主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

北京中医药大学学报 ›› 2017, Vol. 40 ›› Issue (9): 743-749.doi: 10.3969/j.issn.1006-2157.2017.09.007

• 科技之窗 • 上一篇    下一篇

杞红方对寒凝血瘀衰老大鼠心脏保护作用的实验研究*

张睿1,冯玄烨1,王湘1,刘琳琳1,戴宁1,冯丽莉1,孙燕1,赵欣2,蔡大勇2,王玥琦1#   

  1. 1 北京中医药大学中医学院 北京 100029;
    2 中国医学科学院药用植物研究所
  • 收稿日期:2017-03-27 出版日期:2017-09-10 发布日期:2017-09-10
  • 通讯作者: 王玥琦,女,博士,教授,博士生导师,研究方向:中药延缓衰老的作用及其机理研究,E-mail:yueqiwng@gmail.com
  • 作者简介:张睿,男,在读硕士生
  • 基金资助:
    *教育部重点研究基金资助项目(No.108019)

Heart protective effect of Qihong Fang (Wolfberry and Hawthorn Decoction) in aging rats with cold-congealing and blood-stasis pattern*

ZHANG Rui1, FENG Xuanye1, WANG Xiang1, LIU Linlin1, DAI Ning1, FENG Lili1, SUN Yan1, ZHAO Xin2, CAI Dayong2, WANG Yueqi1#   

  1. 1 School of Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China;
    2 Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China
  • Received:2017-03-27 Online:2017-09-10 Published:2017-09-10
  • Supported by:
    Key Research Foundation from Ministry of Education (No. 108019

摘要: 目的 探究杞红方对寒凝血瘀衰老大鼠心脏的影响。方法 48只SD大鼠随机分为空白对照组,模型组,维生素E组(13.5 mg/kg),杞红方低、中、高剂量(45、90、180 mg/kg)组。除空白对照组外,其余5组运用28 d臭氧致衰复合14 d冰水致寒凝血瘀造模法,制作寒凝血瘀衰老大鼠模型。第29天腹主动脉取血分离血清、血浆,分离心脏,检测血清总超氧化物歧化酶(T-SOD)、铜锌超氧化物歧化酶(Cu-Zn-SOD)、锰超氧化物歧化酶(Mn-SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)的活力,丙二醛(MDA)含量,血浆凝血4项(PT、APTT、TT、FIB)。通过HE染色观察心脏细胞形态,Masson染色观察纤维沉积量,免疫组化检测转化生长因子β1(TGF-β1)表达量以评价寒凝血瘀衰老模型,并观察杞红方对大鼠心脏的保护作用。结果 与空白对照组相比,模型组T-SOD、Cu-Zn-SOD、Mn-SOD、CAT、GSH-Px活力明显降低(P<0.05),MDA含量明显升高(P<0.05),PT、APTT明显降低(P<0.05),FIB含量明显升高(P<0.05),光镜下Masson染色,形态计量心脏胶原纤维沉积明显增加(P<0.05),TGF-β1表达量明显升高(P<0.05)。与模型组相比,杞红方中、高剂量均能明显回升T-SOD、Cu-Zn-SOD、CAT、GSH-Px活力(P<0.05),明显回降MDA含量(P<0.05),明显回升PT、ATPP(P<0.05),明显回降FIB含量(P<0.05),明显回降心脏细胞胶原纤维沉积(P<0.05),明显回降TGF-β1表达量(P<0.05)。结论 杞红方对寒凝血瘀衰老大鼠的心脏具有保护作用。

关键词: 杞红方, 寒凝血瘀, 衰老, 心脏, 转化生长因子-β1, 大鼠

Abstract: Objective To discuss the influence of Qihong Fang (Wolfberry and Hawthorn Decoction) on heart in aged rats with cold-congealing and blood-stasis pattern. Methods SD rats (n=48) were divided randomly into normal control group, model group, vitamin E group (13.5 mg/kg), low-dose Qihong Fang group (45 mg/kg), mid-dose Qihong Fang group (90 mg/kg) and high-dose Qihong Fang group (180 mg/kg). The aged rat model of cold-congealing and blood-stasis pattern was established through 28-d ozone leading to aging and 14-d ice water leading to cold-congealing and blood-stasis pattern in other 5 groups except of control group. On the 29th d, the blood samples were collected from abdominal aorta for isolating serum and plasma, and heart was separated too. The vitalities of serum total superoxide dismutase (T-SOD), Cu-Zn SOD, Mn-SOD, catalase (CAT), glutathione peroxidease (GSH-PX), content of malondialdehyde (MDA), and prothrombin time (PT), activated partial thromboplastin time (APTT), thrombin time (TT) and fibrinogen (FIB) were detected. The morphologic changes of heart cells were observed after HE staining, fiber deposition quantity was observed after Masson staining, expression of transforming growth factor β1 (TGF-β1) was detected by using immunohistochemistry technique for reviewing aging model of cold-congealing and blood-stasis pattern, and protective effect of Qihong Fang on heart was observed in rats. Results Compared with normal control group, the vitalities of T-SOD, Cu-Zn-SOD, Mn-SOD, CAT and GSH-PX decreased significantly (P<0.05), MDA content increased significantly (P<0.05), PT and APTT decreased significantly (P<0.05), and FIB content increased significantly (P<0.05) in model group. After Masson staining, morphometry observation by light microscope showed deposition of heart collagen fibers increased significantly (P<0.05), and expression of TGF-β1 increased significantly (P<0.05). Compared with control group, the vitalities of T-SOD, Cu-Zn-SOD, CAT and GSH-PX increased significantly (P<0.05), MDA content decreased significantly (P<0.05), PT and ATPP increased significantly (P<0.05), FIB content decreased significantly (P<0.05), deposition of heart collagen fibers decreased significantly (P<0.05), and expression of TGF-β1 decreased significantly (P<0.05) in mid-dose and high-dose Qihong Fang groups. Conclusion Qihong Fang has a protective effect on heart in aged rats with cold-congealing and blood-stasis pattern.

Key words: Qihong Fang (Wolfberry and Hawthorn Decoction), cold-congealing and blood-stasis, senility, heart, transforming growth factor -β1, rats

中图分类号: 

  • R285.5