主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

北京中医药大学学报 ›› 2015, Vol. 38 ›› Issue (3): 190-194.doi: 10.3969/j.issn.1006-2157.2015.03.010

• 科技之窗 • 上一篇    下一篇

益气活血方和补肾生髓方对局灶性脑缺血再灌注大鼠海马Wnt3a、Frizzled9和TCF3表达的影响*

宋祖荣1,王键2,胡建鹏2#,徐伟2   

  1. 1 安徽新华学院药学院 安徽 230088;
    2 安徽中医药大学省部共建新安医学教育部重点实验室
  • 收稿日期:2014-09-23 出版日期:2015-03-28 发布日期:2015-03-28
  • 通讯作者: 胡建鹏,男,博士,教授,博士生导师,E-mail:hujianpeng351@126.com
  • 作者简介:宋祖荣, 女, 硕士,讲师
  • 基金资助:
    *国家科技支撑计划项目(No.2012BAI26B01),安徽省科技专项资金项目(No.13Z04013),安徽省教育厅自然研究重点项目(No.KJ2014A098)

Effects of Yiqi Huoxue formula and Bushen Shengsui formula on expression of Wnt3a,Frizzled9 and TCF3 in hippocampus of rats with ischemia-reperfusion injury*

SONG Zu-rong1,WANG Jian2,HU Jian-peng2#,XU Wei2   

  1. 1 College of Pharmacy, Anhui Xinhua University,Anhui 230088;
    2 Key Laboratory of Xin’an Medicine Jointly Developed by Ministry of Education and Anhui Province, Anhui University of Traditional Chinese Medicine
  • Received:2014-09-23 Online:2015-03-28 Published:2015-03-28

摘要: 目的 探讨益气活血方和补肾生髓方对脑缺血大鼠海马Wnt/β-catenin信号通路Wnt3a、卷曲蛋白9(Frizzled9)和T细胞因子3(TCF3)蛋白表达的影响。方法 采用线栓法制作局灶性脑缺血大鼠模型,随机分为假手术组、模型组、益气活血方组和补肾生髓方组,脑缺血2 h后持续灌注7 d。采用Western-Blot法检测缺血侧海马Wnt3a、Frizzled9和TCF3蛋白表达,用免疫组化Envision两步法检测缺血侧海马CA1区Wnt3a、Frizzled9和TCF3蛋白表达。结果 在缺血侧海马区,与假手术组比较,模型组Wnt3a、Frizzled9和TCF3蛋白的相对表达量均显著增加(P<0.01);与模型组比较,益气活血方组Wnt3a、Frizzled9和TCF3蛋白的相对表达量均显著降低(P<0.01),补肾生髓方组Wnt3a、Frizzled9和TCF3蛋白的相对表达量均显著降低(P<0.01或P<0.05)。在缺血侧海马CA1区,与假手术组比较,模型组Wnt3a、Frizzled9和TCF3蛋白表达显著增加(P<0.01);与模型组比较,益气活血方组与补肾生髓方组能显著降低CA1区Wnt3a、Frizzled9和TCF3蛋白表达(P<0.01)。结论 2种中药复方能通过抑制缺血侧海马Wnt3a、Frizzled9和TCF3蛋白表达,调节Wnt/β-catenin信号通路,促进局灶性脑缺血再灌注损伤脑组织修复。

关键词: 脑缺血再灌注, 益气活血方, 补肾生髓方, 海马, Wnt信号通路, 卷曲蛋白9, T细胞因子3, 大鼠

Abstract: Objective To study the effects of Yiqi Huoxue formula (YQHX) and Bushen Shengsui formula (BSSS) on the protein expression of Wnt3a,Frizzled 9 and TCF3 on Wnt/β-catenin pathway in hippocampus of rat model induced by middle cerebral artery ischemia/reperfusion. Methods The focal cerebral ischemia/ reperfusion rat model was established by intraluminal thread occlusion of the middle cerebral artery (MCAO)for 2-h followed by 7-d reperfusion. The rats were randomly divided into sham group, model group, YQHX group and BSSS group(each n=10). The expression of Wnt3a, Frizzled9 and TCF3 protein in hippocampus of cerebral ischemia were detected by Western-Blot assay,and those in CA1 zone of cerebral ischemia were measured by using immunohistochemistry Envision two-step method. Results The relative expression of Wnt3a,Frizzled 9 and TCF3 protein in both hippocamous and CA1 zone increased significantly in model group compared with sham group (P<0.01). Compared with model group, the expression of those three proteins in both hippocamous and CA1 zone decreased significantly in YQHX group and BSSS group (P<0.05). Conclusion Yiqi Huoxue formula and Bushen Shengsui formula can promote the repairment of injured cerebral tissue by the way of reducing the protein expression of Wnt3a,Frizzled9 and TCF3 in hippocampus to regulate Wnt/β-catenin signal pathway.

Key words: cerebral ischemia-reperfusion injury, Yiqi Huoxue formula, Bushen Shengsui formula, hippocampus, Wnt3a, Frizzled9, TCF3, rats

中图分类号: 

  • R285.5