主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

北京中医药大学学报 ›› 2015, Vol. 38 ›› Issue (4): 241-246.doi: 10.3969/j.issn.1006-2157.2015.04.006

• 中药化学 • 上一篇    下一篇

基于药物体系的紫苏子特征图谱质量表征关联分析研究*

唐雪阳1,安琪2,孙道涵1,温静2,卢广英1,许舒娅1,黄亚婷1,李钢1,姜艳艳1,3,石任兵1,3#   

  1. 1 北京中医药大学中药学院北京国家中医药管理局中药经典名方有效物质发现重点实验室 北京 100102;
    2 首都医科大学中医药学院;
    3 北京市教委中药质量控制技术工程中心
  • 收稿日期:2014-12-06 出版日期:2015-04-15 发布日期:2015-04-15
  • 通讯作者: 石任兵,男,博士,教授,博士生导师,研究方向:中药(复方)有效物质基础研究与药物创新,E-mail:shirb@126.com
  • 作者简介:唐雪阳,女,在读硕士生
  • 基金资助:
    *十二五国家科技支撑计划项目(No.2012BAI29B06),北京中医药大学创新团队资助项目(No.2011-CXTD-12)

Quality representation and correlation analysis of Fructus Perillae based on characteristic spectrum of medicinal system*

TANG Xue-yang1 , AN Qi2, SUN Dao-han1, WEN Jing2 , LU Guang-ying1, XU Shu-ya1, HUANG Ya-ting1, LI Gang1, JIANG Yan-yan1,3 , SHI Ren-bing1,3#   

  1. 1 Key Unit of Exploring Effective Substances of Classical and Famous Formulas of State Administration of Traditional Chinese Medicine, School of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing 100102;
    2 College of Traditional Chinese Medicine, Capital Medical University;
    3 Quality Control Technology and Engineering Center of Chinese Medicine,Beijing Municipal Commission of Education
  • Received:2014-12-06 Online:2015-04-15 Published:2015-04-15

摘要: 目的 建立基于药物体系的紫苏子特征图谱质量表征关联分析方法与评价模式,有效、精准地评价紫苏子饮片质量。方法 运用所建立的HPLC-PDA法表征紫苏子药物体系特征图谱质量,基于特征图谱中特征峰的数量及所属化学类型对15批紫苏子饮片进行质的表征,基于特征图谱中特征指标性成分木犀草素、芹菜素和迷迭香酸的含量及黄酮类(以木犀草素表征)、酚酸类(以迷迭香酸表征)的含量对15批紫苏子饮片进行量的表征,并基于基准饮片将15批紫苏子饮片质与量的表征结果分别进行关联性分析。结果 以批号15的样品为基准饮片,紫苏子饮片特征图谱中共含有特征峰12个,其中黄酮类成分特征峰5个,酚酸类成分特征峰7个,15批饮片色谱图中均含有此12个特征峰。批号15、6、9、10、2、5、7样品特征指标性成分含量总体较高,批号6、12、11、7、4、14、10样品与基准饮片关联性最高,综合评价得出批号15、6、7、10、2、5、9、12、11、14样品优良度居前。结论 对15批紫苏子饮片进行特征图谱质量表征,所建立的HPLC法简便、准确。所评价建立的基于药物体系的紫苏子特征图谱质量表征关联分析模式,关注了紫苏子饮片质量整体关联性与应用有效性,能有效精准地评价紫苏子饮片质量。

关键词: 紫苏子, 特征图谱, 质量评价, 关联分析, 木犀草素, 芹菜素, 迷迭香酸

Abstract: Objective To establish the quality representation and correlation analysis method based on the characteristic spectrum of medicinal system of Fructus Perillae (Zisuzi), so as to evaluate quality of Zisuzi pieces effectively and accurately. Methods Using established HPLC-PDA method to characterize the quality of Zisuzi characteristic spectrum of medicinal system. The quality of 15 batches of Zisuzi pieces was represented according to the number and chemical type of characteristic peaks on characteristic spectrum, and the quantity of 15 batches of Zisuzi pieces was represented based on the content of three characteristic index components of luteolin, apigenin,rosemary acid, flavonoids (represented by luteolin) and phenolic acids (represented by rosemary acid). Correlation analysis of quality and quantity representations was then performed referring to the benchmark pieces. Results As the reference substance, characteristic spectrum of Lot.15 contains 12 characteristic peaks, 5 peaks of flavonoids and 7 peaks of phenolic acids, which all appeared on the chromatograms of 15 batches Zisuzi pieces.Lot.15,6,9,10,2,5,7 Zisuzi pieces had more effective index components. After comprehensive evaluation of more total effective index components of Lot.6, 12, 11, 7, 4, 14 ,10 and high correlation degree of Lot. 6, 12, 11, 7, 4, 14, 10 correlated with Lot. 15, the quality of Lot. 15, 6, 7, 10, 2, 5, 9, 12, 11,14 were judged to be better. Conclusion The established HPLC method by quality and quantity representation of characteristic spectrum of 15 batches of Zisuzi pieces is simple and accurate. And the evaluation mode of quantity and quality and their correlation based on Zisuzi medicinal system, concerning on systemic correlation and application validity, can evaluate the quality of Zisuzi pieces effectively and accurately.

Key words: Fructus Perillae (Zisuzi), characteristic spectrum, quality evaluation, correlation analysis, luteolin, apigenin , rosemary acid

中图分类号: 

  • R284.1