主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

北京中医药大学学报 ›› 2017, Vol. 40 ›› Issue (8): 661-668.doi: 10.3969/j.issn.1006-2157.2017.08.008

• 科技之窗 • 上一篇    下一篇

当归红芪超滤膜提取物对12C6+重离子束辐射致H22荷瘤小鼠免疫功能损伤的影响

刘凯1,2, 柴辉2, 孙少伯2, 李应东1,2#, 王伟3   

  1. 1 兰州大学基础医学院 甘肃730000;
    2 甘肃中医药大学;
    3 北京中医药大学
  • 收稿日期:2017-04-03 出版日期:2017-08-10 发布日期:2017-08-10
  • 通讯作者: 李应东,男,博士,教授,博士生导师,研究方向:中西医结合防治肿瘤,E-mail:lydj412@163.com
  • 作者简介:刘凯,男,在读博士生,副教授
  • 基金资助:
    国家自然科学基金项目(No.81160478)

Effects of ultra-filtration extract from Danggui and Hongqi on immune function injury induced by 12C6+heavy ion beam radiationin H22 tumor-bearing mice*

LIU Kai1,2,CHAI Hui 2, SUN Shaobo2, LI Yingdong1,2#, Wang Wei3   

  1. 1 School of Preclinical Medicine, Lanzhou University, Gansu 730000, China;
    2 Gansu University of Chinese Medicine, Gansu 730000, China;
    3 Beijing University of Chinese Medicine, Beijing 100029, China
  • Received:2017-04-03 Online:2017-08-10 Published:2017-08-10
  • Supported by:
    National National Science Foundation of China(No.81160478)

摘要: 目的 观察当归红芪超滤膜提取物抗12C6+重离子束辐射致H22荷瘤小鼠免疫功能损伤的作用。方法 H22细胞皮下注射法构建H22荷瘤小鼠模型,以检测血液白细胞数目为评价标准确定12C6+重离子束辐射致H22荷瘤小鼠免疫损伤最佳辐射剂量及当归红芪超滤膜提取物药物干预最佳剂量。实验分正常对照组、肿瘤模型组、单纯药物组(给予当归红芪超滤膜提取物6.72 g/kg)、单纯放射组(照射剂量为4Gy)、药物(给予当归红芪超滤膜提取物6.72 g/kg)+放射(照射剂量为4Gy)组。测定各组脾脏指数、胸腺指数;流式细胞仪技术分析各组小鼠血液T淋巴细胞亚群变化;ELISA法检测各组小鼠血清补体C3、干扰素-γ(IFN-γ)、白细胞介素-4(IL-4)含量;光镜下观察脾脏病理形态学改变。结果 与肿瘤模型组比较,单纯放射组血液白细胞数目、脾脏指数、胸腺指数、血液CD3+ 、CD4+、CD8+ T淋巴细胞数量、血清补体C3、IFN-γ、IL-4 含量明显下降(P<0.05),CD4+/CD8+比值、IFN-γ/IL-4比值明显下降(P<0.05)。病理形态学显示:肿瘤模型组小鼠脾脏形态结构如常,多数脾窦扩张淤血,脾小体清晰,可见动脉周围淋巴鞘,巨核细胞多见,与正常对照组小鼠脾脏形态无明显差异;单纯放射组脾脏脾小体及红髓脾索萎缩,淋巴细胞明显减少,巨核细胞数量减少,纤维组织增生。与单纯放射组比较,药物+放射组血液白细胞数目、脾脏指数、胸腺指数、血液CD3+ 、CD4+、CD8+ T淋巴细胞数量、血清补体C3、IFN-γ含量明显上升(P<0.05),CD4+/CD8+比值、IFN-γ/ IL-4比值有明显上升(P<0.05),病理形态学损伤改变明显减轻。结论 当归红芪超滤膜提取物有减轻12C6+重离子束辐射引起H22的荷瘤小鼠免疫功能损伤的作用。

关键词: 当归, 红芪, 12C6+重离子束, H22荷瘤小鼠, 免疫功能

Abstract: Objective To observe the effects of ultra-filtration extract from Danggui (Radix Angelicae Sinensis, Chinese Angelica) and Hongqi (Radix Hedysair, Sweetvetch Root) on immune dysfunction caused by 12C6+ heavy ion beam radiation in H22 tumor-bearing mice. Methods Tumor-bearing mice model was built by subcutaneously injecting H22 cells. Blood leukocytes count was used to determine the optimal radiation dose of immune injury induced by heavy ion beam irradiation and the optimal dose of ultra-filtration extract from Danggui and Hongqi. The mice were divided into normal control group, tumor model group, drug intervention group (6.72 g/kg), radiation group (4Gy), and drug (6.72 g/kg) plus radiation (4Gy) group. Spleen index and thymus index were measured in each group; Changes of T lymphocyte in each group was detected by using flow cytometry; Levels of serum C3, IFN-γ and IL-4 were tested with ELLSA; Spleen pathological and morphological changes were observed under optical microscope.Results Compared with the model group, blood leukocyte count, spleen index, thymus index, levels of CD3+, CD4+ and CD8+ T lymphocytes, serum C3, IFN-γ and IL-4 in radiation group decreased significantly (P<0.05), and ratio of CD4+/CD8+ and IFN-γ/IL-4 in radiation group decreased significantly (P<0.05).Pathological morphology indicated that there was no significant difference between the model group and normal control group. Spleen structure was normal; most of the splenic sinus dilated and developed blood stasis.Splenic corpuscle was clear. The lymphatic sheath around the artery was present, and macronuclear cells could be observed in the model group.Whereas in the radiation group, the spleen body and red pulp spleen cord shrank; the number of lymphocyte and megakaryocytes reduced, and fibrous tissue proliferated. Compared with the radiation group, blood leukocytes count, spleen index, thymus index, the levels of CD3+, CD4+ and CD8+ T lymphocytes, serum C3, IFN-γ and IL-4 in drug plus radiation group increased significantly (P<0.05), and the ratio of CD4+/CD8+ and IFN-γ/IL-4 in drug plus radiation group increased markedly (P<0.05). Compared with the radiation group, pathological changes of spleen in the drug plus radiation group alleviated significantly. Conclusions The ultra-filtration extract from Danggui and Hongqi can could alleviate the immune function injury caused by 12C6+ heavy ion beam radiation in H22 tumor-bearing mice.

Key words: Danggui, Hongqi, 12C6+ heavy ion beam, H22 tumor-bearing mice, immune function

中图分类号: 

  • R285.5