主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

北京中医药大学学报 ›› 2014, Vol. 37 ›› Issue (10): 691-695.doi: 10.3969/j.issn.1006-2157.2014.10.010

• 中医药实验研究 • 上一篇    下一篇

青黛对实验性结肠炎抗炎作用的体内外研究

刘丽娟1, 王志斌1, 王允亮1, , 许树青2, 魏仕兵1, 李军祥1   

  1. 1 北京中医药大学东方医院 北京 100078;
    2 解放军61785部队门诊部
  • 收稿日期:2014-04-03 出版日期:2014-10-30 发布日期:2014-10-30
  • 通讯作者: 李军祥,男,博士,主任医师,博士生导师,E-mail: lijunxiang1226@163.com
  • 作者简介:刘丽娟,女,在读博士生
  • 基金资助:
    北京中医药大学创新团队资助项目(No.2011-CXTD-24)

Anti-inflammatory effects of Qingdai powder on experimental ulcerative colitis in vivo and in vitro

LIU Li-juan1, WANG Zhi-bin1, WANG Yun-liang1 ,XU Shu-qing2, WEI Shi-bing1, LI Jun-xiang1   

  1. 1 Dongfang Hospital, Beijing University of Chinese Medicine, Beijing 100078;
    2 Department of Out-patient, PLA No.61785 Unit
  • Received:2014-04-03 Online:2014-10-30 Published:2014-10-30

摘要: 目的 研究青黛对溃疡性结肠炎(UC)体内、体外炎症模型的抗炎作用。方法 大鼠随机分为正常组、模型组、青黛高剂量组、青黛低剂量组,以低浓度TNBS/乙醇溶液灌肠诱导大鼠实验性结肠炎体内炎症模型,连续以青黛高、低剂量混悬液灌胃治疗10 d,酶联免疫吸附法(ELISA)检测血清白细胞介素-1(IL-1)、IL-6、IL-8浓度。以脂多糖(LPS)刺激小鼠巨噬细胞株RAW264.7细胞24 h诱导体外炎症模型,以MTT法检测青黛对细胞生存率的影响,以ELISA法研究青黛在直接干预、预防干预2种模式下对细胞培养液IL-6、肿瘤坏死因子-α(TNF-α)表达的影响。结果 动物实验:模型组大鼠血清中IL-1、IL-6、IL-8浓度均明显高于空白组(P<0.05),青黛高剂量组血清中IL-1、IL-6、IL-8含量均明显低于模型组(P<0.05),青黛低剂量组血清IL-6、IL-8浓度明显低于模型组(P<0.05)。细胞实验:模型组中IL-6、TNF-α水平较正常组均有明显升高(P<0.05);青黛直接干预时,10 μmol/L青黛组细胞培养液IL-6、TNF-α均较模型组有明显下降(P<0.05);青黛预防性干预时,10 μmol/L青黛组IL-6明显下降(P<0.05)。结论 青黛对溃疡性结肠炎有一定的体内、体外抗炎作用,抗炎机制可能为下调炎症因子的表达。

关键词: 青黛, 溃疡性结肠炎, 小鼠巨噬细胞株, 抗炎, 大鼠

Abstract: Objective To study anti-inflammatory effect of Qingdai powder on rat model of ulcerative colitis(UC) and on murine macrophage RAW264.7 cells. Methods Overall SD rats were randomly divided into 4 groups: normal group, model group, Qingdai high-dose group (high-dose group) and Qingdai low-dose group(low-dose group). Rat model of UC was induced by intracolonical administration of 2,4,6-trinitrobenzene sulfonic acid (TNBS)-ethanol solution. Qingdai suspension liquid was administered intragastrically once a day for 10 d. Serum concentration of IL-1,IL-6 and IL-8 was tested by ELISA assay. On the other hand, murine macrophage RAW264.7 cells were induced by 10μg/mL LPS for 24 h to establish an UC inflammatory cell model. Cell viability was detected by MTT assay to determine the safe range of concentrations. After direct and prophylactic intervention of high- , mid- and low-dose Qingdai suspension on RAW264.7 cell respectively, concentration of IL-6 and TNF-α in supernatant were tested by ELISA assay. Results Compared with normal group, serum concentration of IL-1,IL-6 and IL-8 in model group increased significantly (P<0.05); compared with model group, those of IL-1,IL-6 and IL-8 in high-dose group, and IL-6 and IL-8 in low-dose group decreased significantly(P<0.05). Compared with normal group, concentration of IL-6 and TNF-α in supernatant increased significantly in model group (P<0.05). After direct intervention, those of IL-6 and TNF-α in supernatant in 10 μmol/L Qingdai group reduced significantly(P<0.05) compared with model group; while after prophylactic intervention, only IL-6 in 10 μmol/L Qindai group reduced (P<0.05). Conclusion Effects of anti-inflammation of Qingdai showed both in vivo and in vitro, and the potential mechanism probably was through down-regulating the expression of inflammatory factors.

Key words: Qingdai, ulcerative colitis, rats, RAW264.7 cells, anti-inflammation

中图分类号: 

  • R285.5