主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

北京中医药大学学报 ›› 2017, Vol. 40 ›› Issue (9): 764-771.doi: 10.3969/j.issn.1006-2157.2017.09.010

• 中药化学 • 上一篇    下一篇

零陵香挥发性成分的SPME-GC/MS分析*

唐雪阳1,秦优1,4,陈林1,2,周融融1,邵湘宁4,施树云5,唐纯玉6,张水寒1,2,3#   

  1. 1 湖南省中医药研究院中药研究所 湖南 410013;
    2 湖南省中药原料质量监测技术服务中心;
    3 数字中医药湖南省高等学校2011协同创新中心;
    4 湖南中医药大学;
    5 中南大学;
    6 湖南时代阳光药业股份有限公司
  • 收稿日期:2017-03-02 出版日期:2017-09-10 发布日期:2017-09-10
  • 通讯作者: 张水寒,女,博士,教授,博士生导师,研究方向:中药资源及综合开发利用,E-mail:zhangshuihan0220@126.com
  • 作者简介:唐雪阳,女,硕士
  • 基金资助:
    *国家中医药管理局中医药公共卫生专项(No.财社[2011]-76),湖南省中医药研究院院级科研课题(No.201601)

Volatile constituents of Linglingxiang (Lysimachia foenum-graecum Hance, Holy Basil) analyzed by applying solid phase microextraction-gas chromatography-mass spectrometry*

TANG Xueyang1, QIN You1,4, CHEN Lin1,2, ZHOU Rongrong1, SHAO Xiangning4, SHI Shuyun5, TANG Chunyu6, ZHANG Shuihan1,2,3#   

  1. 1 Institute of Chinese Materia Medica, Hunan Academy of Chinese Medicine, Hunan 410013, China;
    2 Hunan Chinese Medicine Raw Material Quality Monitoring Technology Service Center, Hunan 410013, China;
    3 2011 Collaboration and Innovation Center for Digital Chinese Medicine in Hunan, Hunan 410208, China;
    4 Hunan University of Chinese Medicine, Hunan 410016, China;
    5 Central South University, Hunan 410083, China;
    6 Hunan Times Sunshine Pharmaceutical Corp., Hunan 425000, China
  • Received:2017-03-02 Online:2017-09-10 Published:2017-09-10
  • Supported by:
    Special Project of Public Health of State Adiministration of Traditional China Medicine(Financial Society, No. [2011]-76), Hunan Academy of Chinese Medicine (No. 201601)

摘要: 目的 采用固相微萃取-气相/质谱(SPME-GC/MS)联用技术分析零陵香挥发性成分。方法 利用 75 μm 碳分子筛/聚二甲基硅氧烷( 75 μm CAR/PDMS)、 65 μm 二乙烯基苯/聚二甲基硅氧烷( 65 μm DVB/PDMS)、 85 μm 聚丙烯酸酯( 85 μm PA)和 100 μm 聚二甲基硅氧烷(100 μm PDMS)共4种不同涂层的固相微萃取( SPME)萃取头,在最佳条件下,分别对零陵香挥发性成分进行萃取,并通过气相/质谱(GC/MS)联用分析检测其化学成分。结果 零陵香挥发性成分 SPME 关键参数为:萃取温度 80 ℃ 、时间 50 min 。 4 种萃取头共检出零陵香挥发性成分 10 类 103 种,酚类、酯类和烃类化合物是零陵香挥发性成分的主要化学类型。 4 种萃取头检出共有成分 6 种,分别为葫芦巴内酯、二氢猕猴桃内酯、(+)-雪松醇、植酮、菲、 3-氨基-4,5-二甲基-2(5H)-呋喃酮;各萃取头中 75 μm CAR/PDMS 萃取头检出挥发性成分数量最多。结论 本文首次采用 SPME-GC/MS 对零陵香挥发性成分进行测定。该方法简单、快速、准确,为零陵香物质基础研究提供参考借鉴。

关键词: 零陵香, 挥发性成分, 固相微萃取, 气相色谱-质谱联用

Abstract: Objective To analyze the volatile constituents of Linglingxiang (Lysimachia foenum-graecum, Hance, Holy Basil) by applying solid phase microextraction-gas chromatography-mass spectrometry (SPME-GC/MS). Methods The heads of solid phase microextraction (SPME) with 4 kinds of different coating, 75 μm CAR/PDMS, 65 μm DVB/PDMS, 85 μm PA and 100 μm PDMS, were used to extract the under the best extraction condition, and then these volatile constituents were detected by using gas chromatography-mass spectrometry (GC-MS). Results The key parameters of SPME for extracting volatile constituents from Linglingxiang were as follows: temperature was 85℃ and extracting time was for 50 min. A total of 10 types including 103 kinds of volatile constituents were identified by 4 different SPME extraction heads, and phenols, esters and hydrocarbons were main chemical types of volatile compounds in Linglingxiang. There were totally 6 volatile constituents detected by 4 different SPME extraction heads, and they were Sugarlactone, Dihydroactinidiolide, (+)-cedrol, phytone, phenanthrene, and 3-Amino-4, 5-dimethyl-2(5h)-furanone. Among all extraction heads, the head of 75 μm CAR/PDMS detected the largest number of volatile constituents reached to 43. Conclusion SPME-GC/MS was used for the first time form determining the volatile constituents in Linglingxiang. The method is simple, rapid and accurate, which can offer reference to further studies on the material base of Linglingxiang.

Key words: Linglingxiang (Lysimachia foenum-graecum Hance, Holy Basil), volatile constituents, solid phase microextraction, gas chromatography-mass spectrometry

中图分类号: 

  • R284.1