主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

北京中医药大学学报 ›› 2014, Vol. 37 ›› Issue (11): 773-776.doi: 10.3969/j.issn.1006-2157.2014.11.012

• 科技之窗 • 上一篇    下一篇

益气化瘀解毒法对慢性萎缩性胃炎伴异型增生大鼠P21及CyclinD1的影响

贺梅娟1, 杨晋翔2, 安静1, 黄大未1, 贾云飞1   

  1. 1 北京中医药大学 北京 100029;
    2 北京中医药大学第三临床医学院
  • 收稿日期:2014-05-07 出版日期:2014-11-30 发布日期:2014-11-30
  • 通讯作者: 杨晋翔,男,主任医师,教授,博士生导师,研究方向:脾胃病中医药防治研究,E-mail:yjx571102@163.com
  • 作者简介:贺梅娟,女,在读博士生
  • 基金资助:
    国家自然科学基金面上项目(No.81173232);北京中医药大学在读研究生资助项目

Effects on P21WAF1 and CyclinD1 in chronic atrophic gastritis with dysplasia in rats: TCM therapy of replenishing qi, resolving stasis and detoxification

HE Mei-juan1 , YANG Jin-xiang2, AN Jing1, HUANG Da-wei1, JIA Yun-fei1   

  1. 1 Beijing University of Chinese Medicine, Beijing 100029;
    2 Third Affiliated Hospital, Beijing University of Chinese Medicine
  • Received:2014-05-07 Online:2014-11-30 Published:2014-11-30

摘要: 目的 观察益气化瘀解毒法对慢性萎缩性胃炎伴异型增生模型大鼠P21WAF1和CylinD1基因的影响。方法 将60只Wistar大鼠随机选取50只造模,其余10只为空白组。造模成功后将大鼠随机分为模型组、维酶素组、消痞颗粒组,每组各11只。模型组予生理盐水,维酶素组予维酶素悬浊液,消痞颗粒组予消痞颗粒中药制备药液,1次/d。连续治疗12周后用实时荧光定量PCR法(RT-PCR)及Western blot法对各组大鼠P21WAF1和细胞周期素D1(CyclinD1)基因进行检测。结果 P21WAF1mRNA及蛋白表达量消痞颗粒组较模型组及维酶素组显著增高(P<0.05)。CyclinD1 mRNA及蛋白表达量消痞颗粒组较模型组、维酶素组显著降低(P<0.05)。结论 中医益气化瘀解毒法可以逆转胃黏膜异型增生,这种作用可能是通过促进P21WAF1的表达、抑制CyclinD1的表达,从而恢复P21-CDK-CyclinD1之间的平衡,进而使细胞周期调控恢复正常而实现的。

关键词: 慢性萎缩性胃炎伴异型增生, 益气化瘀解毒法, P21WAF1, CyclinD1, 大鼠

Abstract: Objective To observe the effects of Xiaopi granule on gene P21WAF1 and gene CyclinD1, which was formulated by replenishing qi, resolving stasis and detoxification, in chronic atrophic gastritis with dysplasia in rats. Methods 50 of 60 Wistar rats were randomly selected to be modeled, and the rest of rats in blank group (n=10). After establishment of model, the alive rats (n=33) were randomly divided into model group, vitacoenzyme tablet group (western medicinal group) and Xiaopi granule group(Chinese medicinal group) (each n=11). Model group, western medicinal group and Chinese medicinal group were intragastrically administered once a day saline water, vitacoenzyme and Xiaopi granule respectively for 12 consecutive weeks. Real-time fluorescence quantitative polymerase chain reaction (RT-PCR) and Western blot were used to determine the expressions of P21WAF1 and CyclinD1 genes in rats of all groups. Results The expression of P21WAF1mRNA and protein significantly increased in Chinese medicinal group compared with model group and western medicinal group (P<0.05). The expression of CyclinD1 mRNA and protein dramatically decreased in Chinese medicinal group compared with model group and western medicinal group (P<0.05). Conclusion Chinese medicine Xiaopi granule can reverse the gastric mucosal dysplasia, due to increasing the expression of P21WAF1 and inhibition the expression of CyclinD1, leading to redressing the balance of P21-CDK-CyclinD1 pathway.

Key words: chronic atrophic gastritis with gastric dysplasia, replenishing qi, resolving stasis and detoxification, P21WAF1, CyclinD1, rats

中图分类号: 

  • R285.5