主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

北京中医药大学学报 ›› 2017, Vol. 40 ›› Issue (2): 166-171.doi: 10.3969/j.issn.1006-2157.2017.02.015

• 中药制剂 • 上一篇    下一篇

正交设计法优选红芪最佳蜜炙烘制工艺*

李越峰1,2, 牛江涛1,2, 曹瑞1,2, 司昕蕾1,2, 边甜甜1,2, 严兴科1,2#   

  1. 1 甘肃中医药大学 甘肃 730000;
    2 甘肃省中药质量与标准研究重点实验室
  • 收稿日期:2016-10-11 出版日期:2017-02-28 发布日期:2017-02-28
  • 通讯作者: 严兴科,男,博士,教授,博士生导师,研究方向:中药及复方有效成分及质量研究,E-mail: lyfyxk@126.co
  • 作者简介:李越峰,女,博士,教授,硕士生导师
  • 基金资助:
    国家自然科学基金资助项目 (No.81460611),国家教育部科学技术研究重点基金资助项目(No.212186),甘肃省自然科学基金资助项目(No.2010-1010RJZA212,No.2014145RJZA076),甘肃省财政厅高校基本科研业务费专项基金资金项目(No.2013-2),兰州市科技局资助项目(No.2014-1-188),甘肃省中医药管理局资助项目(No.GZK-2015-57)

Optimization of processing technology of honey barked Hedysari Radix using orthogonal design method*

LI Yuefeng1,2, NIU Jiangtao1,2, CAO Rui1,2, SI Xinlei1,2, BIAN Tiantian1,2, YAN Xing Ke1,2#   

  1. 1 Gansu University of Chinese Medicine,Gansu 730000;
    2 Gansu Provincial Key Laboratory of Standard and Quality of Chinese Medicine Research
  • Received:2016-10-11 Online:2017-02-28 Published:2017-02-28

摘要: 目的 优选红芪蜜炙最佳烘制炮制工艺,为红芪炮制品质量控制及标准化炮制提供实验依据。方法 采用L9(34)正交设计法,以毛蕊异黄酮和芒柄花素含量的平均值为综合评价指标,优选红芪蜜炙烘制炮制工艺。采用高效液相色谱法(HPLC),梯度洗脱,测定红芪中的毛蕊异黄酮和芒柄花素含量。 结果 红芪蜜炙最佳烘制炮制工艺为:烘制温度70 ℃,烘制时间2.5 h,厚度3 cm。结论 红芪烘制炮制品能够达到2015版《中国药典》要求,且方法简单可行,重现性好,可为进一步研究红芪最佳炮制工艺提供科学依据,为红芪炮制工业生产规范化提供科学依据。

关键词: 红芪, 蜜炙烘制, 正交设计

Abstract: ObjectiveTo optimize the processing technology of honey baked Hedysari Radix, so as to provide the experimental basis for the quality control of Hedysari Radix processed products and standardization of the processing. Methods L9 (34) orthogonal design method was used, and the mean content of calycosin and formononetin which were detected by HPLC with gradient elution were taken as the comprehensive evaluation indexes, the processing technology was optimized. Results The optimum processing technology of honey baked Hedysari Radix was thickness of 3 cm at the temperature of 70 ℃ for 2.5 h.Conclusion Hedysari Radix baking processed products produced by the optimum technology met with the requirement of Chinese Pharmacopoeia (2015 Edition), which was simple, feasible, and repeatable. The method would provide evidence for further research on optimum processing technology and for standardiztion of industrial production.

Key words: Hedysari Radix, honey baked, orthogonal design method

中图分类号: 

  • R283.3