主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

北京中医药大学学报 ›› 2017, Vol. 40 ›› Issue (11): 923-927.doi: 10.3969/j.issn.1006-2157.2017.11.009

• 科技之窗 • 上一篇    下一篇

益气活血通络方对糖尿病周围神经病变小鼠JNK信号通路蛋白及炎性因子的影响*

姚永传1,申国明2#,江爱娟2,王靓2,叶树1,王柳1   

  1. 1 安徽中医药大学中西医结合临床学院 安徽 230038;
    2 安徽中医药大学研究生院
  • 收稿日期:2017-05-22 出版日期:2017-11-10 发布日期:2017-11-10
  • 通讯作者: 申国明,男,硕士,博士生导师,教授,主要研究方向:中医药防治糖尿病神经病变机理研究,E-mail:shengm_66@163.com
  • 作者简介:姚永传,男,在读硕士生
  • 基金资助:
    国家自然科学基金项目(No.81273646),安徽省自然科学基金项目(No.1608085MH232)

Influence of Yiqi Huoxue Tongluo Fang on JNK signal pathway and inflammatory factors in mice with diabetic peripheral neuropathy*

YAO Yongchuan1, SHEN Guoming2#, JIANG Aijuan2, WANG Liang2, YE Shu1, WANG Liu1   

  1. 1 Clinical School of Integrated Traditional Chinese and Western Medicine, Anhui University of Traditional Chinese Medicine, Anhui 230038, China;
    2 Graduate School, Anhui University of Traditional Chinese Medicine, Anhui 230038, China
  • Received:2017-05-22 Online:2017-11-10 Published:2017-11-10
  • Supported by:
    National Natural Science Foundation (No. 81273646), Natural Science Foundation of Anhui Province(No.1608085MH232)

摘要: 目的 研究益气活血通络方对糖尿病周围神经病变模型小鼠(db/db小鼠)JNK信号通路蛋白及炎性因子的影响,探讨其对db/db小鼠周围神经的保护作用机制。方法 40只db/db小鼠随机分为5组:模型组、硫辛酸组和益气活血通络方高、中、低剂量(6.4、3.2、1.6 g/kg)组,另设8只db/m小鼠作为正常组。灌胃给药,1次/d,连续8周。末次给药后,测定小鼠足底热敏反应时间和运动神经传导速度(MNCV);酶联免疫吸附法(ELISA法)检测血清中白细胞介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)水平;Western blot法检测背根神经节中c-jun氨基末端激酶(JNK)和磷酸化的c-jun氨基末端激酶(p-JNK)的蛋白表达。结果 与正常组比较,模型组小鼠MNCV减慢,足底热敏反应时间延长,血清中IL-1β、TNF-α水平升高,背根神经节中JNK、p-JNK蛋白的表达增强,差异具有统计学意义(P<0.01或P<0.05)。与模型组比较,益气活血通络方能提高小鼠MNCV,缩短足底热敏反应时间,降低血清中IL-1β、TNF-α水平,一定程度下调背根神经节中JNK、p-JNK的蛋白表达(P<0.01或P<0.05)。结论 益气活血通络方能抑制JNK信号转导通路的异常激活,减轻db/db小鼠炎症反应,从而保护周围神经的功能。

关键词: 益气活血通络方, db/db小鼠, 糖尿病周围神经病变, JNK信号转导通路, 炎性因子

Abstract: ObjectiveTo study the influence of Yiqi Huoxue Tongluo Fang (Qi-Replenishing Blood-Activating Collateral-freeing Decoction) on signal pathway of c-jun N-terminal kinase (JNK) and inflammatory factors in mice with diabetic peripheral neuropathy (db/db mice), and discuss its mechanism in protecting peripheral nerve. Methods The db/db mice (n=40) were randomly divided into 5 groups: model group, lipoic acid group, high-dose Yiqi Huoxue Tongluo Fang group (6.4 g/kg, high-dose group), mid-dose Yiqi Huoxue Tongluo Fang group (3.2 g/kg, mid-dose group) and low-dose Yiqi Huoxue Tongluo Fang group (1.6 g/kg, low-dose group), and other db/m mice (n=8) were chosen into normal group. All groups were intragastrically given corresponding drugs once a day for 8 weeks. After the last administration of drugs, the plantar thermal response time and motor nerve conduction velocity (MNCV) were detected. The levels of interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) were detected by using enzyme-linked immunosorbent assay (ELISA). The protein expressions of JNK and phosphorylase JNK (p-JNK) in dorsal root ganglion (DRG) were detected by using Western blot method. Results Compared with normal group, MNCV was slower, plantar thermal response time was longer, levels of IL-1β and TNF-α increased, and protein expressions of JNK and p-JNK are stronger in model group (P<0.01 or P<0.05). Compared with model group, Yiqi Huoxue Tongluo Fang promoted MNCV, shortened plantar thermal response time, decreased levels of IL-1β and TNF-α and down-regulated protein expressions of JNK and p-JNK to some extent (P<0.01 or P<0.05). ConclusionYiqi Huoxue Tongluo Fang can inhibit the abnormal activation of JNK signal pathway and relieve inflammatory reaction to protect peripheral nerve in db/db mice.

Key words: Yiqi Huoxue Tongluo Fang (Qi-Replenishing Blood-Activating Collateral-freeing Decoction), db/db mice, diabetic peripheral neuropathy, JNK signal pathway, inflammatory factors

中图分类号: 

  • R285.5