主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

北京中医药大学学报 ›› 2018, Vol. 41 ›› Issue (5): 383-394.doi: 10.3969/j.issn.1006-2157.2018.05.006

• 中药化学 • 上一篇    下一篇

沙棘酚类特征成分含量测定及其特征图谱质量表征关联分析研究*

张元元1, 吕珊1, 陈梦杰1, 孔静1, 孙仁弟2, 张媛媛2, 路东波1, 武秋红1, 李文霞1, 王梦林3, 范圆圆1, 冯鑫1, 李思潼1, 黄玉娟1, 姜艳艳1,4, 石任兵1,4#   

  1. 1 北京中医药大学中药学院 国家中医药管理局中药经典名方有效物质发现重点实验室北京 102488;
    2 上海绿谷生命园医药有限公司;
    3 首都医科大学中医药学院;
    4 北京市教委中药质量控制技术工程中心;
  • 收稿日期:2018-01-13 出版日期:2018-05-30 发布日期:2018-05-30
  • 通讯作者: # 石任兵,男,博士,教授,博士生导师,研究方向:中药(复方)有效物质基础研究与药物创新,E-mail:shirb@126.com
  • 作者简介:张元元,女,在读硕士生
  • 基金资助:
    *“十二五”国家科技支撑计划资助项目(No. 2012BAI29B06),北京市教委共建科研基地建设资助项目,北京中医药大学重点学科开放课题资助项目(No.2013-2DXKKF-23)

Quantitative determination of phenolic characteristic components and their quality representation and correlation analysis of specific chromatograms in Shaji*

Zhang Yuanyuan1, Lyu Shan1, Chen Mengjie1, Kong Jing1, Sun Rendi2, Zhang Yuanyuan2, Lu Dongbo1, Wu Qiuhong1, Li Wenxia1, Wang Menglin3, Fan Yuanyuan1, Feng Xin1, Li Sitong1, Huang Yujuan1, Jiang Yanyan1,4, Shi Renbing1,4#
  

  1. 1 Key Laboratory of Exploring Effective Substances of Classical and Famous Formulas of State Administration of Traditional Chinese Medicine, School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China;
    2 Shanghai GreenValley Shengmingyuan Pharmaceutical Co., Ltd, Shanghai 201700, China;
    3 School of Chinese Medicine, Capital University of Medical Sciences, Beijing 100069, China;
    4 Quality Control Technology and Engineering Center of Chinese Medicine, Beijing Municipal Commission of Education, Beijing 102488, China;
  • Received:2018-01-13 Online:2018-05-30 Published:2018-05-30
  • Supported by:
    National Sci-Tech Plan of the 12th Five-Year Plan (No. 2012BAI29B06)

摘要: 目的 建立沙棘酚类特征成分含量测定及其酚类特征图谱质量表征关联分析方法,应用关联分析评价模式,以有效、精准地评价沙棘药材质量。方法 采用二极管阵列检测器液相色谱法(HPLC-PDA)对11批沙棘药材中的酚类特征成分(原儿茶酸、鞣花酸、水仙苷、槲皮素、异鼠李素)进行含量测定,建立沙棘酚类特征图谱,基于特征图谱中特征峰的梳理及所属化学类型对11批沙棘药材进行质的表征,基于特征图谱中特征成分原儿茶酸、鞣花酸、水仙苷、槲皮素、异鼠李素及酚酸类(以原儿茶酸表征)、黄酮类(以水仙苷表征)的含量和峰面积分别对11批沙棘药材进行量的表征,并基于基准药材对11批沙棘药材质与量的表征结果进行关联性分析。结果 原儿茶酸、鞣花酸、水仙苷、槲皮素、异鼠李素等特征成分均具有良好的线性关系,方法学考察符合定量要求。以批号7为基准药材,在沙棘酚类特征图谱中共含有18个特征峰,其中酚酸类成分特征峰3个,黄酮类成分特征峰15个,11批药材色谱图中均含有此18个特征峰。采用质量表征关联分析与评价模式,得出批号8、7、10、4、5、11、1特征成分含量总体较高,批号6、2、3、1、11与基准药材关联性最高,综合评价得出批号1、11、8、6、7优良度居前。结论 建立的沙棘酚类特征成分含量测定方法简便、准确,构建的沙棘酚类特征图谱质量表征关联分析模式用于分析沙棘药材质量与应用有效性,可有效精准地评价沙棘质量。

关键词: 沙棘, 酚类, 特征图谱, 关联分析, 质量评价

Abstract: Objective To establish an analysis method for quantitatively determining phenolic characteristic components and correlation analyzing their quality representation of specific chromatograms in Shaji (Seabuckthorn Fruit, Hippophae rhamnoides), and to review the quality of Shaji effectively and accurately by applying association analysis-reviewing mode. Methods HPLC-PDA method was used to quantitatively determine the content of phenolic characteristic components in 11 batches of Shaji (protocatechuic acid, ellagic acid, narcissin, quercetin, isorhamnetin), and to establish phenolic specific chromatograms of Shaji. The quality of 11 batches of Shaji was characterized based on teasing characteristic peaks and chemical types. The quantity of 11 batches of Shaji was characterized based on quantity and peak areas of protocatechuic acid, ellagic acid, narcissin, quercetin, isorhamnetin, phenolic acids (represented by protocatechuic acid) and flavonoids (represented by narcissin) in the specific chromatograms. The characterized results of quality and quantity of 11 batches of Shaji were given association analysis based on baseline material of Shaji. Results The characteristic components of protocatechuic acid, ellagic acid, narcissin, quercetin, isorhamnetin all had good linear correlation, and the results of methodological investigation were in accordance with the quantitative determination requirements. Taken batch 7 of Shaji as baseline material, there were totally 18 characteristic peaks in phenolic specific chromatograms of Shaji, including 3 peaks of phenolic acids and 15 peaks of flavonoids, and all 18 characteristic peaks appeared in the chromatograms of 11 batches of Shaji. The quantity of characteristic components were higher in batch 8, 7, 10, 4, 5, 11 and 1 after analyzed and reviewed by using association analysis-reviewing mode. The relevance of batch 6, 2, 3, 1 and 11 was the highest with baseline material of Shaji. Comprehensive reviewing showed that the excellent extents of batch 1, 11, 8, 6 and 7 were prior. Conclusion The quantitative determination method of phenolic characteristic components in Shaji established in this study is easy and accurate. The association analysis-reviewing mode for quality characterization of phenolic specific chromatograms can be used for analyzing the quality and application validity of Shaji and reviewing quality of Shaji effectively and accurately.

Key words: Shaji (Seabuckthorn Fruit, Hippophae rhamnoides), phenols, specific chromatograms, correlation analysis, quality review

中图分类号: 

  • R284.1