主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

北京中医药大学学报 ›› 2015, Vol. 38 ›› Issue (4): 236-240.doi: 10.3969/j.issn.1006-2157.2015.04.005

• 科技之窗 • 上一篇    下一篇

基于正交试验设计研究不同穴位及刺激量对肾阳虚大鼠下丘脑-垂体-甲状腺轴的影响*

闵友江1,姚海华1,张辉2,丁李立强2,闵志云2,裴佳2,程立红1#   

  1. 1 江西中医药大学附属医院 江西 330006;
    2 江西中医药大学
  • 收稿日期:2014-10-08 出版日期:2015-04-15 发布日期:2015-04-15
  • 通讯作者: 程立红,女,教授,主任中医师,研究方向:刺灸方法与临床研究
  • 作者简介:闵友江,男,医学博士,副教授,副主任中医师
  • 基金资助:
    *国家自然科学基金资助项目( No.81360562),江西省卫计委科技计划项目(No. 20155384),江西省教育厅科技项目(No.GJJ10559)

Influence of different acupoints and intensity of stimulus on HPT axis in rats with syndrome of kidney-yang deficiency: a study based on orthogonal experimental design*

MIN You-jiang1,YAO Hai-hua1,ZHANG Hui2,DINGLI Li-qiang2,MIN Zhi-yun2,PEI Jia2 ,CHENG Li-hong1#   

  1. 1 Affiliated Hospital of Jiangxi University of Traditional Chinese Medicine, Jiangxi 330006;
    2 Jiangxi University of Traditional Chinese Medicine
  • Received:2014-10-08 Online:2015-04-15 Published:2015-04-15

摘要: 目的 采用正交试验设计研究影响针刺效应的因素,探索影响针刺效应的主次因素和针刺参数的最佳组合,以期为提高针刺疗效、指导针灸临床提供实验依据。方法 氢化可的松肌肉注射造皮质酮肾阳虚大鼠模型。采用L9(34)正交表对影响针刺效应的4种因素(穴位、捻针角度、捻针频率、捻针时间)3水平安排试验,运用计算机控制的针刺手法仪治疗肾阳虚大鼠,通过ELISA检测血清中T3、促甲状腺激素(TSH)的水平和RCT-PCR检测下丘脑组织中促甲状腺激素释放激素(TRH) mRNA的表达作为考察指标。结果 对针刺效应的影响大小依次是捻针频率、捻针时间、腧穴选择、捻针角度。捻针频率是影响针刺效应的主要因素(P<0.01);捻针时间是影响针刺效应的主要因素或重要因素(P<0.01或P<0.05);穴位是影响针刺效应的重要因素(P<0.05);捻针角度是影响针刺效应的次要因素(P>0.05)。治疗肾阳虚大鼠的最佳针刺参数为刺肾俞穴,捻针角度为90°,频率为120次/min,捻针时间60 s或120 s。结论 捻针频率和捻针时间是影响针刺效应的主要因素或重要因素,是构成针刺刺激量的主要要素。

关键词: 正交试验设计, 肾阳虚, T3, 促甲状腺激素, 促甲状腺激素释放激素 mRNA, 大鼠

Abstract: Objective To study the operational parameters of acupuncture treatment for rats model of kidney-yang dificiency syndrome, with serum T3, TSH and hypothalamic TRH mRNA as indicators, and to find the main influencing factors and an optimal combination of factors so as to provide experimental evidence. Methods Rat model of kidney-yang deficiency syndrome was induced by intramuscular injection of hydrocortisone. L9 (34 ) orthogonal experiment was designed with four factors viz. acupoint, angle of twisting-rotation(angle), frequency of twisting-rotation (frequency), duration of twisting-rotation (duration) at three levels respectively. The rats were treated by acupuncture manipulation device controlled by computer. Serum T3 and TSH were measured by using ELISA, and the expression of hypothalamic TRH mRNA was determined by using RT-PCR. Results Frequency was the primary factor (P<0.01), duration was the secondary factor(P< 0.01 or P<0.05) and acupoint was the tertiary factor(P< 0.05 ). The optimal acupuncture factors were acupoint of Shenshu (BL23) , angle of 90°, frequency of 120 times/min and duration of 60 s or 120 s. Conclusion Frequency and duration were the essential factors of intensity of acupuncture, which were also the important factors to influence of acupuncture.

Key words: orthogonal experimental design, kidney-yang deficiency, T3, TSH, TRH mRNA, rats

中图分类号: 

  • R285.5