主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

北京中医药大学学报 ›› 2017, Vol. 40 ›› Issue (7): 558-563.doi: 10.3969/j.issn.1006-2157.2017.07.006

• 科技之窗 • 上一篇    下一篇

半夏泻心汤和鸟嘌呤核苷对APPswe/PS1dE9双转基因小鼠学习记忆的影响*

盛宁1, 何瑛琨1,2, 冯慧利1, 董云芳1, 樊惠1,3, 党惠子1,4, 任映1, 杨金铎, 王蓬文1#   

  1. 1 北京中医药大学东直门医院中医内科学教育部和北京市重点实验室 北京 100700;
    2 河北省人民医院;
    3 北京朝阳区中西医结合急诊抢救中心;
    4 北京市回民医院
  • 收稿日期:2017-01-15 出版日期:2017-07-10 发布日期:2017-07-10
  • 通讯作者: 王蓬文,女,博士,教授,博士生导师,主要研究方向:神经变性病的发病机制及中医药防治策略,E-mail:pw_wang@163.com
  • 作者简介:盛宁,男,在读硕士生
  • 基金资助:
    国家自然科学基金面上资助项目(No. 81573927),北京市教育委员会科学研究与研究生培养共建资助项目(No. 2016)

Influences of Banxia Xiexin Tang and guanosine on learning and memory in APPswe/PS1dE9 double transgenic mice*

SHENG Ning1, HE Yingkun1,2, FENG Huili1, DONG Yunfang1, FAN Hui1,3, DANG Huizi1,4, REN Ying1, YANG Jinduo, WANG Pengwen1#   

  1. 1 Education Ministry and Beijing Key Laboratory of Chinese Internal Medicine, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China;
    2 Hebei General Hospital, Hebei 050051, China;
    3 Beijing Chaoyang Integrative Medicine-Emergency Medical Center, Beijing 100022, China;
    4 Beijing Huiming Hospital, Beijing 100053, China
  • Received:2017-01-15 Online:2017-07-10 Published:2017-07-10
  • Supported by:
    National Natural Science Foundation of China (No. 81573927), Scientific Research and Graduate Training Joint Project of Beijing Municipal Education Commission (No. 2016)

摘要: 目的 采用跳台和Morris水迷宫检测半夏泻心汤和鸟嘌呤核苷对6月龄APPswe/PS1dE9双转基因小鼠学习记忆的影响。方法 将APPswe/PS1dE9双转基因小鼠随机分为模型组5%羧甲基纤维素钠(CMC)灌胃、西药对照组盐酸多奈哌齐0.92 mg/(kg·d)灌胃、半夏泻心汤组650 mg/(kg·d)灌胃、中药对照组姜黄素200 mg/(kg·d)灌胃、鸟嘌呤核苷组0.5%CMC灌胃,除鸟嘌呤核苷组腹腔注射鸟嘌呤核苷16 mg/(kg·d),其他各组腹腔注射等体积的生理盐水,每组15只。正常组0.5%CMC灌胃和等体积的生理盐水腹腔注射为同窝野生型C57BL/6J小鼠。于3月龄时开始对各组小鼠分别实施对应的干预,给药3个月后,应用跳台和Morris水迷宫对小鼠进行行为学检测。结果 跳台实验结果显示,模型组小鼠比正常组潜伏期时间明显缩短(P<0.01);与模型组比,各干预组小鼠潜伏期时间均显著增加(P<0.01)。Morris水迷宫结果显示,随着天数的增加,各组小鼠逃避潜伏期的时间缩短,游泳距离也呈递减趋势,中间有小幅波动。Morris水迷宫结果显示,空间探索实验即撤台实验中,模型组小鼠目标象限停留时间显著少于正常组(P<0.01),西药对照组、中药对照组以及半夏泻心汤组小鼠的目标象限停留时间均比模型组延长(P<0.05)。结论 半夏泻心汤对APPswe/PS1dE9双转基因小鼠的早期学习记忆障碍具有改善作用,鸟嘌呤核苷可提高小鼠的记忆保持能力。

关键词: 鸟嘌呤核苷, 半夏泻心汤, 阿尔茨海默病, 行为学, 小鼠

Abstract: Objective To detect the influences of Banxia Xiexin Tang (Pinellia Heart-Draining Decoction) and guanosine on learning and memory in APPswe/PS1dE9 double transgenic mice (6 months old) by applying step-down test and Morris water maze (MWM). Methods The mice were randomly divided into model group (given 0.5% CMC intragastrically), Western drug control group [WD group, given donepezil in 0.92 mg/(kg·d) intragastrically], Banxia Xiexin Tang group [BXT group, given Banxia Xiexin Tang in 650 mg/(kg·d) intragastrically], Chinese medicinal control group [CM group, given curcumin in 200 mg/(kg·d)] and guanosine group (given 0.5%CMC intragastrically, each n=15). The guanosine group was given intraperitoneal injection of guanosine [16 mg/(kg·d)], and other groups were given intraperitoneal injection of isopyknic normal saline (NS). The brood wide type C57BL/6J mice (n=15) were set into normal group and given 0.5%CMC intragastrically and isopyknic NS. All groups were given corresponding interventions when mice were 3 months old. The ethological detection was conducted by using step-down test and MWM in al groups after 3 m. Results The results of step-down test showed that latency was significantly shorter in model group than that in normal group (P<0.01), and was significantly longer in all medicinal intervention groups than that in model group (P<0.01). The results of MWM showed that escape latency was shortened, swimming distance showed decline trend with slight fluctuation in all groups along with days increasing. The results of MWM showed that in spatial probe test, the time of targeting quadrant was significantly shorter in model group than that in normal group (P<0.01), and was longer in WD group, CM group and BXT group than that in model group (P<0.05). Conclusion Banxia Xiexin Tang has an ameliorative effect on early learning and memory disorder, and guanosine can improve the ability of memory retaining in APPswe/PS1dE9 double transgenic mice.

Key words: guanosine, Banxia Xiexin Tang (Pinellia Heart-Draining Decoction), Alzheimer’s disease, ethology, mice

中图分类号: 

  • R285.5