主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

北京中医药大学学报 ›› 2016, Vol. 39 ›› Issue (11): 949-954.doi: 10.3969/j.issn.1006-2157.2016.11.012

• 中药制剂 • 上一篇    下一篇

辅料因素对炮制蒙药诃子制草乌质量的影响

刘帅,李卫飞,李妍,许金凯,刘晓艳,李飞,杜红#   

  1. 北京中医药大学 中药学院 北京 100102
  • 收稿日期:2016-06-06 出版日期:2016-11-30 发布日期:2016-11-30
  • 通讯作者: 杜红,女,博士,副教授,硕士生导师,研究方向:中药炮制,E-mail:duhong@rip.163.com
  • 作者简介:刘帅,男,在读硕士生
  • 基金资助:
    国家自然科学基金资助项目(No.81102815),北京市自然科学基金项目(No.7162120),北京中医药大学研究生自主课题(No.2016-JYB-XS069)

Influence of Fructus Chebulae water solution on quality of Mongolian medicine Aconiti Kusnezoffii Radix Praeparata

LIU Shuai, LI Weifei, LI Yan, XU Jinkai, LIU Xiaoyan, LI Fei, DU Hong#   

  1. School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102
  • Received:2016-06-06 Online:2016-11-30 Published:2016-11-30

摘要: 目的 探讨辅料因素对蒙药诃子制草乌质量的影响,阐明辅料发挥炮制减毒作用的原理,优选出最佳炮制工艺。方法 煎煮制备不同比例的诃子汤作为炮制辅料,分别测定其pH值、总鞣质含量。并用不同比例的诃子汤分别炮制草乌,采用反相高效液相色谱法检测各炮制品中代表毒效成分的6种生物碱含量。结果 随诃子用量增加,诃子汤中总鞣质含量不断升高;诃子汤pH值先减小,后基本维持稳定;诃子制草乌中6种生物碱总量先逐渐升高后变化不规律。其中草乌、诃子比例为2∶1时,诃子制草乌中生物碱含量达到最低,该比例与1986年《内蒙古蒙药材标准》中对诃子、草乌炮制用量的比例一致。结论 诃子用量及炮制辅料是否新鲜均会影响诃子制草乌中生物碱含量大小,《内蒙古蒙药材标准》对诃子、草乌炮制用量比2∶1具有一定的科学性,建议继续沿用。

关键词: 诃子制草乌, 炮制, 辅料, 生物碱含量, pH值, 鞣质

Abstract: Objective To study the influence of different concentration of Fructus Chebulae (Hezi) water solution on the quality of Aconiti Kusnezoffii Radix Praeparata (Zhicaowu), so as to optimize the processing technology and to find out the principle of detoxification of Hezi in processing. Methods Taking Hezi water solution with different concentration as the excipients, pH and content of total tannins were determined. Then content of six alkaloids, indicator of toxic effect, in Zhicaowu were measured by reversed-phase HPLC. Results With the increasing of the concentration of Hezi water solution, the content of total tannins increased, pH decreased at first, then kept stable, and the total six alkaloids increased slowly followed by irregularity. When the content ratio of Caowu to Hezi was 2∶1, consistent with Standard of Inner Mongolia Mongolian Medicines, the total alkaloids content was least. Conclusion Concentration of Hezi water solution and storage time changed the content of alkaloids in Zhicaowu. The content ratio of Hezi to Caowu recorded in Standard of Inner Mongolia Mongolian Medicines (in 1986) was scientific and valid.

Key words: Aconiti Kusnezoffii Radix prepared with Fructus Chebulae, processing, excipients, alkaloids content, pH, tannin

中图分类号: 

  • R283.1