主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

北京中医药大学学报 ›› 2017, Vol. 40 ›› Issue (5): 420-427.doi: 10.3969/j.issn.1006-2157.2017.05.011

• 中药化学 • 上一篇    下一篇

基于金花葵核苷类成分的质量表征关联分析研究*

孔静, 贺晶1, 谭立1, 申立峰2, 李倩2, 张元元1, 吕珊1, 武秋红1, 陈梦杰1, 刘璐1, 李文霞1, 路东波1, 石任兵1,3, 姜艳艳1,3#   

  1. 1 北京中医药大学中药学院 国家中医药管理局中药经典名方有效物质发现重点实验室 北京 100102;
    2 首都医科大学中医药学院;
    3 北京市教委中药质量控制技术工程中心
  • 收稿日期:2017-01-27 出版日期:2017-05-30 发布日期:2017-05-30
  • 通讯作者: 姜艳艳,女,博士,副教授,硕士生导师,研究方向:中药(复方)有效物质基础研究及质量控制方法研究,E-mail:jyyjm1129@163.com
  • 作者简介:孔静,女,在读硕士生
  • 基金资助:
    *十二五国家科技支撑计划项目(No.2012BAI29B06),北京市教委共建科研基地建设项目,北京中医药大学重点学科开放课题(No.2013-2DXKKF-23)

Relevant analysis on quality representation based on nucleosides in Aurea helianthus*

KONG Jing1, HE Jing1, TAN Li1, SHEN Lifeng2, LI Qian2, ZHANG Yuanyuan1, LV Shan1, WU Qiuhong1, CHEN Mengjie1, LIU Lu1, LI Wenxia1, LU Dongbo1, SHI Renbing1,3, JIANG Yanyan1,3#   

  1. 1 Key Unit of Exploring Effective Substances of Classical and Famous Formulas of State Administration of Traditional Chinese Medicine, School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China;
    2 School of Chinese Medicine, Capital University of Medical Sciences, Beijing 100069, China;
    3 Quality Control Technology and Engineering Center of Chinese Medicine, Beijing Municipal Commission of Education, Beijing 100102, China
  • Received:2017-01-27 Online:2017-05-30 Published:2017-05-30
  • Supported by:
    Project sporsored by National Sci-Tech Support Plan of the 12th Five-Year Plan (No.2012BAI29B06), Joint project of Research Base of Beijing Municipal Education Commission

摘要: 目的 建立同时测定金花葵药材中7种核苷类成分(尿嘧啶、胞苷、尿苷、腺嘌呤、鸟苷、腺苷、2’-脱氧腺苷)含量的高效液相色谱法(HPLC),并对金花葵药材进行质量关联分析与评价。方法 采用HPLC-光电二级管阵列(PDA)法测定金花葵药材中7种核苷类成分,基于7个指标性成分含量及指标性成分加和含量对11批金花葵进行质量表征,并对表征结果进行关联性分析。结果 7种核苷类成分线性关系良好,精密度、稳定性、重复性、加样回收率均符合方法学测定要求,并应用所建立的方法测定了11批金花葵药材中7种核苷类成分的含量;以批号1为基准药材,批号2、6、9、10、4各指标成分总体含量高于基准药材,批号6、9、8、4、3、11与基准药材关联性最高,综合评价得出批号1、2、6、9、4优良度居前。结论 所建立的金花葵药材中核苷类成分的含量测定方法及质量表征关联分析模式能分析金花葵质量整体关联性与应用有效性,为金花葵药材的质量控制及评价提供了可靠依据。

关键词: 金花葵, 核苷, 高效液相色谱法, 含量测定, 关联分析, 质量评价

Abstract: Objective To establish a method of high performance liquid chromatography (HPLC) for simultaneously determining of 7 nucleoside (uracil, cytidine, uridine, adenine, guanosine, adenosine and 2'-deoxyadenosine) in Aurea helianthus, and conduct the relevant analysis and review on quality of Aurea helianthus. Methods HPLC-PDA was applied for determining 7 nucleosides in Aurea helianthus. The quality representation of 11 batches of Aurea helianthus was carried out and representation results were given relevant analysis based on the content of index components and index component adducts from 7 nucleosides. Results The linear relations of 7 nucleosides were good, and precision, stability, repeatability and sample recoveries were all accorded with the requirements of methodology determination. The content of 7 nucleosides in 11 batches of Aurea helianthus was determined by using the established method. Taking batch lot 1 of Aurea helianthus as criterion material, the content of index components was higher in batch lot 2, 6, 9, 10 and 4, and relevancy was the highest among criterion Aurea helianthus and batch lot 6, 9, 8, 4, 3 and 11. The comprehensive review showed that excellent degrees of batch lot 1, 2, 6, 9 and 4 were in the lead. Conclusion The established method for determining content of nucleosides in Aurea helianthus and correlation analysis mode of quality representation can analyze overall quality relevancy and application validity, which provides the reliable evidence for quality control and review of Aurea helianthus.

Key words: Aurea helianthus, nucleosides, high performance liquid chromatography, content determination, relevant analysis, quality review

中图分类号: 

  • R284.2