主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

北京中医药大学学报 ›› 2014, Vol. 37 ›› Issue (7): 486-489.doi: 10.3969/j.issn.1006-2157.2014.07.011

• 科技之窗 • 上一篇    下一篇

丹栀逍遥散对饮水冲突天敌暴露模型大鼠行为学影响

王雯龙, 唐启盛, 赵瑞珍, 徐硕, 杨歆科, 黄翔   

  1. 北京中医药大学第三附属医院 北京 100029
  • 收稿日期:2014-03-18 出版日期:2014-07-30 发布日期:2014-07-30
  • 通讯作者: 唐启盛,男,博士, 教授,主任医师,博士生导师,主要研究方向:中医药防治脑病,E-mail:tangqisheng@ 263. net
  • 作者简介:王雯龙,男,在读硕士生
  • 基金资助:
    国家自然科学基金项目(No. 81173231) ;北京中医药大学创新团队项目(No. 2011-CXTD-26)

Effect of Danzhi Xiaoyao San on ethology of rat model in Vogel's drinking conflict test and predator exposing test

WANG Wen-long, TANG Qi-sheng, ZHAO Rui-zhen, XU Shuo, YANG Xin-ke, HUANG Xiang   

  1. Third Affiliated Hospital of Beijing University of Chinese Medicine, Beijing 100029
  • Received:2014-03-18 Online:2014-07-30 Published:2014-07-30

摘要: 目的 探讨丹栀逍遥散对广泛性焦虑障碍的作用机制。方法 观察丹栀逍遥散对饮水冲突联合天敌暴露应激焦虑模型大鼠行为学的影响。30只大鼠随机分为正常组、模型组、中药组,于造模结束前7 d开始中药干预。除正常组外,其余2组采用饮水冲突法联合天敌暴露法建立应激焦虑大鼠模型,期间2种造模方法交替进行20 d。最后1次应激结束之后,于24 h后记录观察药物干预后,各组大鼠在旷场实验和高架十字迷宫测试结果的异同。 结果 最后1次复合应激结束24 h 之后,相对于正常组,模型组大鼠,水平、垂直运动次数减少,OE%和OT%比例下降;相对于模型组,中药组大鼠,水平、垂直运动次数增加,OE%和OT%比例上升,除OT%外以上结果均有显著差异。结论 丹栀逍遥散对于饮水冲突天敌暴露应激焦虑模型大鼠表现有明显抗焦虑作用。

关键词: 天敌暴露, 饮水冲突, 广泛性焦虑障碍, 丹栀逍遥散, 行为学, 大鼠

Abstract: Objective To investigate the effective mechanism of Danzhi Xiaoyao San to generalized anxiety disorder. Methods The influence of Danzhi Xiaoyao San on the ethology in rat model of stress anxiety in Vogel's drinking conflict test combining predator exposing test. All 30 rats were randomly divided into normal group, model group and Chinese medicinal group. The rat model of stress anxiety was established by using Vogel's drinking conflict test combining predator exposing test in model group and Chinese medicinal group for 20 d. After the last time of stress finished and 24-h medicinal intervention, the test results of open field test (OFT) and elevated plus maze (EPM) were compared among all groups. Results After the last time of combined stress for 24 h, the times of horizontal movement and vertical movement, and percentage of open arm entry (OE%) and percentage of open arm time (OT%) decreased in model group compared with normal group. The times of horizontal movement and vertical movement and OE% and OT% increased in Chinese medicinal group compared with model group. All the above results had significant difference except of OT%. Conclusion Danzhi Xiaoyao San has a significant anti-anxiety effect in rat model of stress anxiety in Vogel's drinking conflict test combining predator exposing test.

Key words: predator esposing, drinking conflict, generalized anexiety disorder, Danzhi Xiaoyao San, ethology, rats

中图分类号: 

  • R285.5