主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

北京中医药大学学报 ›› 2015, Vol. 38 ›› Issue (8): 556-560.doi: 10.3969/j.issn.1006-2157.2015.08.011

• 中药化学 • 上一篇    下一篇

注射用丹参(冻干)中糖类成分的两种色谱测定方法比较*

刘静1, 韩春霞1, 姜岩1, 王钢力1, 林瑞超2#, 戴忠1, 马双成1   

  1. 1 中国食品药品检定研究院中药所 北京 100050;
    2 北京中医药大学中药学院
  • 收稿日期:2014-12-04 出版日期:2015-08-30 发布日期:2015-08-30
  • 通讯作者: 林瑞超, 男, 博士, 教授, 博士生导师, 研究方向: 中药化学和中药分析, E-mail: linrch307@sina.com
  • 作者简介:刘静, 女, 博士, 副研究员
  • 基金资助:
    中国博士后科学基金第50批面上资助项目(No. 2011M500494)

Comparison of two chromatographic methods for the determination of sugars in Danshen Injection (lyophilized powder)*

LIU Jing1, HAN Chun-xia1, JIANG Yan1, WANG Gang-li1, LIN Rui-chao2#, DAI Zhong1, MA Shuang-cheng1   

  1. 1 Institute for Control of Chinese Traditional Medicine and Ethnic Medicine, National Institutes for Food and Drug Control, Beijing 100050;
    2 School of Chinese Materia Medica, Beijing University of Chinese Medicine
  • Received:2014-12-04 Online:2015-08-30 Published:2015-08-30

摘要: 目的 通过对糖类成分的两种色谱方法进行比较,选择一种准确、方便、快速测定注射用丹参(冻干)中糖类成分的方法。 方法 采用高效液相-蒸发光散射法 (HPLC-ELSD) , Alltech PrevailTM Carbohydrate ES (4.6 mm × 250 mm, 5 μm) 色谱柱,乙腈-水 (76∶24) 流动相等度洗脱,ELSD检测器。采用离子色谱法 (IC),CarboPac PA20 (3 mm×150 mm, S/N 003654 ) 阴离子交换分析色谱柱,CarboPac PA20 (3 mm×50 mm, S/N 003405 ) 保护柱,以氢氧化钠溶液(7 mmol/L)洗脱,脉冲安培检测器。以上两种色谱方法分别对注射用丹参(冻干)中糖类成分进行测定。 结果 在上述色谱条件下,HPLC-ELSD法可同时测定果糖和葡萄糖2种糖类成分,方法的线性关系、精密度、重复性及回收率等均符合要求;IC法可同时测定阿拉伯糖、半乳糖、葡萄糖、蔗糖、甘露糖和果糖6种糖类成分,方法经考察准确可靠。两种色谱方法分别测定的果糖和葡萄糖结果基本一致。结论 离子色谱法更适合于注射用丹参(冻干)中糖类成分的测定。

关键词: 注射用丹参(冻干), 糖类成分, 蒸发光散射检测, 离子色谱

Abstract: Objective To compare two chromatographic Methods and then to select an accurate, convenient and rapid method for the determination of sugars in Danshen Injection (lyophilized powder). Methods Firstly, HPLC-ELSD method was applied on an Alltech PrevailTM Carbohydrate ES (4.6 mm × 250 mm, 5 μm) column, and acetonitrile-water (76∶24) was used as the mobile phase on an isocratic elution with ELSD as the detector. Apart from that, IC method was adopted on a CarboPac PA20 (150 mm × 3 mm, S/N 003654 ) anion exchange column with the CarboPac PA20 ( 50 × 3 mm, S/N 003405) column as the guard column, and sodium hydrate solution (7 mmol/L) was used as eluent with the pulsed amperometric detector. To sum up, HPLC-ELSD and IC Methods described above were applied to determine the sugars in Danshen Injection (lyophilized powder) respectively. Results Under the chromatographic conditions mentioned above, HPLC-ELSD method could simultaneously determine the contents of fructose and glucose. Linear relationship, precision, repeatability and recovery rate of the method were all qualified; IC method could simultaneously determine arabinose, galactose, glucose, sucrose, mannose as well as fructose, and the method proved to be accurate and reliable. The determination Results of fructose and glucose by both Methods were basically the same. Conclusion IC method seems to be a better choice to determine the monosaccharides and disaccharide in Danshen Injection (lyophilized powder).

Key words: Danshen Injection (lyophilized powder), sugars, evaporative laser light detection, ion chromatography

中图分类号: 

  • R284.1