主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

北京中医药大学学报 ›› 2016, Vol. 39 ›› Issue (2): 122-127.doi: 10.3969/j.issn.1006-2157.2016.02.007

• 科技之窗 • 上一篇    下一篇

Toll样受体8基因rs3764880多态性与汉族男性缺血性中风的关联性研究*

古联1, 周金英2, 谭金晶3, 梁宝云1, 严雁1, 陈清1, 谢娟娟3, 唐农2#   

  1. 1 广西中医药大学第一附属医院脑病科 广西 530023;
    2 广西中医药大学;
    3 广西中医药大学第一附属医院重症医学科
  • 收稿日期:2015-08-17 出版日期:2016-02-28 发布日期:2016-02-28
  • 通讯作者: 唐农,男,教授,主任医师,E-mail:tangnong2012@163.com
  • 作者简介:古联,男,教授,主任医师
  • 基金资助:
    *国家自然科学基金资助项目(No.81260594,81473670), 广西省自然科学基金项目(No. 2013GXNSFAA019145), 广西中医药大学第一附属医院青年科学基金课题(No. GZYQJ2014007), 广西高等学校优秀中青年骨干教师培养工程资助成果

Toll-like receptor-8 gene rs3764880 polymorphism and ischemic stroke risk in patients of Han Chinese males*

GU Lian1, ZHOU Jinying2, TAN Jinjing3, LIANG Baoyun1, YAN yan1, CHEN Qing1, XIE Juanjuan3, TANG Nong2#   

  1. 1 Department of Neurology, First Affiliated Hospital, Guangxi University of Traditional Chinese Medicine, Guangxi 530023;
    2 Guangxi University of Traditional Chinese Medicine;
    3 Department of Intensive Medicine, the First Affiliated Hospital, Guangxi University of Traditional Chinese Medicine
  • Received:2015-08-17 Online:2016-02-28 Published:2016-02-28

摘要: 目的 探讨Toll样受体8 (TLR8) 基因rs3764880遗传多态性与缺血性中风风痰瘀阻证、气虚血瘀证的易感性及相关数量性状的关联。方法 纳入缺血性中风风痰瘀阻证患者311例、气虚血瘀证284例,对照组605例。用real-time PCR技术检测外周血TLR8基因表达水平,Sequenom MassARRAY platform进行基因分型,ELISA法测定炎性细胞因子血清水平。以PLINK、SPSS 16.0软件进行统计分析。结果 TLR8基因rs3764880多态性与中国汉族男性中风风痰瘀阻证易感性的关联有统计学意义(等位基因模型P<0.05)。在气虚血瘀证患者中,携带GG基因型者的血清白细胞介素8(IL-8)水平较携带AA+GG基因型者高(P<0.05);携带GG+AG基因型者的肿瘤坏死因子α(TNF-α)、IL-8血清水平高于携带AA基因型者(P<0.05)。rs3764880多态性与男性气虚血瘀证患者的胆固醇(TC)(加性模型:β=-0.03,Padj=0.001)、低密度脂蛋白(LDL)水平(加性模型:βb=-0.25,Padj=0.018)显著关联。结论 TLR8基因rs3764880多态性可能影响中国汉族男性缺血性中风风痰瘀阻证发生,并影响气虚血瘀证的炎性反应和脂质代谢。

关键词: 缺血性中风, 中医证型, Toll样受体8, 单核苷酸多态性, 炎性细胞因子

Abstract: Objective To explore the association between Toll-like receptor-8(TLR8) genetic polymorphisms and susceptibility to ischemic stroke (IS) in patients of different traditional Chinese medicine (TCM) patterns. Methods 311 IS patients of wind-phlegm stasis-obstruction pattern, 284 IS patients of qi-deficiency blood-stasis pattern and 605 controls were recruited. The real-time PCR technology was used to detect TLR8 gene expression levels. TLR8 rs3764880 single nucleotide polymorphism(SNPs) were genotyped using the Sequenom Mass ARRAY platform. Levels of inflammatory cytokines were measured by using ELISA. Statistical analyses were carried out by using PLINK and SPSS 16.0 software. Results TLR8 gene variant rs3764880 polymorphism was significantly associated with wind-phlegm stasis-obstruction pattern in IS males patients in the allele model (P<0.05). In IS male patients of qi-deficiency blood-stasis pattern , level of IL-8 was higher in the GG genotype carriers than the AA+AG genotype carriers (P<0.05). The GG+AG genotype carriers had higher levels of TNF-α and IL-8 than the AA genotype carriers(P<0.05) . TLR8 gene variant rs3764880 polymorphism was significantly associated with the levels of TC (βb=-0.03, Padj=0.001) and LDL (βb=-0.25, Padj=0.018) in additive model in qi-deficiency blood-stasis pattern in Han Chinese IS male patients. Conclusion TLR8 gene rs3764880 polymorphism might be associated with the susceptibility to IS in patients of wind-phlegm stasis-obstruction and affect inflammatory reaction and lipid metabolism in Han Chinese males of qi-deficiency blood-stasis pattern.

Key words: ischemic stroke, traditional Chinese medicine pattern, Toll-like receptor-8, single nucleotide polymorphism, inflammatory cytokines

中图分类号: 

  • R255.2