主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

北京中医药大学学报 ›› 2015, Vol. 38 ›› Issue (6): 426-429.doi: 10.3969/j.issn.1006-2157.2015.06.013

• 针灸 • 上一篇    下一篇

不同针刺刺激量对寒凝证类痛经大鼠子宫收缩强度及内分泌相关分子受体基因表达的影响*

许咏思1,赵国桢2,嵇波1#,齐丹丹1,郭孟玮1,任晓暄1,赵雅芳1,张露芬1,
李晓泓1,朱江1,张鹤1,宋艳1,刘亚利1,张平1   

  1. 1 北京中医药大学针灸推拿学院 北京 100029;
    2 首都医科大学
  • 收稿日期:2014-11-20 出版日期:2015-06-15 发布日期:2015-06-15
  • 通讯作者: 嵇波,女,博士,教授,博士生导师,研究方向:针灸作用规律与机理研究,E-mail:jibo678@263.net
  • 作者简介:许咏思,女,在读博士生
  • 基金资助:
    *国家重点基础研究发展计划(973计划)资助项目(No.2012CB518506, No.2006CB504503),国家自然科学基金面上资助项目(No.30973793),教育部博士点基金资助项目(No.20090013110005)

Effects of acupuncture with different stimuli intensity on uterine contraction and expression of endocrine-related molecular receptor genes in rats with dysmenorrhea of cold coagulation pattern*

HSU Wing-sze1, ZHAO Guo-zhen2, JI Bo2#, QI Dan-dan1, GUO Meng-wei1, REN Xiao-xuan1, ZHAO Ya-fang1, ZHANG Lu-fen1, LI Xiao-hong1, ZHU Jiang1, ZHANG He1, SONG Yan1, LIU Ya-li1, ZHANG Ping1   

  1. 1 School of Acupuacture, Moxibustion and TuinaBeijing University of Chinese Medicine, Beijing 100029;
    2 Capital University of Medical Sciences
  • Received:2014-11-20 Online:2015-06-15 Published:2015-06-15

摘要: 目的 观察不同刺激量针刺三阴交对寒凝证类痛经大鼠子宫张力及内分泌相关分子受体基因表达的影响,探寻不同针刺刺激量与靶器官效应之间的关系。方法 将动情期间雌性大鼠随机分为盐水组、寒凝证类痛经大鼠模型(模型组)、期望“得气”组(粗针深刺行针的治疗方法)、期望“不得气”组(细针浅刺不行针的治疗方法);期望“得气”组或期望“不得气”组均在成功制备模型基础上给予治疗,并于针刺进行时观察20 min内在体大鼠子宫收缩强度及大鼠子宫组织前列腺素EP2受体mRNA(PGEP2mRNA)、前列腺素F受体mRNA(PGFmRNA)表达量。结果 子宫收缩强度检测方面,与模型组比较,期望“得气”组显著减少了子宫收缩波个数(P<0.01),显著降低子宫活动度(P<0.05)。子宫PGEP2mRNA及PGFmRNA检测方面,与模型组比较,期望“得气”组及期望“不得气”组 PGFmRNA 表达显著降低 (P<0.01)。结论 不同刺激量针刺三阴交穴均能缓解寒凝证类痛经大鼠子宫收缩强度及降低子宫内分泌相关分子受体基因表达,使用粗针深刺加行针的治疗效果优于细针浅刺不行针,认为粗针深刺加行针的刺激量更能达到针灸“得气”的状态。

关键词: 痛经, 子宫收缩强度, 不同针刺刺激量, 得气, 前列腺素EP2, 前列腺素F, 大鼠

Abstract: Objective To evaluate the effects of acupuncture with different stimulation intensity at Sanyinjiao (SP6) on uterine contraction and expression of endocrine-related molecular receptor gene in rats with dysmenorrhea of cold coagulation pattern and to explore whether there is association between “deqi (qi arrival)” and stimulus intensity. Methods Diestrus female rats were randomly divided into 4 groups, including saline control group, model control group, deqi group (with strong stimulus) and No deqi group (with weak stimulus). The levels of uterine contraction was recorded during 20 mins acupuncture treatment. The expression of PGF2α mRNA was measured. Results Compared with the model control group, the uterine contraction wave number and uterine activity were significantly reduced in deqi group. The expressions of PGF mRNA in both deqi and No deqi group were also reduced compared with model group. Conclusion Our finding indicates that using thick needles, deep insertion with hand-manipulation seems more effective and could achieve desired level of “deqi” in dysmenorrhea rats.

Key words: dysmenorrhea, uterine contraction, different acupuncture stimuli, deqi, PGEP22, PGE, rats

中图分类号: 

  • R245.3