主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

北京中医药大学学报 ›› 2015, Vol. 38 ›› Issue (8): 529-531.doi: 10.3969/j.issn.1006-2157.2015.08.005

• 科技之窗 • 上一篇    下一篇

寻常型银屑病患者皮损中IL-22与IL-22R1、STAT3、c-myc的mRNA表达及关系研究*

陈曦, 李玲玲, 张润田, 夏梦, 董玢, 段行武#   

  1. 北京中医药大学东直门医院 北京 100700
  • 收稿日期:2015-02-28 出版日期:2015-08-30 发布日期:2015-08-30
  • 通讯作者: 段行武,男,博士,教授,博士生导师,研究方向:中医皮肤病学,E-mail:xwduan@sina.com
  • 作者简介:陈曦,女,在读博士生
  • 基金资助:
    国家自然科学基金资助项目(No. 81273755),国家青年自然科学基金项目(No. 81403404)

The mRNA expressions of ILL-22, IL-22R1, STAT3 and c-myc in skin lesions of patients with psoriasis vulgaris and their correlations*

CHEN Xi, LI Ling-ling, ZHANG Run-tian, XIA Meng, DONG Bin, DUAN Xing-wu#   

  1. Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700
  • Received:2015-02-28 Online:2015-08-30 Published:2015-08-30

摘要: 目的 探究白细胞介素-22(IL-22)及IL-22R1、信号转导和转录激活因子-3(STAT3)、原癌基因(c-myc)在银屑病表皮异常增殖中的作用。方法 采用逆转录聚合酶链反应(RT-PCR)技术对23例寻常型银屑病患者皮损和11例正常人皮肤中IL-22、IL-22R1、STAT3、c-myc的mRNA水平进行检测。结果 IL-22、IL-22R1、STAT3、c-myc的mRNA在寻常型银屑病皮损中和正常人皮肤中均呈阳性表达;但在寻常型银屑病皮损中的表达水平较正常人组明显升高,差异有统计学意义(P<0.01)。IL-22的mRNA水平与IL-22R1、STAT3的mRNA水平呈正相关(P<0.05);STAT3的mRNA水平与c-myc的mRNA水平呈正相关(P<0.05)。结论 IL-22可能诱导STAT3活化导致银屑病角质形成细胞(KC)过度增殖,这为银屑病治疗提供了新的靶点。

关键词: 寻常型银屑病, 白细胞介素-22, 信号转导和转录激活因子-3, 原癌基因

Abstract: Objective To investigate the roles of IL-22, IL-22R1, Stat3 and c-myc mRNA expressions in abnormal proliferation of keratinocytes in psoriasis vulgaris. Methods Biopsies of skin lesions were conducted in 23 patients with psoriasis vulgaris and the skin samples of 11 normal control subjects. Levels of IL-22,IL-22R1, Stat3 and c-myc mRNA expressions were measured with reverse transcription-polymerase chain reaction (RT-PCR). Results IL-22, IL-22R1, Stat3 and c-myc mRNA were positively expressed in the psoriatic skin lesions as well as the normal controls. But the levels in the psoriatic skin lesions were significantly higher than those in the normal controls (P<0.01). There were positive correlations between the expression of IL-22 mRNA and that of IL-22R1/Stat3 mRNA (P<0.05). There were also positive correlations between the expression of Stat3 mRNA and that of c-myc mRNA (P<0.05). Conclusion The activation of Stat3 induced by IL-22 may lead to excessive proliferation of keratinocytes in psoriasis vulgaris, which could provide a new perspective in the treatment.

Key words: psoriasis vulgaris, Interleukin-22, IL-22R1, Stat3, c-myc

中图分类号: 

  • R275.9