主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

北京中医药大学学报 ›› 2014, Vol. 37 ›› Issue (7): 481-485.doi: 10.3969/j.issn.1006-2157.2014.07.010

• 中药化学 • 上一篇    下一篇

基于药物体系的藁本质量评价研究

左胜1, 孙雅姝1, 迟蕾1, 梅莹莹1, 安琪3, 姜艳艳1,2, 张硕峰1, 周永强5, 张晓雷5, 王永炎4, 石任兵1,2   

  1. 1 北京中医药大学中药学院 国家中医药管理局中药经典名方有效物质发现重点实验室 北京 100102;
    2 北京市教委中药质量控制技术工程中心;
    3 首都医科大学中医药学院;
    4 中国中医科学院;
    5 上海绿谷制药有限公司
  • 收稿日期:2014-03-26 出版日期:2014-07-30 发布日期:2014-07-30
  • 通讯作者: 石任兵,男,博士,教授,博士生导师,主要研究方向:中药(复方)有效物质基础研究与药物创新,E-mail:shirb@126.com
  • 作者简介:左胜,男,在读硕士生
  • 基金资助:
    国家十二五科技支撑计划项目(No.2012BAI29B06);北京中医药大学创新团队资助项目(No.2011-CXTD-12);北京中医药大学重点学科开放课题(2013-2DXKKF-23)

Quality reviewing of Gaoben (Rhizoma Ligustici) based on drug system

ZUO Sheng1, SUN Ya-shu1, CHI Lei1, MEI Ying-ying1, AN Qi3, JIANG Yan-yan1,2, ZHANG Shuo-feng1, ZHOU Yong-qiang5, ZHANG Xiao-lei5, WANG Yong-yan4, SHI Ren-bing1,2   

  1. 1 Key Unit of Exploring Effective Substances of Classical and Famous Formulas of State Administration of Traditional Chinese Medicine, School of Chinese Pharmacy, Beijing University of Chinese Medicine; Beijing 100029;
    2 Quality Control Technology and Engineering Center of Chinese Medicine, Beijing Municipal Commission of Education;
    3 School of Traditional Chinese Medicine, Capital University of Medical Sciences;
    4 China Academy of Chinese Medical Sciences;
    5 Shanghai Green Valley Pharmaceutical Co., Ltd.
  • Received:2014-03-26 Online:2014-07-30 Published:2014-07-30

摘要: 目的 建立基于药物体系的藁本质量表征及其关联分析方法,为中药质量有效评价提供新模式。方法 采用HPLC同时测定7批藁本饮片中阿魏酸、洋川芎内酯A、洋川芎内酯H、洋川芎内酯I、藁本内酯等5种有效指标性成分的含量,分析基于有效指标性成分含量的质量表征;以洋川芎内酯I含量为参比,对饮片进行有效指标性成分含量相对比值质量表征,并以批次4(已确定药效的饮片)为基准,作有效指标性成分含量相对比值的比值差,其绝对值之和表示为非关联系数δ,分析得关联度排序(δ/关联度)。结果 基于有效指标性成分含量的质量表征:以阿魏酸含量排序为批次7>批次6>批次4>批次3>批次1>批次5>批次2,含量相差最大约2.2倍;以洋川芎内酯I含量排序为批次7>批次4>批次3>批次6>批次1>批次5>批次2,含量相差最大约4.9倍;以苯酞类含量和排序为批次7>批次3>批次6>批次4>批次1>批次2>批次5,含量相差最大约3.6倍;批次7、3、4、6有效指标性成分总体含量较高。基于关键有效指标性成分含量相对比值的质量表征:批次4(0/100%)>批次7(1.21/79.8%)>批次3(1.36/77.3%)>批次6(1.83/69.5%)>批次5(2.18/63.7%)>批次1(2.28/62.0%)>批次2(9.14),即批次7、3的质量表征与批次4关联度较高。综合基于有效指标性成分含量及其相对比值的质量表征,以批次4为基准,可得批次7、3质量较优。结论 基于有效指标性成分含量及其相对比值进行质量表征,并以具有确切药效的药材饮片为基准,进行质量表征关联度分析,以评价其质量整体关联性与应用有效性,这是中药质量评价提供一种新模式。

关键词: 藁本, 质量评价, 关联分析, 阿魏酸, 洋川芎内酯A, 洋川芎内酯H, 洋川芎内酯I, 藁本内酯

Abstract: Objective To establish the quality representation of Gaoben (Rhizoma Ligustici) and correlation analysis method based on drug system, and provide a new mode for quality evaluation of Chinese medicinal. Methods HPLC method was used for simultaneous determination of 5 effective index components in 7 batches of Gaoben decocting pieces, including ferulic acid, senkyunolide A, senkyunolide H, senkyunolide I, and ligustilide, and then a correlation analysis was carried out;The quality representation based on the content relative ratio of key effective index components showed as follows: taking the content of senkyunolide I as reference, the quality representations of decocting pieces were determined based on the content relative ratio of effective index components, and using batch 4 (decocting pieces with identified therapeutic effect) as baseline for getting the difference between these relative ratio of effective index components, and its sum of absolute value represented as non-correlation coefficient. Results The quality representation based on the content of effective index components showed as follows: by the content of ferulic acid, the sorting was batch 7>batch 6>batch 4>batch 3>batch 1>batch 5>batch 2 and the maximum content difference was about 2.2 times; by the content of senkyunolide I, the sorting was batch 7>batch 4>batch 3>batch 6>batch 1>batch 5>batch 2 and the maximum content difference was about 4.9 times; by the total content of phthalides, the sorting was batch 7>batch 3>batch 6>batch 4>batch 1>batch 2>batch 5 and the maximum content difference was about 3.6 times. The total content of effective index components was higher in batch 7, batch 3, batch 4 and batch 6. The sorting of the correlation degree ( δ/correlation degree) was analyzed as batch 4 (0/100%)>batch 7 (1.21/79.8%)>batch 3 (1.36/77.3%)>batch 6 (1.83/69.5%)>batch 5 (2.18/63.7%)>batch 1 (2.28/62.0%)>batch 2 (9.14). The results indicated that the correlation degree was higher between the quality representations of batch 7 or batch 3 and that of batch 4. Conclusion The analysis on the correlation degree of quality representation is conducted based on the content of effective index components and content relative ratio and taking decocting pieces with identified therapeutic effects as baseline for evaluating the overall correlation of quality and the effectiveness of application.

Key words: Gaoben (Rhizoma Ligustici), quality evaluation, correlation analysis, ferulic acid, senkyunolide A, senkyunolide H, senkyunolide I, ligustilide

中图分类号: 

  • R284.1