主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

北京中医药大学学报 ›› 2016, Vol. 39 ›› Issue (10): 833-838.doi: 10.3969/j.issn.1006-2157.2016.10.010

• 科技之窗 • 上一篇    下一篇

温润辛金培本中药复方对变应性鼻炎豚鼠的影响*

续艳1,孔德明1,楚慧伦1,杨颖1,阎玥2,李友林2,孙文燕1#   

  1. 1 北京中医药大学中药学院 北京 100102;
    2 中日友好医院肺病慢性咳喘国家中医药管理局重点研究室
  • 收稿日期:2016-05-10 出版日期:2016-10-30 发布日期:2016-10-30
  • 通讯作者: 孙文燕,男,博士,副教授,硕士生导师,主要研究方向:中药防治免疫性疾病的药理学研究及中药毒理学研究,E-mail:sunwy@bucm.edu.cn
  • 作者简介:续艳,女,在读硕士生
  • 基金资助:
    *北京市科学技术委员会“十病十药”研发专项课题(No.Z141100002214018),北京中医药大学自主选题(在读研究生项目)(No.2016-JYB-XS106)

Effects of Chinese medicine compound of warming and moistening lung with tonifying spleen on guinea pigs with allergic rhinitis*

XU Yan1,KONG Deming1,CHU Huilun1,YANG Ying1,YAN Yue2,LI Youlin 2 ,SUN Wenyan1#   

  1. 1 School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102;
    2 Key Institute of State Administration of Traditional Chinese Medicine of Pneumonupathy Chronic Cough and Dyspnea, China-Japan Friendship Hospital
  • Received:2016-05-10 Online:2016-10-30 Published:2016-10-30

摘要: 目的 研究温润辛金培本中药复方对变应性鼻炎(AR)豚鼠行为学、血清免疫球蛋白E(IgE)、白细胞介素-6(IL-6)以及鼻黏膜组织病理学的影响。方法 SPF级豚鼠,雌雄各半,随机分为正常组,模型组,鼻炎康组(0.41 g/kg),氯雷他定组(0.91 mg/kg),温润辛金培本中药复方高剂量组(3.04 g/kg)、中剂量组(1.52 g/kg)、低剂量组(0.76 g/kg)。除正常组外,其余豚鼠以10%的2,4-甲苯二异氰酸酯(TDI)橄榄油溶液双侧鼻孔滴鼻(每侧5 μL),每日1次,连续7 d。模型成功后,给药14 d,TDI改为隔日滴鼻1次。观察各组行为学并进行评分,酶联免疫吸附法(ELISA)检测血清IgE、IL-6的含量,光学显微镜下观察鼻黏膜组织病理学变化,并进行嗜酸性粒细胞(EOS)计数。结果 温润辛金培本中药复方中、高剂量组明显降低豚鼠行为学评分,与模型组比较P<0.01或P<0.05。温润辛金培本中药复方各剂量组均明显降低豚鼠血清IgE、IL-6水平,与模型组比较P<0.01或P<0.05。温润辛金培本中药复方中、高剂量组明显减轻鼻黏膜EOS浸润,与模型组比较P<0.01或P<0.05。温润辛金培本中药复方高剂量组黏膜上皮完好,未见炎性细胞浸润,与模型组比较P<0.05。结论 温润辛金培本中药复方可改善AR豚鼠行为学,降低豚鼠血清IgE、IL-6水平,减轻鼻黏膜病理变化。

关键词: 温润辛金培本中药复方, 变应性鼻炎, 行为学, 病理学, 免疫球蛋白E, 白细胞介素-6, 豚鼠

Abstract: Objective To study the effects of Chinese medicine compound of warming and moistening lung with tonifying spleen(hereinafter“TCM”) on behavior, serum IgE and IL-6, as well as nasal mucosa pathological changes of guinea pigs with allergic rhinitis. Methods SPF grade guinea pigs, half male and half female, were randomly divided into normal group, model group, Biyankang group (0.41 g/kg), loratadine group (0.91 mg/kg), TCM high-dose group (3.04 g/kg), mid-dose group(1.52 g/kg) and low-dose group(0.76 g/kg). Except the normal group, other groups were followed by bilateralnostrils intranasal inhalation once daily with 10% TDI olive oil solution(5 μL each nostril), 7 days in a row. After the model was established successfully, the guinea pigs were i.g. corresponding medication, with dripping TDI continuously for 14 days every other day. Nasal symptoms of all groups were observed and recorded. The serum contents of IgE and IL-6 were determined by ELISA, nasal mucosa pathological morphological changes were observed under light microscope, and number of eosinophils (EOS) were counted. Results Compared with the model group, scores of behavioral symptom were significantly lower in both TCM high- and mid- dose group (P<0.01 or P<0.05), and serum content of IgE and IL-6 significantly lower in all the TCM groups (P<0.01 or P<0.05). High-dose and mid-dose of TCM relieved EOS infiltration of nasal mucosa,compared with model group (P<0.01 or P<0.05), furthermore, in TCM high dose group, no exposure-related nasal lesions or no flammatory cell infiltration was found (P<0.05). Conclusion TCM could improve the behavior of guinea pigs with allergic rhinitis and reduce serum IgE and IL-6 levels, relieve the nasal mucosa pathological changes.

Key words: Chinese medicine compound of warming and moistening lung tonifying spleen, allergic rhinitis, behavior, pathology, IgE, IL-6, guinea pigs

中图分类号: 

  • R285.5