主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

北京中医药大学学报 ›› 2015, Vol. 38 ›› Issue (10): 682-685.doi: 10.3969/j.issn.1006-2157.2015.10.008

• 博士之光 • 上一篇    下一篇

基于蛋白酶激活受体2表达研究药对防风-乌梅抗过敏的配伍机制

吴贤波1,朱海燕1#,李向1,徐玉刚,1,金沈锐2,,王明谊2   

  1. 1.延边中医医院延吉市中医医院 吉林 133000;
    2.成都中医药大学
  • 收稿日期:2015-03-16 出版日期:2015-10-30 发布日期:2015-10-30
  • 通讯作者: 朱海燕,女,博士,主治医师,主要研究方向:方剂配伍规律研究,E-mail:zhuhaiyan19791@126.com
  • 作者简介:吴贤波,男,博士,副教授,硕士生导师
  • 基金资助:
    国家自然科学基金项目(No.81102677)

Anti-allergy mechanism of fangfeng-wumei pair based on expressions of PAR-2*

WU Xianbo1, ZHU Haiyan1#, LI Xiang1, XU Yugang1, JIN Shenrui2, WANG Mingyi2   

  1. 1.Yanbian Hospital of Traditional Chinese Medicine, Yanji city, Jilin 133000;
    2.Chengdu University of Traditional Chinese Medicine
  • Received:2015-03-16 Online:2015-10-30 Published:2015-10-30

摘要: 目的 观察防风和乌梅配伍前后对肥大细胞脱颗粒以及蛋白酶激活受体2(PAR-2)表达的影响,为药对防风-乌梅抗过敏的研究提供实验依据。方法 体外采用胰蛋白酶刺激小鼠肥大细胞株P815方法建立肥大细胞脱颗粒模型,运用RT-PCR、Western blotting、ELISA等技术,观察防风-乌梅配伍前后对肥大细胞分泌组胺、PAR-2 mRNA和蛋白表达的影响。结果 防风-乌梅配伍后在体外抑制肥大细胞分泌组胺、PAR-2表达的作用明显增强,与细胞模型组比较有统计学差异(P<0.05或P<0.01)。结论 防风-乌梅配伍后抑制脱颗粒作用明显增强,下调肥大细胞PAR-2表达可能是药对防风-乌梅抗过敏的配伍机制之一。

关键词: 防风, 乌梅, 药对, 蛋白酶激活受体2

Abstract: Objective By observing the effects fangfeng-wumei pair on degranulation of mast cells and PAR-2 expression before and after the pairing to provide experimental basis for the research on its anti-allergy mechanism. Methods A mast cell degranulation mouse model was established by stimulating mouse mast cell line P815 with trysin in vitro, and the levels of histamine as well as PAR-2mRNA and protein expression of P815 cells affected by fangfeng-wumei pair were measured using RT-PCR, Western blotting and ELISA. Results Fangfeng-wumei pair in vitro significantly inhibited the secretion of histamine and the expression of PAR-2 by mast cells in comparison to cell model group (P <0.05 or P <0.01). Conclusion The results demonstrated that inhibition of degranulation of mast cells was significantly enhanced by fangfeng-wumei pair, and that the expression of PAR-2 by mast cells might be involved in the anti-allergy mechanism of fangfeng-wumei pair.

Key words: Fangfeng, Wumei, medicinal pair, PAR-2

中图分类号: 

  • R285.5