主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

北京中医药大学学报 ›› 2016, Vol. 39 ›› Issue (7): 580-585.doi: 10.3969/j.issn.1006-2157.2016.07.011

• 科技之窗 • 上一篇    下一篇

白花蛇舌草对巨噬细胞Cxcl1基因表达的影响及机制的初步研究*

岳晓宁,施扬,臧师竹#   

  1. 大连医科大学生物技术系 辽宁 116044
  • 收稿日期:2016-02-23 出版日期:2016-07-30 发布日期:2016-07-30
  • 通讯作者: 臧师竹,女,副教授,研究方向:2型糖尿病预防,E-mail: szdl2001@163.com
  • 作者简介:岳晓宁,男,在读本科生
  • 基金资助:
    *辽宁省教育厅自然科学一般项目(No.L2011159),2014年大连医科大学大学生创新创业训练计划项目(No.201410161000025)

Effect and mechanism of oldenlandia on Cxcl1 gene expression in macrophages*

YUE Xiaoning, SHI Yang, ZANG Shizhu#   

  1. Biotechnology Department, Dalian Medical University, Liaoning 116044
  • Received:2016-02-23 Online:2016-07-30 Published:2016-07-30

摘要: 目的 通过研究白花蛇舌草对巨噬细胞细胞因子表达的影响及Toll样受体4(TLR-4)炎性信号转导通路的影响,探索白花蛇舌草的免疫调节作用,为新药研发提供实验和理论基础。方法 用白花蛇舌草水提液作用小鼠腹腔巨噬细胞,RT-PCR法检测3种常见细胞因子白细胞介素6(IL-6)、单核细胞趋化因子1(MCP-1)、Cxcl1的表达。然后用Autodock 软件分析白花蛇舌草主要成分与TLR-4的相互作用,得出白花蛇舌草的主要活性物质对于TLR介导的信号通路的作用机理,从而得出白花蛇舌草对巨噬细胞炎症因子释放以及吞噬功能的影响。结果 白花蛇舌草能上调Cxcl1基因的表达,其主要成分豆甾醇-β-D-葡萄糖苷、反式4-甲基肉桂醇、车叶草苷酸可以与TLR-4受体结合。结论 白花蛇舌草可以激活TLR-4信号通路,从而影响Cxcl1的表达及巨噬细胞功能,因此推测白花蛇舌草有调控免疫的作用。

关键词: 白花蛇舌草, 巨噬细胞, Cxcl1基因, Toll样受体4

Abstract: Objective To explore the immunoregulatory effect of Oldenlandia (Hedyotis diffusa Villd.), by investigating on the expression of macrophage cytokines and on TLR-4 inflammatory signal transduction pathway, aiming to propose a new experimental and theoretical evidence for the use of oldenlandia.Methods Three common cytokines, such as IL-6, MCP-1 and Cxcl1 in macrophage cells were detected by using RT-PCR after treatment with oldenlandia solution. Autodock software was used to analyze the correlation between the main components and TLR-4 so as to explore the mechanism of expression changes of the cytokines. Results Oldenlandia solution could upregulate the expression of Cxcl1, which might depend on the combination of TLR4 and stigmasterol-beta-D-glucoside, trans-4-methoxycinnamyl alcohol, and asperulosidic acid. Conclusion Oldenlandia could activate TLR-4 signal pathway and thus affect the expression of Cxcl1 and function of macrophage. It can be inferred that oldenlandia could regulate immunity, which could serve as the theoretical rationale for the development of new drugs in the laboratory.

Key words: Baihuashe Shecao (Hedyotis diffusa Villd.), macrophage, Cxcl1 gene, Toll like receptor 4

中图分类号: 

  • R285.5