主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

北京中医药大学学报 ›› 2017, Vol. 40 ›› Issue (8): 684-692.doi: 10.3969/j.issn.1006-2157.2017.08.011

• 中药化学 • 上一篇    下一篇

基于酚类特征图谱的山楂质量表征关联分析研究

赵博1, 田恒康2, 孙道涵1, 申立峰3, 孔静1, 路东波1, 李文霞1, 武秋红1, 刘璐1, 李倩3张元元1, 吕珊1, 陈梦杰1, 石任兵1,4#   

  1. 1 北京中医药大学中药学院国家中医药管理局中药经典名方有效物质发现重点实验室 北京 100102;
    2 国家食品药品监督管理局药品审评中心;
    3 首都医科大学中医药学院;
    4 北京市教委中药质量控制技术工程中心
  • 收稿日期:2017-05-08 出版日期:2017-08-10 发布日期:2017-08-10
  • 通讯作者: 石任兵,男,博士,教授,博士生导师,研究方向:中药(复方)有效物质基础研究与药物创新,E-mail: shirb@126.com
  • 作者简介:赵博,女,在读硕士生
  • 基金资助:
    十二五国家科技支撑计划项目(No.2012BAI29B06),北京中医药大学重点学科开放课题(No.2013-2DXKKF-23)

Quality representation and correlation analysis of Shanzha based on phenolic characteristic spectrum of medical system*

ZHAO Bo1, TIAN Hengkang2, SUN Daohan1, SHEN Lifeng3, KONG Jing1, LU Dongbo1, LI Wenxia1, WU Qiuhog1, LIU Lu1, LI Qian3, ZHANG Yuanyuan1, LYU Shan1,CHEN Mengjie1, SHI Renbing1,4#   

  1. 1 Key Unit of Exploring Effective Substances of Classical and Famous Formulas of State Administration of Traditional Chinese Medicine, School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China;
    2 Center of Drug Evaluation, Beijing 100038, China;
    3 School of Chinese Medicine, Capital Medicinal University Beijing 100069, China;
    4 Quality Control Technology and Engineering Center of Chinese Medicine, Beijing Municipal Commission of Education, Beijing 100069, China
  • Received:2017-05-08 Online:2017-08-10 Published:2017-08-10
  • Supported by:
    National Sci-Tech Support Plan of the 12th Five-Year Plan (No.2012BAI29B06)

摘要: 目的 建立基于药物体系的山楂特征图谱质量表征关联分析方法与评价模式,有效、精准地评价山楂饮片质量。方法 运用所建立的 HPLC-PDA 法表征山楂药物体系特征图谱质量,基于特征图谱中特征峰的数量及所属化学类型对11批山楂饮片进行质的表征,基于特征图谱中特征指标性成分金丝桃苷、表儿茶素、原儿茶酸和绿原酸的含量及黄酮类(以金丝桃苷、表儿茶素表征)、酚酸类(以原儿茶酸和绿原酸表征)的含量对11批山楂饮片进行量的表征,并基于基准饮片将11批山楂饮片质与量的表征结果分别进行关联性分析。结果 以批号1的样品为基准饮片,山楂饮片特征图谱中共含有特征峰13个,其中黄酮类成分特征峰7个,酚酸类特征峰6个,11批饮片色谱图中均含有此13个特征峰。批号5、10、8、2、6样品特征指标性成分含量总体较高,批号6、4、3、7、8、2样品与基准饮片关联性最高,综合评价得出批号5、10、8、2、7、3样品良度居前。结论 对11批山楂饮片进行特征图谱质量表征,所建立的 HPLC 法简便、准确。所评价建立的基于药物体系的山楂特征图谱质量表征关联分析模式,关注了山楂饮片质量整体关联性与应用有效性,能有效、精准地评价山楂饮片质量。

关键词: 山楂, 特征图谱, 质量评价, 关联分析

Abstract: Objective To establish quality representation and correlation analysis method based on the spectrum of medicinal system of Shanzha(Fructus Crataegi, Chinese hawthorn fruit),so as to evaluate quality of Shanzha decocting pieces effectively and accurately. Methods HPLC-PDA method was applied to characterize quality of spectrum of Shanzha medicinal system; quality of 11 batches of Shanzha decocting pieces were characterized based on the number and chemical type of peaks on characteristic spectrum.The content of characteristic index components, protocatechuic acid, epicatechin,chlorogenicacid, phenolic acids (represented by protocatechuic acid and chlorogenic acid) and flavonoids (represented by hyperoside and epicatechin) were characterized by indexes in characteristic spectrum. Correlation analysis of quality and quantity of Shanzha was then performed with reference to the standard reference pieces.Results As the reference substance, characteristic spectrum of batch 1 contains 13 characteristic peaks, 7 peaks of flavonoids and 6 peaks of phenolic acids.All characteristic peaks appeared on the chromatograms of 11 batches of Shanzha.Batch 5,10, 8, 2,6 had more effective index ingredients. After comprehensive evaluation,there was a high correlation between batch 6, 4, 3, 7, 8, 2 and standard reference pieces. The quality of batch 5, 10, 8, 2, 7, and 3 ranked on the top among all samples.Conclusion HPLC method to characterize quality and quantity of characteristic spectrum of 11 batches of Shanzha is simple and accurate. The evaluation mode of quantity and quality and their correlation based on Shanzha medical system, including systemic correlation and application validity, can evaluate the quality of hawthorn pieces effectively and accurately.

Key words: Shanzha, characteristic spectrum, quality evaluation, correlation analysis ,  ,  

中图分类号: 

  • R284.1