主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

北京中医药大学学报 ›› 2017, Vol. 40 ›› Issue (9): 758-763.doi: 10.3969/j.issn.1006-2157.2017.09.009

• 中药化学 • 上一篇    下一篇

蒙药复方述达格-4的大鼠血清化学成分分析*

信莎莎,杜银飞,董玉#,张天明,彭璐   

  1. 内蒙古医科大学药学院 内蒙古 010110
  • 收稿日期:2017-04-07 出版日期:2017-09-10 发布日期:2017-09-10
  • 通讯作者: 董玉,女,教授,博士,博士生导师,研究方向:中蒙药(复方)有效物质基础研究及药物创新,E-mail:dongyu010@126.com
  • 作者简介:信莎莎,女,在读硕士生
  • 基金资助:
    *国家自然科学基金资助项目(No.81260680),2014年内蒙古自治区高等学校“青年科技英才支持计划”青年科技领军人才项目(No.NJYT-14-A12),2016年内蒙古自治区科技创新引导奖励基金,内蒙古自治区大学生创新创业训练计划项目(No.201610132002)

Chemical compositions of Mongolian medicine compound formula-Shudage-4 in rat serum*

XIN Shasha, DU Yinfei, DONG Yu#, ZHANG Tianming, PENG Lu   

  1. School of Pharmacy, Inner Mongolia Medical University, Inner Mongolia 010110, China
  • Received:2017-04-07 Online:2017-09-10 Published:2017-09-10
  • Supported by:
    National Natural Science Foundation of China (No. 81260680), Youth Science and Technology Talent in High Schools of Inner Mongolia Autonomous Region of 2014 (No. NJYT-14-A12), Guidance Award Fund for Science and Technology Innovation in Inner Mongolia Autonomous Region of 2016, Innovation Repreneuriship Training Program of College Studants in Inner Mongolia Autonomous Region (No.201610132002)

摘要: 目的 建立蒙药复方述达格-4体内外特征图谱,归属并鉴定其体外化学成分及体内吸收入血成分。方法 取Wistar大鼠,灌胃蒙药复方述达格-4及其各单味药有效组分,分离制备含药血清,HPLC法测定,建立蒙药复方述达格-4体外及含药血清HPLC特征图谱,分析蒙药复方述达格-4各单味药材体外及其大鼠含药血清特征图谱,比对、归属并鉴定蒙药复方述达格-4体外化学成分及口服蒙药复方述达格-4后的大鼠吸收入血成分。结果 分析蒙药复方述达格-4体外HPLC特征图谱,共发现了64个峰。蒙药复方述达格-4灌胃给药后出现了23个入血成分,17个为体外成分直接入血,另外6个推测为新生代谢产物。蒙药复方述达格-4灌胃给药后出现的23个入血成分全部来源于各单味药材及全方,其中1,2,3,9,23号峰来源于高良姜;14号峰来源于木香;13号峰来源于石菖蒲;15,20,21,22号峰来源复杂,均来源于高良姜、木香、石菖蒲;6,10,17,18,19号峰来源于高良姜、石菖蒲;7,16号峰来源于高良姜、木香;11号峰来源木香、石菖蒲;4,5,8,12号峰来源于全方,并从中鉴定出7个蒙药复方述达格-4成分。结论 血中成分及代谢产物可能为蒙药复方述达格-4体内直接作用的有效成分,主要来源于蒙药复方述达格-4及其各单味药。

关键词: 蒙药述达格-4, 特征图谱, 化学成分, 血清, 大鼠

Abstract: Objective To establish in vivo and in vitro the characteristic chromatograms of Mongolian medicine compound formula-Shudage-4, and affiliate and identify in vitro chemical compositions of Shudage-4 and its compositions in vivo absorbed into blood. Methods Wistar rats were intragastrically given Shudage-4 and its different single medicinal, and then serum samples were prepared and determined by using high performance liquid chromatography (HPLC). The HPLC characteristic chromatograms of in vitro samples of Shudage-4 and its different single medicinal and Shudage-4 medicated serum were established and analyzed. The chemical compositions in vitro of Shudage-4 and its compositions in vivo absorbed into blood after taken by rats were compared, affiliated and identified. Results There were totally 64 peaks found after analyzing in vitro HPLC characteristic chromatograms of Shudage-4. There were 23 chemical compositions absorbed into blood, and 17 in vitro ones absorbed directly into blood and 6 speculated as new metabolites. These 23 chemical compositions were all derived from different were derived from Gaoliangjiang (Rhizoma Alpiniae Officinarum, Galangal Rhizome), peak 14, from Muxiang (Radix Aucklandiae, Common Aucklandia Root) and peak 13, from Shichangpu (Rhizoma Acori Tatarinowii, Grassleaf Sweetflag Rhizome). The peaks 15, 20, 21 and 22 were derived from Gaoliangjiang, Muxiang and Shichangpu, peaks 6, 10, 17, 18 and 19, from Gaoliangjiang and Shichangpu, peaks 7 and 16, from Gaoliangjiang and Muxiang, peak 11, from Muxiang and Shichangpu, and peaks 4, 5, 8 and 12, from the whole formula of Shudage-4, from which 7 chemical compositions were identified. Conclusion The chemical compositions and metabolites in blood may be the active principles of Shudage-4 with direct effects in vitro, which are derived respectively from different single medicinal in Shudage-4.

Key words: Mongolian medicine Shudage-4, specific chromatogram, chemical compositions, serum, rats

中图分类号: 

  • R284.1