主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

北京中医药大学学报 ›› 2017, Vol. 40 ›› Issue (10): 872-875.doi: 10.3969/j.issn.1006-2157.2017.10.014

• 针灸 • 上一篇    下一篇

电针对慢性应激抑郁模型大鼠海马CXCR4和IP-10蛋白表达的影响*

梁星辰1,姜会梨1,张丹妹2,史榕荇3,王瑜1,李晶1,杨昕婧1,赵冰骢1,俞秋云1,李想1,冯世醒1,图娅1#   

  1. 1 北京中医药大学针灸推拿学院 北京 100029;
    2 郑州大学附属洛阳市中心医院;
    3 中日友好医院针灸科
  • 收稿日期:2016-11-21 出版日期:2017-10-10 发布日期:2017-10-10
  • 通讯作者: 图娅,女,博士,教授,主任医师,博士生导师,研究方向:针刺抗抑郁临床与机理研究,E-mail:tuyab@263.net
  • 作者简介:梁星辰,女,在读硕士生
  • 基金资助:
    *国家自然科学基金项目(No.81173334,No.81373729)

Effects of electro-acupuncture on hippocampal CXCR4 and IP-10 protein expression in rats with depression disorder*

LIANG Xingchen1, JIANG Huili1, ZHANG Danmei2, SHI Rongxing3, WANG Yu1, LI Jing1, YANG Xinjing1, ZHAO Bingcong1, YU Qiuyun1, LI Xiang1, FENG Shixing1, TU Ya1#   

  1. 1 School of Acupuncture-moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 100029, China;
    2 Luoyang Central Hospital Affiliated to Zhengzhou University, Henan 471000, China;
    3 China-Japan Friendship Hospital, Beijing 100029, China
  • Received:2016-11-21 Online:2017-10-10 Published:2017-10-10
  • Supported by:
    National Natural Science Foundation of China(No.81173334,No.81373729)

摘要: 目的 基于抗体蛋白芯片技术观察电针对慢性应激抑郁模型大鼠海马组织趋化因子受体-4(CXCR4)和趋化因子10(IP-10)表达的影响,探究电针抗抑郁的机制。方法 将40只SD大鼠随机平均分为正常组、模型组、电针组和氟西汀组。电针组于每日给予应激前1 h电针“百会”和“印堂”穴,氟西汀组于每日给予应激前1 h药物灌胃,均连续28 d。应用抗体蛋白芯片技术检测大鼠海马CXCR4和IP-10蛋白表达的变化,以倍数变化(fold change)来判断蛋白的差异表达。结果 与正常组比较,慢性不可预知性温和应激可使模型组大鼠海马CXCR4和趋化因子IP-10表达上调(fold change分别为1.21、1.50);电针和氟西汀可逆转模型大鼠海马CXCR4和IP-10的上调表达(电针组fold change分别为0.65、0.72;氟西汀组fold change分为0.57、0.62)。结论 电针对慢性应激抑郁状态的改善作用可能是通过调节海马CXCR4和IP-10的表达来实现的。

关键词: 电针, 抑郁症, 慢性应激, 趋化因子受体-4, 趋化因子10, 抗体蛋白芯片, 大鼠

Abstract: Objective To investigate the effect of electro-acupuncture (EA) on hippocampal chemokine cys-x-cys receptor 4 (CXCR4) and interferon-inducible protein-10 (IP-10) in rats with depression disorder. Methods The rat model of depression disorder was induced by unpredictable chronic-mild stimulation. Forty male rats were randomly divided into normal, model, EA and fluoxetine groups. Rats in EA group received electro-acupuncture at GV20 (Baihui) and GV29 (Yintang). Rats in fluoxetine group were administered with fluoxetine by oral gavage. On the 28th day, the expression of hippocampal CXCR4 and IP-10 were detected by using antibody microarrays technology. Fold change and ratio of the density of the proteins were used to investigate alteration of the differential protein expression. Results Compared with the normal group, unpredictable chronic-mild stimulation up-regulated the expression of hippocampal CXCR4 and IP-10 in model group (fold change=1.21,1.50). Compared with the model group, EA and fluoxetine reversed the up-regulation of hippocampal CXCR4 and IP-10 (EA group: fold change=0.65, 0.72; fluoxetine group: fold change=0.57, 0.62). Conclusion Effects of electro-acupuncture on depression disorder may be achieved by regulating the expression of hippocampal CXCR4 and IP-10.

Key words: electroacupuncture, depression disorder, chronic stress, CXCR4, IP-10, antibody microarrays technology, rats

中图分类号: 

  • R245.31