主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

北京中医药大学学报 ›› 2016, Vol. 39 ›› Issue (3): 241-246.doi: 10.3969/j.issn.1006-2157.2016.03.013

• 针灸 • 上一篇    下一篇

针刺对强迫游泳应激抑郁模型大鼠海马半胱氨酸天冬氨酸蛋白酶-3、c-Jun表达的影响*

郭郁1,包伍叶2,图娅1#,邬继红1,王瑜1,,,,,,张旭辉1,,许明敏1,于淼1,张春涛1,赵冰骢1,,   

  1. 1 北京中医药大学针灸推拿学院 北京 100029;
    2 内蒙古民族大学附属医院
  • 收稿日期:2015-10-06 出版日期:2016-03-30 发布日期:2016-03-30
  • 通讯作者: 图娅,女,博士,教授,主任医师,博士生导师,主要研究方向:针灸经典理论的现代研究,E-mail:Tuyab@263.net
  • 作者简介:郭郁,男,在读硕士生
  • 基金资助:
    *国家自然科学基金面上项目(No.81173334,81373729)

Effects of acupuncture on cysteine-containing aspartate-specific proteases 3 and c-Jun in the hippocampus of depression rats induced by force swimming stress*

GUO Yu1, BAO Wuye2, TU Ya1#,WU Jihong1,WANG Yu1, ZHANG Xuhui1, XU Mingmin1, YU Miao1, ZHANG Chuntao1, ZHAO Bingcong1   

  1. 1 School of Acupuncture-Moxibustion and Tuina,Beijing University of Chinese Medicine,Beijing 100029;
    2 Affiliated Hospital of Inner Mongolia University for the Nationalities
  • Received:2015-10-06 Online:2016-03-30 Published:2016-03-30

摘要: 目的 探讨针刺对海马神经元凋亡的干预作用及是否涉及c-Jun氨基末端激酶(c-Jun N-terminal Kinase,JNK)信号通路,以揭示针刺的相关抗抑郁机制。方法 将36只SD大鼠随机分成空白组、空白阻断剂组、模型组、模型阻断剂组、针刺组、针刺阻断剂组,阻断剂为JNK通路阻断剂SP600125,每组6只,采用强迫游泳应激结合孤养方法造模,针刺干预选取百会、印堂穴,每次20 min,每天1次,治疗2 d。采用蛋白免疫印迹法检测大鼠海马JNK信号通路的底物c-Jun蛋白表达水平;采用实时荧光定量PCR(RT-PCR)技术检测大鼠海马凋亡相关因子半胱氨酸天冬氨酸蛋白酶-3(Caspase-3)基因表达水平。结果 与空白组比较,Caspase-3基因表达、c-Jun蛋白表达,空白阻断剂组无显著差异(P>0.05),模型组显著升高(P<0.01);与模型组比较,模型阻断剂组、针刺组、针刺阻断剂组Caspase-3基因表达均显著降低(P<0.01),模型阻断剂组、针刺组、针刺阻断剂组c-Jun的蛋白表达显著降低(P<0.05、P<0.05、P<0.01)。结论 针刺干预治疗可能通过抑制大鼠海马JNK信号通路活性,降低c-Jun的蛋白水平,减少促凋亡蛋白Caspase-3基因表达,从而抑制海马神经元凋亡,这可能是针刺抗抑郁作用所涉及到的分子机制之一。

关键词: 针刺, 强迫游泳应激, 海马, 抑郁症, 半胱氨酸天冬氨酸蛋白酶-3, c-Jun, 阻断剂SP600125, 大鼠

Abstract: Objective To observe the interventional effect of acupuncture on apoptosis of hippocampal neuron of depression model rats, and the effect on the c-Jun N-terminal kinase(JNK)pathway, so as to reveal the mechanism of anti-depression of acupuncture. Methods Altogether 36 Sprague-Dawley rats were randomly divided into six groups (each n=6): control group, control+ inhibitor (SP600125, a JNK inhibitor ) group, model group, model + inhibitor group, acupuncture group and acupuncture + inhibitor group. Except the control group and control + inhibitor group, the rats in the other four groups were forced to swim and be bred solitarily to establish the rat depression model. Baihui (GV20) and Yintang (GV29), the acupoints selected in rats of acupuncture group and acupuncture + inhibitor group, were needled for 20 minutes, once daily for two days. Using Western blot to detect the protein expression of c-Jun, the substrate of JNK pathway, in hippocampus of all groups of rats, and using RT-PCR to examine the gene expression of apoptosis-related caspase-3 in hippocampus of rats. Results Compared with the control group, caspase-3 gene expression and c-Jun protein expression showed no significant difference in control + inhibitor group(P>0.05), but both were higher in those of model group(P<0.01). Compared with model group, the expression levels of caspase-3 were lower significantly in model+inhibitor group, acupuncture group and acupuncture + inhibitor group(P<0.01), and the protein expression levels of c-Jun were lower significantly in model+inhibitor group, acupuncture group and acupuncture + inhibitor group(P<0.05,P<0.05,P<0.01). Conclusion Apoptosis of hippocampal neurons may be inhibited via the interventional effect of acupuncture on JNK pathway activities which down-regulate c-Jun protein expression and apoptosis-related caspase-3 gene expression. It may be one of the potential molecular mechanisms of antidepressant effect of acupuncture.

Key words: acupuncture, forced swimming stress, hippocampus, depression, cysteine-containing aspartate-specific protease 3, c-Jun, JNK inhibitor SP600125, rats

中图分类号: 

  • R245.31+1