主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

北京中医药大学学报 ›› 2018, Vol. 41 ›› Issue (3): 210-215.doi: 10.3969/j.issn.1006-2157.2018.03.006

• 科技之窗 • 上一篇    下一篇

调脂通脉颗粒对ApoE基因敲除小鼠血脂、RLP-C、sICAM-1、sVCAM-1的影响*

李璐1, 郭杨志2, 杨惠民1#, 钱丽超1, 史成1, 王淑艳3, 廖磊4   

  1. 1 北京中医药大学东直门医院 北京 100070;
    2 首都医科大学附属北京世纪坛医院;
    3 北京中医药大学;
    4 北京市卫生局临床药学研究所
  • 收稿日期:2017-10-09 出版日期:2018-03-30 发布日期:2018-03-30
  • 通讯作者: # 杨惠民,男,硕士,教授,主任医师,博士生导师,研究方向: 中医药防治老年病,E-mail: yanghuimin012005@ 163.com
  • 作者简介:李璐,女,在读博士生
  • 基金资助:
    *国家自然科学基金项目(No.81503503)

Influence of Tiaozhi Tongmai Granule on blood fat, RLP-C, sICAM-1 and sVCAM-1 in ApoE-/- mice*

Li Lu1, Guo Yangzhi2, Yang Huimin1#, Qian Lichao1, Shi Cheng1, Wang shuyan3, Liao Lei4   

  1. 1 Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100070, China;
    2 Beijing Shijitan Hospital, Capital University of Medical Sciences, Beijing 100038, China;
    3 Beijing University of Chinese Medicine, Beijing 100029, China;
    4 Institute of Clinical Pharmacy, Beijing Municipal Health Bureau, Beijing 100053, China
  • Received:2017-10-09 Online:2018-03-30 Published:2018-03-30
  • Supported by:
    *National Natural Science Foundation of China (No. 81503503)

摘要: 目的 探讨调脂通脉颗粒对ApoE 基因敲除(ApoE-/-)小鼠血脂、富含甘油三酯脂蛋白残粒、细胞黏附分子的影响。方法 将30只ApoE-/-小鼠随机分为模型组、非诺贝特组及调脂通脉颗粒组,每组10只,另取10只C57BL/6J小鼠作为正常组。模型组及给药组小鼠以高脂饲料饲养,同时给予调脂通脉颗粒或非诺贝特,12周后测富含甘油三酯脂蛋白残粒(RLP-C)及血脂四项;采用ELISA 法检测血清细胞间黏附分子-1(sICAM-1)、血管细胞黏附分子-1(sVCAM-1)水平;取主动脉根部做石蜡切片,行HE染色观察形态学变化。结果 第12周,与模型组比较,调脂通脉颗粒组和非诺贝特组小鼠体重下降。与正常组比较,模型组、非诺贝特组、调脂通脉颗粒组血清RLP-C、TC、TG、HDL-C、LDL-C升高,差异有统计学意义(P<0.05);与模型组比较,非诺贝特组、调脂通脉颗粒组血清RLP-C、TG降低,差异有统计学意义(P<0.05)。与正常组比较,模型组血清sICAM-1、sVCAM-1水平升高,差异有统计学意义(P<0.05);与模型组比较,非诺贝特组、调脂通脉颗粒组血清sICAM-1、sVCAM-1水平降低,差异有统计学意义(P<0.05);非诺贝特组与调脂通脉颗粒组SVCAM-1与正常组比较,差异无统计学意义。HE染色表明模型组主动脉内膜增厚伴易损斑块形成,调脂通脉颗粒组内膜增厚伴稳定斑块。结论 调脂通脉颗粒可通过降低RLP-C的水平,减少细胞黏附分子表达,延缓动脉粥样硬化进展。

关键词: 血脂, 富含甘油三酯脂蛋白残粒, 动脉粥样硬化, ApoE基因敲除小鼠

Abstract: Objective To investigate the influence of Tiaozhi Tongmai (Fat-regulating and Vessel-freeing) Granule (TZTMG) on blood fat, triglyceride-rich remnant-like particle (RLP) and cell adhesion molecule in ApoE-/- mice. Methods After established model of atherosclerosis (AS), ApoE-/- mice (n=30) were randomly divided into model group, fenofibrate group and TZTMG group (each n=10). C57BL/6J mice (n=10) were chosen into normal group. All medicinal groups were fed with high-fat diet, meanwhile were intervened with TZTMG and fenofibrate. The indexes of triglyceride-rich RLP (RLP-C) and 4 items of blood fat were detected detector after 12 weeks. The levels of serum intercellular adhesion molecule-1 (ICAM-1) and vascular adhesion molecule-1 (VCAM-1) were detected by using ELISA. The aortic root was collected to prepare paraffin sections for observing morphological changes after HE staining. Results Since the 4th week, mouse weight decreased in TZTMG group and fenofibrate group compared with model group. The levels of serum RLP-C, TC, TG, HDL-C and LDL-C increased in model group, fenofibrate group and TZTMG group compared with normal group (P<0.05). The levels of serum RLP-C and TG decreased in fenofibrate group and TZTMG group compared with model group (P<0.05). The levels of ICAM-1 and VCAM-1 increased significantly in model group, fenofibrate group and TZTMG group compared with normal group (P<0.05). The levels of serum ICAM-1 and VCAM-1 decreased in fenofibrate group and TZTMG group compared with model group (P<0.05). Comparison in VCAM-1 among normal group, fenofibrate group and TZTMC group had no statistical significance. HE staining showed there were thickened aortic intima and vulnerable plaques in model group, and thickened aortic intima and stable plaques in TZTMG group. Conclusion TZTMG can decrease the expression of adhesion molecule and delay AS development through reducing RLP-C level.

Key words: blood fat, triglyceride-rich remnant-like particle, atherosclerosis, ApoE-/- mice

中图分类号: 

  • R285.5