Effective Substances of Classical and Famous Formulas of State Administration of Traditional Chinese Medicine, Beijing University of Chinese Medicine,Beijing 100102;
    2 School of Traditional Chinese Medicine, Capital University of Medical Sciences;
    3 China Academy of Chinese Medical Sciences;
    4 Quality Control Technology and Engineering Center of Chinese Medicine, Beijing Municipal Commission of Education
  • Received:2014-01-04 Online:2014-05-30 Published:2014-05-30

摘要: 目的 基于药物体系建立连翘饮制备物有效指标性成分的含量测定方法,进行药物制备物质量表征关联分析研究,并确定影响质量的关键因素,为创制连翘饮优质抗菌药物奠定基础。方法 采用所建立的HPLC-DAD分析法测定连翘饮制备物有效指标性成分的含量。体外抗菌作用强度考察采用纸片扩散法,观察抑菌圈直径大小的变化;体内抗菌作用考察,采用金黄色葡萄球菌的5%干酵母悬液,腹腔感染给药小鼠,观察小鼠在染菌后7 d内的死亡率和平均存活时间。结果 连翘饮药物制备物有效指标性成分即栀子苷、升麻素苷、升麻素、甘草苷、连翘酯苷A 、5-O-甲基维斯阿米醇苷、连翘苷、甘草酸的线性关系良好,加样回收率合格。3种药物制备物体内、外均表现出抗菌作用,抗菌作用大小为:富集物>稀醇提取物>水提取物。连翘饮药物制备物药学-药效质量表征关联分析可知,在3种制备物中,尤以富集物中有效指标性成分的含量较高、比例独特、出膏率较低,药物用量较小,抗菌作用较强。结论 基于药物体系建立的连翘饮有效指标性成分的含量测定方法操作简便、准确灵敏、重复性好,可用于连翘饮药物制备物质量表征研究。基于药物体系的基本组成及其制备得率,提高关键有效成分的含量,优化组成比例,是提升药物药效的关键要素。

关键词: 药物体系, 连翘饮药物制备物, 质量表征, 药学-药效关联性分析

Abstract: Objective To establish the method of quantitative determination for effective index components of medicinal-preparation of Lianqiao Yin based on medicinal system, analyze quality-representation correlation, insure key factors influencing quality and prepare antimicrobial agents with high quality from Lianqiao Yin. Methods The content of effective index components of medicinal-preparation of Lianqiao Yin was detected by using HPLC-DAD. According to the size of the antibacterial annulus, use paper disk method to determine the antibacterial effect of drug-preparation in vitro. In vivo, Staphylococcus aureus was cultured for 18 hours and 5% yeast liquor was inoculated into the abdominal cavity of mice who had given medicine. Death rate and average survival time were observed and analyzed within 7days after being infected. Results The effective index components gardenosid(GD); prim-O-glucosylcimifugin(PGCN); cimifugin(CN); liquiritin(LQR); forsythoside A(FTA);4’-O-D-glucosyl-5-O-methylvisamminol(GMV);phillyrin(Phil); glycyrrhizic acid(GZA), of medicinal-preparation of Lianqiao Yin showed a good linear relationship, and qualified average recovery. All 3 kinds of medicinal-preparation had antibacterial effects in vivo and in vitro, and their descending order was as follows: enrichment>alcohol extract>water extract. According to the medicine-efficacy correlation analysis, among 3 medicinal-preparation, the content of effective index components is higher in enrichment with unique proportion, lower extraction rate, lower dosage and higher antibacterial effect, which is the best medicinal-preparation application form of Lianqiao Yin. Conclusion The method is simple, accurate and sensitive with good repetition, which can be used in the study on quality-representation of medicinal-preparation of Lianqiao Yin. Based on the basic composition and extraction rate of medicinal system, the key factor for increasing medicinal efficacy is to promoting relative effective components and optimizing their proportion.

Key words: medicinal system, medicinal-preparation of Lianqiao Yin, quality-representation, medicine-efficacy correlation analysis

中图分类号: 

  • R284.2