主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

北京中医药大学学报 ›› 2016, Vol. 39 ›› Issue (6): 480-488.doi: 10.3969/j.issn.1006-2157.2016.06.009

• 中药化学 • 上一篇    下一篇

基于酚类成分特征图谱的女贞子质量表征研究*

赵丽敏1,卢广英1,杨书娟1,许舒娅1,彭平1,李钢1,李焕娟1,冯朵1,阳长明3,石任兵1,2#   

  1. 1 北京中医药大学中药学院 国家中医药管理局中药经典名方有效物质发现重点实验室 北京 100102;
    2 北京市教委中药质量控制技术工程中心;
    3 国家食品药品监督管理局药品审评中心
  • 收稿日期:2016-01-05 出版日期:2016-06-30 发布日期:2016-06-30
  • 通讯作者: 石任兵,男,博士,教授,博士生导师,研究方向:中药(复方)有效物质基础研究与药物创新,E-mail:shirb@126.com
  • 作者简介:赵丽敏,女,在读硕士生
  • 基金资助:
    *十二五国家科技支撑计划项目(No.2012BAI29B06),北京中医药大学创新团队资助项目(No.2011-CXTD-12)

Quality representation and correlation analysis of Ligustrum lucidum Ait. based on characteristic spectrum of drug system*

ZHAO Limin1, LU Guangying1, YANG Shujuan1, XU Shuya1, PENG Ping1, LI Gang1, LI Huanjuan1, FENG Duo1,YANG Changming3, SHI Renbing1,2#   

  1. 1 Key Unit of Exploring Effective Substances of Classical and Famous Formulas of State Administration of Traditional Chinese Medicine, School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102;
    2 Quality Control Technology and Engineering Center of Chinese Medicine, Beijing Municipal Commission of Education;
    3 Center for Drug Evaluation, China Food and Drug Administration
  • Received:2016-01-05 Online:2016-06-30 Published:2016-06-30

摘要: 目的 建立基于酚类成分特征图谱的女贞子质量表征及关联分析方法,以精确全面地评价女贞子饮片质量。方法 采用所建立的反相高效液相色谱(HPLC-PDA)法表征女贞子药材的特征图谱质量,基于特征图谱中特征峰的数量及所属化学类型对14批女贞子饮片进行质的表征。基于特征图谱中特征指标性成分红景天苷、酪醇、特女贞苷、槲皮素的含量及环烯醚萜类(以特女贞苷表征)、黄酮类(以槲皮素表征)的含量对14批女贞子饮片进行量的表征,并基于基准饮片将14批女贞子饮片质与量的表征结果分别进行关联性分析。结果 以批号S10为基准,女贞子饮片特征图谱中含有酚类特征共有峰11个,其中苯乙醇苷类成分特征峰2个、环烯醚萜类成分特征峰5个、黄酮类成分特征峰4个,14批饮片色谱图中均含有此11个特征峰。样品S14、S12、S5、S8、S9、S6、S11中特征指标性成分含量总体较高,样品S14、S12、S5、S8、S9与基准样品S10关联性最高,综合评价得出样品S5、S8、S9、S12、S14质量优良度居前。结论 基于特征图谱的质与量及其关联性的系统表征所建立的基于药物体系的女贞子特征图谱质量表征关联分析评价模式,关注了女贞子质量整体关联性与应用有效性,能够有效精准评价其质量。

关键词: 女贞子, 特征图谱, 质量表征, 红景天苷, 酪醇, 特女贞苷, 槲皮素

Abstract: Objective To establish quality representation and correlation analysis method of Ligustrum lucidum Ait. (Glossy Privet Fruit, Nüzhenzi)based on characteristic spectrum of drug system, so as to evaluate the quality of Nüzhenzi decoction pieces accurately and comprehensively. Methods Using established HPLC-PDA method to represent the quality of Nüzhenzi characteristic spectrum of drug system. Then the qualitative representation of 14 batches of Nüzhenzi decoction pieces was based on the quantity and type of characteristic peaks of specific chromatograms, the quantitative representation of 14 batches of Nüzhenzi pieces was based on the content of four characteristic index components including salidroside, tyrosol, specnuezhenide and quercetin and the sum of iridoids (using specnuezhenide for representation) and flavonoids (using quercetin for representation), then correlation analysis was based on the representation results of quantity and quality respectively in 14 batches of Nüzhenzi pieces referenced to the benchmark decoction piece. Results Taking Lot. S10 as the benchmark, in Nüzhenzi HPLC specific chromatogram there were 11 characteristic peaks which were common in all batches, including two benzene glycosides peaks ,five iridoids peaks,four flavonoids peaks. The contents of effective index components in batch S14, S12,S5,S8,S9,S6 and S11 were higher, with the highest correlation in S14, S12, S5, S8, S9 and S10, so the quality of S5, S8, S9, S12, and S14 were superior to other batches. Conclusion The evaluation model of quality representation and correlation analysis of Nüzhenzi specific chromatogram concerning on correlation of the overall quality and the effectiveness of the application, can effectively and accurately evaluate the quality of Nüzhenzi.

Key words: Nüzhenzi, characteristic spectrum, quality representation, salidroside, tyrosol, specnuezhenide, quercetin

中图分类号: 

  • R284.1