主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

北京中医药大学学报 ›› 2017, Vol. 40 ›› Issue (11): 946-953.doi: 10.3969/j.issn.1006-2157.2017.11.013

• 中药化学 • 上一篇    下一篇

基于高分辨液质联用技术的不同产地鲜丹参药材品质分析*

邵远洋1,徐国杰1,2,晋小雁1,刘思琦3,张弛1,王娟1,马双双1,张彩娟1,邱敏懿1,刘春生1,王学勇1#   

  1. 1 北京中医药大学中药学院 北京 100102;
    2 北京中医药大学生命科学学院;
    3 天津市河西区挂甲寺社区卫生服务中心
  • 收稿日期:2017-06-06 出版日期:2017-11-10 发布日期:2017-11-10
  • 通讯作者: 王学勇,男,博士,教授,博士生导师,主要研究方向:药用植物学与分子生药学研究,E-mail:wxyph.d@163.com
  • 作者简介:邵远洋,男,在读硕士生
  • 基金资助:
    国家自然科学基金项目(No.81274011),中央本级重大增减支项目(No.2060302),北京市自然科学基金项目(No.7152094)

Quality analysis on fresh Danshen (Radix Salviae Miltiorrhizae, Red Sage Root) from different producing areas based on technology of high-resolution liquid chromatography-mass spectrometry*

SHAO Yuanyang1, XU Guojie1,2, JIN Xiaoyan1, LIU Siqi3, ZHANG Chi1, WANG Juan1, MA Shuangshuang1, ZHANG Caijuan1, QIU Minyi1, LIU Chunsheng1, WANG Xueyong1#   

  1. 1 School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China;
    2 School of Life Sciences, Beijing University of Chinese Medicine, Beijing 100029, China;
    3 Health Service Center, Guajiasi Community, Hexi District, Tianjin 300210, China
  • Received:2017-06-06 Online:2017-11-10 Published:2017-11-10
  • Supported by:
    National Natural Science Foundation of China (No. 81274011), Central Major Adding and Removing Project (No. 2060302), Natural Science Foundation of Beijing (No. 7152094)

摘要: 目的 分析不同产地新鲜丹参中主要活性成分含量,合理评价鲜丹参药材品质。方法 采用LTQ-Orbitrap XL高分辨质谱仪测定全国7个不同产地新鲜丹参中5个丹酚酸类和12个丹参酮类成分。在此基础上,进行主成分分析(PCA)和最小二乘法分析(PLS)。结果 同一产地新鲜丹参主要活性成分的含量基本一致,不同产地新鲜丹参主要活性成分的含量差异显著;其中,湖北产区丹参的丹酚酸类成分含量最高,四川和河南产区丹参的丹参酮I和丹参酮IIA成分含量最高;PCA和PLS分析显示,大部分活性成分的含量分布具有明显的产地特异性。结论 不同产地新鲜丹参质量差异显著并具有较显著的产地特异性,以新鲜丹参药材作为品质评价材料,是现今基于干药材为品质评价原料研究方法的有益补充,为更全面综合的评价丹参及其他中药材探索了新的研究途径。

关键词: 丹参, 活性成分, 含量测定, 主成分分析, 最小二乘法分析, 差异性

Abstract: Objective To analyze the content of active components in fresh Danshen (Radix Salviae Miltiorrhizae, Red Sage Root) from different producing areas, and review reasonably the medicinal quality of fresh Danshen. Methods The content of 5 salvianolic acids and 12 tanshinones in fresh Danshen, from 7 different producing areas, were determined by applying LTQ-Orbitrap XL high-resolution mass spectrometer. On this basis, all content were analyzed by using principal component analysis (PCA) and partial least square (PLS) analysis. Results The content of active components in fresh Danshen from the same producing area were basically the same, and difference was significant in content of active components among fresh Danshen from different producing areas. The content of salvianolic acids was the highest in fresh Danshen from Hubei, and content of tanshinone I and tanshinone IIA were the highest in fresh Danshen from Sichuan and Henan. The analysis results of PCA and PLS showed that the distribution of most active components had a distinct regional specificity. Conclusion The difference is significant in quality of fresh Danshen from different producing areas, and they have a distinct regional specificity. Using fresh Danshen as quality evaluation materials, that is a useful supplement to review quality of raw medicinal materials based on dry herbs. It explored a new and more comprehensive approach for reviewing other Chinese medicinal materials.

Key words: Danshen (Radix Salviae Miltiorrhizae, Red Sage Root), content determination, principal component analysis, partial least square analysis, variation

中图分类号: 

  • R284.1