主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

北京中医药大学学报 ›› 2016, Vol. 39 ›› Issue (1): 16-20.doi: 10.3969/j.issn.1006-2157.2016.01.003

• 科技之窗 • 上一篇    下一篇

糖肾清2号方对2型糖尿病模型小鼠肾功能的影响*

张继胜1, 商学征1, 白羽1, 王亚南1, 马卫国1, 刘慧1, 王康1, 陈祎2, 陆妍3, 沈志明1, 孟凤仙1#   

  1. 1 北京中医药大学东方医院 北京 100078;
    2 北京中医药大学第三附属医院;
    3 中国中医科学院西苑医院
  • 收稿日期:2015-05-27 出版日期:2016-01-30 发布日期:2016-01-30
  • 通讯作者: 孟凤仙,女,教授,主任医师,博士生导师,E-mail:mfx0823@163.com
  • 作者简介:张继胜,男,主治医师
  • 基金资助:
    *国家自然科学基金资助项目(No.81273886)

Protective effect of Tangshenqing Formula II on renal function of type 2 diabetic mice *

ZHANG Jisheng1, SHANG Xuezheng1, BAI Yu1, WANG Yanan1, MA Weiguo1, LIU Hui1, WANG Kang1, CHEN Yi2, LU Yan3, SHEN Zhiming1, MENG Fengxian1#   

  1. 1 Dongfang Hospital, Beijing University of Chinese Medicine,Beijing 100078;
    2 Third Affiliated Hospital, Beijing University of Chinese Medicine;
    3 Xiyuan Hospital of China Academy of Chinese Medical Sciences
  • Received:2015-05-27 Online:2016-01-30 Published:2016-01-30

摘要: 目的 观察糖肾清2号方对2型糖尿病模型小鼠肾功能的保护作用。方法 采用自发性2型糖尿病模型KK-Ay小鼠,予高脂饲料诱导3周后,按血糖及体重水平随机分为模型组、缬沙坦组、糖肾清2号方大、中、小剂量组,以C57BL/6J小鼠设为正常组,每组各6只,连续干预治疗12周。分别在0、4、8、12周检测各组小鼠空腹血糖、尿微量白蛋白(Alb)、尿肌酐(Cr)、尿微量白蛋白/尿肌酐(ACR)及24 h尿蛋白,药物干预12周后实验结束,留取血清标本,全自动生化分析仪检测甘油三酯(TG)、总胆固醇(TC)、血清肌酐(Scr)、尿素氮(BUN)水平,酶联免疫吸附试验(ELISA)法检测血清β2微球蛋白(β2-MG)水平变化。结果 与正常组比较,模型组小鼠24 h尿蛋白、ACR、Scr、TG、TC水平显著升高(P<0.05,P<0.01),BUN、β2-MG及肾重/体重变化不显著(P>0.05)。与模型组比较,缬沙坦组及糖肾清2号方大、中、小剂量组小鼠Scr、TG、ACR水平明显下降(P<0.05,P<0.01);24 h尿蛋白、TC、空腹血糖等指标有不同程度下降。结论 糖肾清2号方可明显降低KK-Ay小鼠空腹血糖、Scr、TG水平,减轻糖尿病肾病(DN)肾损害,延缓DN发病进程。

关键词: 糖尿病肾病, 肾功能, 糖肾清2号方, 小鼠

Abstract: Objective To observe the protective effect of Tangshenqing Formula II (TSQF)on the renal function of mice with type 2 diabetes mellitus (DM). Methods KK-Ay mice, a spontaneous DM model, were fed with fat-enriched diet for three weeks. Based on their serum glucose (Glu) and body weight (BW), the mice were divided into model group, valsartan group, high, middle, and low dose of TSQF (high-, mid-, low- dose) group (n=6 each) and were treated for consecutive 12 weeks. And six C57BL/6J mice were included into control group. At the timepoint of Week 0, 4, 8, 12, Glu, urine albumin (Alb), urine creatine (Cr), Alb/Cr(ACR) and 24-h urine protein (pr/24 h) were detected. At the end of Week 12, all the mice were sacrificed and their serum samples were collected. And the serum levels of TG, TC, Scr, BUN and β2-MG were measured. Results Compared with the control group, the levels of pr/24 h, ACR, Scr, TG and TC in the model group significantly increased (P<0.05 or P<0.01), While BUN, β2-MG and ratio of kidney weight to body weight showed no statistcal differences(P>0.05). Compared to the model group, the levels of Scr, TG and ACR in valsartan group and three TSQF groups significantly decreased (P<0.05 or P<0.01), and those of TC, Glu, and pr/24 h decreased at different degree. Conclusion TSQF could reduce the serum levels of Glu, Scr and TG of kk-Ay mice, and alleviate the kidney injuries to reduce the progression of diabetic nephropathy.

Key words: diabetic nephropathy, renal function, Tangshenqing Formula II, mice

中图分类号: 

  • R285.5