主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

北京中医药大学学报 ›› 2017, Vol. 40 ›› Issue (2): 148-154.doi: 10.3969/j.issn.1006-2157.2017.02.012

• 中医药实验研究 • 上一篇    下一篇

阿萨伊对虚热证大鼠环核苷酸、神经及免疫水平的影响*

王子晨1, 王林元2, 朱映黎1, 唐潇然1, 王莎1, 瞿研2, 张建军1#   

  1. 1 北京中医药大学基础医学院 北京 100029;
    2 北京中医药大学中药学院
  • 收稿日期:2016-09-20 出版日期:2017-02-28 发布日期:2017-02-28
  • 通讯作者: 张建军,女,博士,研究员,博士生导师,研究方向: 基于中药基本理论的药效机理及物质基础研究, E-mail: zjj59@163.com
  • 作者简介:王子晨,男,在读硕士生
  • 基金资助:
    北京中医药大学中青年教师资助项目(No.2016-JYB-JSPY-013),北京中医药大学在读研究生资助项目(No.2015-JYB-XS002)

Effect of Aaí on cyclic nucleotides, neurotransmitters and immune level of rats with asthenia pyrosyndrome*

WANG Zichen 1, WANG Linyuan 2, ZHU Yingli 1, TANG Xiaoran1, WANG Sha1, QU Yan2, ZHANG Jianjun 1#   

  1. 1 School of Preclinical Medicine, Beijing University of Chinese Medicine, Beijing 100029;
    2 School of Chinese Materia Medica, Beijing University of Chinese Medicine
  • Received:2016-09-20 Online:2017-02-28 Published:2017-02-28

摘要: 目的 研究阿萨伊对虚热证大鼠环核苷酸、神经及免疫水平的影响。方法 SD大鼠分为空白组,虚热模型组,黄柏组(5 g/kg),葛根芩连汤组(9.6 g/kg),阿萨伊高、低剂量组(3.2、1.6 g/kg);采用每日肌肉注射地塞米松磷酸钠注射液1次,连续21 d的方法制备虚热模型;放免法检测环磷酸腺苷(cAMP)、环磷酸鸟苷(cGMP)含量变化;酶免法检测多巴胺(DA)、多巴胺β羟化酶(D-β-H)、去甲肾上腺素(NE)含量变化;比色法检测免疫球蛋白G(IgG)、免疫球蛋白M(IgM)、补体C3(C3)、补体C4(C4)含量变化,分析黄柏、葛根芩连汤以及阿萨伊对虚热模型作用趋势变化。结果 阿萨伊高剂量组可改善虚热症状,并可降低血清中cAMP、cGMP、cAMP/cGMP的含量(P<0.05);降低血清中DA、D-β-H、NE的含量(P<0.05);升高血清中IgG、IgM、C3的含量(P<0.05,P<0.001,P<0.05)。结论 阿萨伊对环核苷酸、神经及免疫相应指标的调整与黄柏、葛根芩连汤一致,三者同类比较验证了阿萨伊的寒凉药性。

关键词: 阿萨伊, 虚热证, 中药药性, 环核苷酸, 神经递质, 免疫物质, 补体, 大鼠

Abstract: Objective To study the effect of frozen fruit pulp of Aaí (Euterpe Oleraceae Mart.) on cyclic nucleotides, neurotransmitters and immune level of rats with asthenia pyrosyndrome. Methods SD rats were randomly divided into blank group, model group, Huangbo (Cortex Phellodendri Chinensis) group, Gegen Qinlian Decoction (a Chinese herbal medicine compound) group, Aaí high-dose group and Aaí low-dose group. The rat model of asthenia pyrosyndrome was induced by intramuscular injection of dexamethasone sodium phosphate at dose of 0.35 mg/kg once daily for consecutive 21 days. The level of cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP) were detected by radioimmunoassay. The levels of dopamine (DA), dopamine beta-hydroxylase (D-β-H) and norepinephrine (NE) were detected by enzyme immunoassay. The levels of IgG, IgM, C3 and C4 were detected by using colorimeter. The results of the groups were compared to analyze the effect of Huangbo, Gegen Qinlian Decoction and Aaí on rats with asthenia pyrosyndrome. Results High dose Aaí could relieve the symptoms due to asthenia pyrosyndrome, and decreased the serum level of cAMP, cGMP, cAMP/cGMP, DA, D-β-H and NE (P<0.05), while increased the serum content of IgG, IgM and C3 (P<0.05,P<0.001 or P<0.05). Conclusion The effect of Aaí on cyclic nucleotides, neurotransmitters and immune level was similar with that of Huangbo or Gegen Qinlian Decotion, which proved the cold property of Aaí.

Key words: Aaí, asthenia pyrosyndrome, property of Chinese herbal medicine, cyclic nucleotides, neurotransmitter, immunities, complement, rats

中图分类号: 

  • R285.5