主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

北京中医药大学学报 ›› 2017, Vol. 40 ›› Issue (9): 729-735.doi: 10.3969/j.issn.1006-2157.2017.09.005

• 博士之光 • 上一篇    下一篇

脾虚1号方对功能性消化不良脾虚证大鼠胃体组织MLC蛋白与基因表达的影响*

吕林1,王凤云1,唐旭东1#,尹晓岚2,石啸双2,段园志2   

  1. 1 中国中医科学院西苑医院消化科 北京市中医脾胃病研究所 北京 100091;
    2 中国中医科学院研究生院
  • 收稿日期:2017-04-06 出版日期:2017-09-10 发布日期:2017-09-10
  • 通讯作者: 唐旭东,男,博士,主任医师,博士生导师,研究方向:中医药治疗消化系统疾病,E-mail:txdly@sina.com.cn
  • 作者简介:吕林,男,博士,主治医师
  • 基金资助:
    *国家重点基础研究发展计划(973计划)资助项目 (No.2013CB531703),国家自然科学基金资助项目(No.81503567),中国博士后科学基金资助项目(No.2015M1227,2016T90195)

Influence of Pixu Fang 1 (Spleen-deficiency Formula 1) on protein and gene expressions of myosin light chain in rats with functional dyspepsia-spleen deficiency pattern*

LYU Lin1, WANG Fengyun1, TANG Xudong1#, YIN Xiaolan2, SHI Xiaoshuang2, DUAN Yuanzhi2   

  1. 1 Department of Gastroenterology, Beijing Institute of Spleen and Stomach Diseases, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China;
    2 Graduate School, China Academy of Chinese Medical Sciences, Beijing 100700, China
  • Received:2017-04-06 Online:2017-09-10 Published:2017-09-10
  • Supported by:
    National Key Basic Research Development Plan (973 Plan, No.2013CB531703), National Natural Science Foundation of China (No.81503567), China Postdoctoral Science Foundation (No.2015M1227, 2016T90195)

摘要: 目的 探讨功能性消化不良(FD)脾虚证大鼠胃体组织肌球蛋白轻链(MLC)蛋白与mRNA表达及脾虚1号方的干预作用。方法 60只7 d龄雄性SD大鼠随机分为正常对照组(n=10)、FD脾虚证模型组(n=50)。正常组给予2%蔗糖溶液灌胃,FD脾虚证模型组给予0.1%蔗糖碘乙酰胺溶液灌胃,0.2 mL/(只·d),连续6 d。FD脾虚证模型组正常饲料喂养至6周龄后叠加改良小平台站立,连续14 d。造模结束后随机分为模型组、多潘立酮组、脾虚1号方低、中、高剂量组,各10只,连同正常组,分别给予蒸馏水10 mL/(kg·d)、多潘立酮3.125 mg/(kg·d)、脾虚1号方1.275、2.550、5.100 g/(kg·d),灌胃14 d。采用免疫组化、蛋白质印迹(Western blot)和RT-PCR方法检测胃体组织MLC蛋白及mRNA表达量。结果 与正常组相比,免疫组化检测模型组大鼠胃体肌层MLC蛋白定位表达降低;Western blot检测模型组胃体组织MLC蛋白表达量降低;RT-PCR检测模型组大鼠胃体组织MLC mRNA表达量降低,差异均有统计学意义(P<0.01)。与模型组相比,免疫组化检测脾虚1号方低、中、高剂量组大鼠胃体肌层MLC蛋白定位表达增加,差异均有统计学意义(P<0.05,P<0.01);Western blot检测脾虚1号方中、高剂量组大鼠胃体MLC蛋白表达量增加,差异均有统计学意义(P<0.01);RT-PCR检测脾虚1号方高剂量组MLC mRNA表达量升高,差异有统计学意义(P<0.01)。结论 FD脾虚证模型大鼠存在胃体组织MLC蛋白及基因表达的降低,而脾虚1号方能够上调MLC蛋白及基因的表达。

关键词: 脾虚1号方, 功能性消化不良, 脾虚证, 胃动力, 肌球蛋白轻链, 大鼠

Abstract: Objective To investigate the intervention of Pixu Fang 1 (Spleen-deficiency Formula 1) to the protein and mRNA expressions of myosin light chain (MLC) in gastric tissue in rats with functional dyspepsia (FD)-spleen deficiency pattern (SDP). Methods SD rats (n=60, aged 7 d) were randomly divided into normal group (n=10) and FD-SDP model group (n=50). The normal group was orally given 2% sugar solution and FD-SDP model group was given 0.1% iodoacetamide sugar solution in a dose of 0.2 mL each rat a day for 6 d. The FD-SDP model group was fed with normal forage until rats aged 6 weeks and additionally given modified multiple platform method (MMPM) continuously for 14 d. After modeling, FD-SDP model group was divided randomly into model group (distilled water, 10 mL/kg·d), domperidone group (3.125 mg/kg·d), low-dose Pixu Fang 1 group (low-dose group, 1.275 g/kg·d), mid-dose Pixu Fang 1 group (mid-dose group, 2.550 g/kg·d) and high-dose Pixu Fang 1 group (high-dose group, 5.100 g/kg·d, each n=10), and all groups were given orally corresponding medicinal for 14 d. The protein and mRNA expressions of MLC in gastric tissue were detected by using immunohistochemistry technique, Western blotting and reverse transcription-polymerase chain reaction (RT-PCR). Results Compared with normal group, MLC protein expression decreased in model group detected by immunohistochemistry technique and Western blotting, and MLC mRNA expression decreased in model group detected by RT-PCR (P<0.01). Compared with model group, MLC protein expression increased in low-dose group, mid-dose group and high-dose group detected by immunohistochemistry technique (P<0.05, P<0.01). MLC protein expression increased in mid-dose group and high-dose group detected by Western blotting (P<0.01). MLC mRNA expression increased in high-dose group detected RT-PCR (P<0.01). Conclusion There are decreases of protein and mRNA expressions of MLC in FD-SDP rats, and Pixu Fang 1 can up-regulate these expressions.

Key words: Pixu Fang 1 (Spleen-deficiency Formula 1), functional dyspepsia, spleen deficiency pattern, gastric dynamics, myosin light chain, rats

中图分类号: 

  • R285.5