主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

北京中医药大学学报 ›› 2018, Vol. 41 ›› Issue (4): 316-328.doi: 10.3969/j.issn.1006-2157.2018.04.010

• 中药化学 • 上一篇    下一篇

基于药物体系酚类特征架构的决明子质量表征关联分析研究*

刘璐1, 李倩2 , 周德勇1 , 孙道涵1 , 路东波1 , 孔静1 , 才秀芬3, 王梦林2, 武秋红1 , 李文霞1 , 李思潼1, 范圆圆1, 黄玉娟1 , 冯鑫1 , 石任兵1,4#   

  1. 1 北京中医药大学中药学院 国家中医药管理局中药经典名方有效物质发现重点实验室 北京 102488;
    2 首都医科大学中医药学院;
    3 北京信息科技大学;
    4 北京市教委中药质量控制技术工程中心
  • 收稿日期:2017-12-20 出版日期:2018-04-30 发布日期:2018-04-30
  • 通讯作者: 石任兵,男,博士,教授,博士生导师,主要研究方向:中药(复方)有效物质基础研究与药物创新,E-mail:shirb@126.com
  • 作者简介:刘璐,女,在读硕士生
  • 基金资助:
    *十二五国家科技支撑计划项目(No.2012BAI29B06),北京市教委共建科研基地建设项目,北京中医药大学重点学科开放课题(No.2013-2DXKKF-23)

Quality representation and correlation analytical study of Semen Cassiae based on phenolic characteristic architecture of medical system*

Liu Lu1, Li Qian2, Zhou Deyong1, Sun Daohan1, Lu Dongbo1, Kong Jing1, Cai Xiufen3, Wang Menglin2, Wu Qiuhong1, Li Wenxia1, Li Sitong1, Fan Yuanyuan1, Huang Yujuan1, Feng Xin1, Shi Renbing1,4#   

  1. 1 Key Unit of Exploring Effective Substances of Classical and Famous Formulas of State Administration of Traditional Chinese Medicine, School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China;
    2 Capital Medical University, School of Chinese Medicine, Beijing 100069, China;
    3 Beijing Information Science and Technology University 100192, China;
    4 Quality Control Technology and Engineering Center of Chinese Medicine, Beijing Municipal Commission of Education, Beijing 102488, China
  • Received:2017-12-20 Online:2018-04-30 Published:2018-04-30
  • Supported by:
    National Sci-Tech Plan of the 12th Five-Year Plan (No.2012BAI29B06), Scientific Projects of Beijing Municipal Commission of Education

摘要: 目的 建立基于药物体系的决明子药材特征图谱及其特征指标性成分质量表征关联分析与评价模式,以有效、精准地评价决明子药材质量。方法 采用所建立的HPLC-PDA法检测决明子药物体系构架的特征图谱,基于特征图谱中特征峰的梳理及所属化学类型对11批决明子药材进行质的表征,基于特征图谱中6个特征指标性成分和萘骈吡喃酮类、蒽醌类的含量对11批决明子药材进行量的表征,并基于基准药材将11批决明子药材质与量的表征结果分别进行关联性分析。结果 以批号10决明子为基准药材,决明子药材特征图谱中共含有12个特征峰,11批药材色谱图中均含有此12个特征峰;批号5、11、8、1、2指标成分含量总体较高,批号5、11、2、9、7与基准药材关联性最高,综合评价得出批号5、11、8、1、2、9、7优良度居前。结论 所建立的决明子药材中酚类成分的含量测定方法简便、准确,所构建的质量表征关联分析模式可用于分析决明子药材质量整体性与应用有效性,为决明子药材的质量控制及评价提供可靠方法学依据。

关键词: 决明子, 酚类, 特征图谱, 关联分析, 质量评价

Abstract: Objective To establish the quality representation and correlation analysis method and the content of characteristic ingredients based on spectrum of medicinal system of Juemingzi (Semen Cassiae), in order to evaluate the quality of Semen Cassiae decocting pieces effectively and accurately. Methods HPLC-PDA method was established to characterize the quality of spectrum of Semen Cassiae medicinal system architecture; quality of 11 batches of Semen Cassiae decocting pieces was characterized based on the number and chemical type of peaks on characteristic spectrum. The content of 6 characteristic index components, naphthopyrones and anthraquinones were characterized by indexes in characteristic spectrum. Correlation analysis of quality of Semen Cassiae was then performed with reference to the standard reference pieces. Results Taken as the reference substance, characteristic spectrum of batch 10 Semen Cassiae altogether contains 12 characteristic peaks, all characteristic peaks appeared on the chromatograms of 11 batches of Semen Cassiae. In 11 batches of Semen Cassiae, No.5, 11, 8, 1, 2 pieces contained higher effective index components, while No. 5, 11, 2, 9, and 7 pieces had high relevance with the reference piece (No.10). Consolidated quality characterization and correlation analysis, the quality of No. 5, 11, 8, 1, 2, 9, and 7 were excellent and dominant. Conclusion The HPLC method to analyze quality and quantity of phenolic components spectrum of 11 batches of Semen Cassiae is simple and accurate. The evaluation mode of quantity and quality and their correalation based on Semen Cassiae medical system, including systemic correlation and appilication validity, as it can provide a reliable methodological basis for the comprehensive quality evaluation of Semen Cassiae.

Key words: Semen Cassiae, phenolic, characteristic spectrum, correlation analysis, quality evaluation

中图分类号: 

  • R284.1