主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

北京中医药大学学报 ›› 2015, Vol. 38 ›› Issue (8): 539-545.doi: 10.3969/j.issn.1006-2157.2015.08.008

• 中药化学 • 上一篇    下一篇

基于药物体系的开心散药物制备物质量表征关联分析研究*

李焕娟1, 张璐1, 权建野1, 杨书娟1, 杨元1, 陈唯1, 孙道涵1, 冯朵1, 姜艳艳1,2, 石任兵1,2#   

  1. 1 北京中医药大学中药学院 国家中医药管理局中药经典名方有效物质发现重点实验室 北京 100102;
    2 北京市教委中药质量控制技术工程中心
  • 收稿日期:2014-12-30 出版日期:2015-08-30 发布日期:2015-08-30
  • 通讯作者: 石任兵,男,博士,教授,博士生导师,研究方向:中药(复方)有效物质基础研究与药物创新,E-mail:shirb@126.com
  • 作者简介:李焕娟,女,在读硕士生
  • 基金资助:
    国家自然科学基金资助项目(No.81102776),十二五国家科技支撑计划项目(No.2012BA129B06),北京中医药大学创新团队项目(No.2011-CXTD-12)

Quality representation and correlation analysis of medicinal
preparation of Kaixin San based on drug system*

LI Huan-juan1, ZHANG Lu1, QUAN Jian-ye1, YANG Shu-juan1, YANG Yuan1, CHEN Wei1, SUN Dao-han1, FENG Duo1, JIANG Yan-yan1, SHI Ren-bing1,2#   

  1. 1 Key Unit of Exploring Effective Substances of Classical and Famous Formulas of State Administration of Traditional Chinese Medicine, School of Materia Medica, Beijing University of Chinese Medicine, Beijing 100102;
    2 Quality Control Technology and Engineering Center of Chinese Medicine, Beijing Municipal Commission of Education
  • Received:2014-12-30 Online:2015-08-30 Published:2015-08-30

摘要: 目的 确定影响开心散药物质量的关键因素,优选出开心散的最佳药物制备物形式,为创制开心散优质抗痴呆药物奠定基础。方法 采用所建立的HPLC分析法测定开心散制备物有效指标性成分的含量。基于制备物主要有效指标性成分的含量、含量相对比值、相对制备量以及出膏率和抗痴呆药效对开心散药物制备物的质量进行系统表征,并将质量表征结果进行关联分析。结果 基于药物体系所建立的开心散有效指标性成分的含量测定方法操作简便、准确、 重复性好, 可用于开心散药物制备物质量表征研究。开心散3种药物制备物形式均表现出抗痴呆作用,其作用大小为: 富集物>稀醇提取物>水提取物。通过开心散药物制备物药学-药效质量表征关联分析可知,开心散富集物中有效指标性成分的含量最高、比例独特、出膏率低、药物用量最小,改善学习记忆作用最强,为开心散抗痴呆药物最佳制备物形式。结论 基于开心散的基本组成及其制备得率,探寻药物成分的最适含量及其相对比值,是提升药物药效的关键因素。

关键词: 药物体系, 开心散药物制备物, 质量表征, 关联分析

Abstract: Objective To determine the key factors affecting the quality of Kaixin San Heart-opening powder and the best preparation forms of Kaixin San,laying the groundwork of anti-dementia drug development from Kaixin San. Methods The content of effective index components of medicinal-preparation of Kaixin San was detected by using HPLC. The quality of medicinal preparation from Kaixin San were characterized based on the content,relative quantity,relative ratio of content, yield rate and anti-dementia effect,and the characterized Results were given correlation analysis. Results The method of the determination of effective index components from Kaixin San is easy to handle,accurate and highly reproducible. The effect of anti-dementia of three medicinal preparations from Kaixin San was listed in the following descending order of enrichment, ethanol extract, and water extract. The content of effective index components is higher with unique proportion, lower extraction rate,lower dosage and higher antibacterial effect,which is the best form in medicinal-preparation application of Kaixin San. Conclusion The key to the improvment of efficacy is to explore the fittest content and relative ratio of medicinal components based on the basic composition of Kaixin San and yield rate.

Key words: drug system, medicinal-preparation of Kaixin San, quality characteristics, correlation analysis

中图分类号: 

  • R284.1