主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

北京中医药大学学报 ›› 2017, Vol. 40 ›› Issue (1): 65-69.doi: 10.3969/j.issn.1006-2157.2017.01.013

• 临床研究 • 上一篇    下一篇

阳虚体质者粪便的代谢组学研究*

王均衡,李英帅,骆斌#,岳子敬   

  1. 北京中医药大学 北京 100029
  • 收稿日期:2016-09-12 出版日期:2017-01-30 发布日期:2017-01-30
  • 通讯作者: 骆斌,男,硕士,研究员,硕士生导师,主要研究方向:中医体质与相关疮疡研究,E-mail:luoBinmail1206@163.com
  • 作者简介:王均衡,女,在读硕士生
  • 基金资助:
    *国家自然科学基金青年科学基金项目(No.81302874)

NMR-based metabonomics study on faeces of subjects with yang-deficiency constitution*

WANG Junheng, LI Yingshuai, LUO Bin#,YUE Zijing   

  1. Beijing University of Chinese Medicine, Beijing 100029
  • Received:2016-09-12 Online:2017-01-30 Published:2017-01-30

摘要: 目的 研究阳虚质和平和质 (正常对照)人群粪便中内源性代谢物的差异。方法 收集阳虚体质者及平和体质者各30人的粪便样品,采用基于核磁共振(NMR)的代谢组学方法研究中医阳虚体质及平和体质的粪便;采用多变量统计学方法OSC-PLS分析阳虚体质者的特征代谢物。结果 阳虚质组的粪便中丁酸盐、醋酸盐、蛋氨酸、琥珀酸盐、葡萄糖的浓度低于平和质组;丙酸盐、丙氨酸、乳酸的浓度高于平和质组。结论 阳虚质者粪便与平和体质者比较存在能量代谢、氨基酸代谢及糖代谢的差异,这与阳虚质者脏腑功能和肠道菌群结构有关。

关键词: 阳虚体质, 核磁共振, 代谢组学

Abstract: Objective To compare the endogenous metabolites in faece of subjects with yang-deficiency constitution and the subjects with balanced constitution (healthy persons).Methods Faece samples of 30 persons with yang-deficiency constitution and 30 healthy persons were collected and analyzed by using nuclear magnetic resonance(NMR)-based metabonomics method. And the specific metabolites of faeces of yang-deficiency constitution persons were measured using multivariate statistical analysis OSC-PLS. Results The content of butyrate,acetate,methionine,succinate,glucose in yang-deficiency constitution persons were lower than those in healthy persons, while the content of propionate, alanine, lactate were higher. Conclusion There were differences in metabolism of energy, amino acid and glucose between persons with yang-deficiency constitution and persons with balanced constitution, which should be related to the viscera function and the composition of gut microbiota.

Key words: yang-deficiency constitution, nuclear magnetic resonance (NMR), metabonomics

中图分类号: 

  • R223