主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

北京中医药大学学报 ›› 2015, Vol. 38 ›› Issue (5): 332-338.doi: 10.3969/j.issn.1006-2157.2015.05.010

• 科技之窗 • 上一篇    下一篇

益气解毒中药对急性辐射损伤的防护作用研究*

高明泽,徐文慧,王天琪,马捷,李峰,胡素敏#   

  1. 北京中医药大学基础医学院 北京 100029
  • 收稿日期:2014-12-18 出版日期:2015-05-15 发布日期:2015-05-15
  • 通讯作者: 胡素敏,女,博士,教授,主要研究方向:辐射损伤中医药防护措施研究,E-mail:husm@bucm.edu.cn
  • 作者简介:高明泽,女,在读硕士生
  • 基金资助:
    *国家自然科学基金项目(No.11475035),北京中医药大学青年教师资助课题(No.522/0100604150),北京中医药大学协同创新计划-“航海中医药”协同创新中心(No.522/0100604299)

Protective effects of qi-tonifying toxin-removing Chinese medicinals against acute radiation injury*

GAO Ming-ze, XU Wen-hui, WANG Tian-qi, MA Jie, LI Feng, HU Su-min#   

  1. School of Pre-clinical Medicine, Beijing University of Chinese Medicine, Beijing 100029
  • Received:2014-12-18 Online:2015-05-15 Published:2015-05-15

摘要: 目的 研究以益气解毒为主要功效的中药复方对急性辐射损伤小鼠的防护作用。方法 设空白组、模型组、氨磷汀组、安多霖组和益气解毒中药复方高、低剂量组,给药14 d。第14天给药后,除空白组外,对其他各组小鼠利用4 Gy 60Co γ射线进行全身一次性照射。分别于照射后第3、7、14 天检测小鼠外周血白细胞数,胸腺指数,脾脏指数,脾脏病理切片,外周血T淋巴细胞CD4+/CD8+比值,肝脏超氧化物歧化酶(SOD)活力和丙二醛(MDA)含量。结果 γ射线全身照射后,模型组小鼠外周血白细胞数显著降低,免疫脏器指数显著下降,脾脏有明显的病理改变,CD4+/CD8+比值显著升高,SOD活力显著下降,MDA含量显著升高,机体免疫力下降;于照后7 d,SOD活力和MDA含量自行恢复至正常水平;于照后14 d脾脏指数恢复至正常水平。与模型组比较,给药组脾脏病理改变较轻;照后3 d,低剂量组小鼠肝SOD活力显著增强(P<0.01),MDA含量显著降低(P<0.05);照后7 d,低剂量组和高剂量组小鼠脾脏指数显著提高(P<0.05),低剂量组外周血T淋巴细胞CD4+/CD8+比值显著降低(P<0.05);照后14 d,低剂量组小鼠胸腺指数显著提高(P<0.05)。结论 低剂量的益气解毒中药复方对γ射线所致的小鼠急性辐射损伤具有一定的保护作用。

关键词: 益气解毒, 氨磷汀, 安多霖, 辐射防护, 小鼠

Abstract: Objective Qi-tonifying toxin-removing Chinese medicinals can tonify qi and remove toxin. This research studied its protective effects against acute radiation injury in mice. Methods There were six groups, including blank group, model group, high dose group of qi-tonifying toxin-removing Chinese medicinals, low dose group of qi-tonifying toxin-removing Chinese medicinals, amifostine group, and anduolin group. All of the above groups were administered for 14 days. At day 14 after drug administration, all groups of mice, except the blank group, were given 4 Gy 60Co gamma ray for total body irradiation to induce acute radiation injury. At day 3,7, and 14 after irradiation, the mice’s white blood cell count, thymus index, spleen index, spleen pathological section, the ratio of CD4+/CD8+ T lymphocyte of peripheral blood, SOD activity and MDA content of liver were examined respectively. Results After gamma ray irradiation in the model group, a significant reduction in white blood cell count were detected. Immune organ index of model group mice decreased significantly. There were also significant pathological changes in the spleen, and marked increase of CD4+/CD8+ ratio, indicating impaired immunity. SOD activity decreased while MDA content increased significantly. At day 7 after irradiation, SOD activity and MDA content returned to normal level; At day 14 after irradiation, the spleen index returned to normal level. Compared with the model group, the spleen pathological changes of treatment groups were less severe. At day 3 after irradiation, the activity of SOD in liver of low dose group was significantly enhanced (p<0.01); hepatic MDA content significantly decreased (p<0.05). At day 7 after irradiation, the spleen index in low dose group and high dose group mice significantly increased (p<0.05), and the ratio of CD4+/CD8+ T lymphocyte in peripheral blood of low dose group significantly reduced (p<0.05); At day 14 after irradiation, thymus index of low dose group significantly increased (p<0.05). Conclusion Low dose of formula has protective effects against sublethal doses of gamma ray induced acute radiation injury in mice.

Key words: qi-Tonifying and toxin-removing method, amifostine, anduolin, radiation protection, mice

中图分类号: 

  • R285.5