主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

北京中医药大学学报 ›› 2018, Vol. 41 ›› Issue (6): 476-481.doi: 10.3969/j.issn.1006-2157.2018.06.007

• 科技之窗 • 上一篇    下一篇

姜黄素对大鼠肾间质成纤维细胞中小分子RNA-21及纤维化相关因子表达的影响*

刘祎1, 王曦廷1, 张澜1, 孙雪娇1, 韩森1, 唐磊2, 李亚东2, 李彧1#   

  1. 1 北京中医药大学中医学院 北京 100029;
    2 北京中医药大学生命科学学院
  • 收稿日期:2018-01-09 出版日期:2018-06-30 发布日期:2018-06-30
  • 通讯作者: 李彧,女,博士,研究员,硕士生导师,研究方向:中医药防治器官纤维化,E-mail:liyubeijing1973@163.com
  • 作者简介:刘祎,女,在读硕士生
  • 基金资助:
    *国家自然科学基金资助项目(No.81573716)

Effects of curcumin on the expression of microRNA-21 and fibrosis-related factors in rat renal interstitial fibroblast cells*

Liu Yi1, Wang Xiting1, Zhang Lan1, Sun Xuejiao1, Han Sen1, Tang Lei2, Li Yadong2, Li Yu1#   

  1. 1 School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China;
    2 School of Life Science, Beijing University of Chinese Medicine, Beijing 102488, China
  • Received:2018-01-09 Online:2018-06-30 Published:2018-06-30
  • Supported by:
    National Natural Science Foundation of China (No. 81573716)

摘要: 目的 探讨姜黄素对转化生长因子β1(TGF-β1)诱导的大鼠肾间质成纤维细胞(NRK49F)中小分子RNA-21(miR-21)及纤维化相关因子表达的影响。方法 将NRK49F细胞分为正常组、模型组、姜黄素组。模型组以TGF-β1(10 μg/L)诱导NRK49F细胞活化,姜黄素组以高、中、低浓度(20、10、5 μmol/L)干预24 h和72 h。免疫荧光方法及高内涵成像分析设备检测并分析NRK49F细胞中α-平滑肌肌动蛋白(α-SMA)表达的变化;RT-PCR方法检测NRK49F细胞中miR-21及纤维化相关因子COL1A1、COL3A1、α-SMA、Smad3 mRNA表达的变化。结果 TGF-β1作用于NRK49F细胞72 h后,能明显上调细胞中α-SMA蛋白的表达(P<0.01);与模型组相比,姜黄素各组的α-SMA蛋白表达明显下调(P<0.01、P<0.01、P<0.05)。TGF-β1作用于NRK49F细胞24 h后,能明显上调细胞中miR-21、COL1A1、COL3A1、α-SMA、Smad3 mRNA的表达(P<0.01);与模型组相比,姜黄素高、中浓度组可明显抑制miR-21、COL1A1、COL3A1、α-SMA、Smad3 mRNA的表达(P<0.01或P<0.05);姜黄素低浓度组可明显抑制COL1A1、COL3A1、α-SMA mRNA的表达(P<0.01),对miR-21、smad3 mRNA的表达有下调的趋势。结论 姜黄素能够有效抑制TGF-β1诱导的NRK49F细胞中纤维化相关因子的基因及蛋白表达,其作用与下调miR-21的表达相关。

关键词: 小分子RNA-21, 姜黄素, 肾脏纤维化, 转化生长因子β1, 大鼠肾间质成纤维细胞

Abstract: Objective To investigate the effects of curcumin on expression of microRNA-21 and fibrosis-related factors induced by transforming growth factor-beta 1(TGF-β1) in the renal interstitial fibroblast cells (NRK49F) of rats. Methods NRK49F cells were divided into normal group, model group, and curcumin groups. NRK49F cells in the model group were activated by TGF-β1(10μg/L). Cells in the curcumin groups were treated with curcumin at high, medium, and low concentration(20,10,5μmol/L respectively) for 24 and 72 hours. The protein expression of α-SMA were measured by using immunofluorescence cell staining and cell analyzer. The expression of micro RNA-21 and mRNA of fibrosis-related factors (COL1A1, COL1A2, COL3A1, ACTA2, Smad3) were measured with RT-PCR. Results After treated with TGF-β1 for 72 hours, the protein expression level of α-SMA increased significantly(P<0.01);Compared with TGF-β1-treated group, the protein expression level of α-SMA decreased significantly in the curcumin groups (20,10,5 μmol/L)(P<0.01, P<0.01, P<0.05). After treated with TGF-β1 for 24 hours, the expression level of miR-21, COL1A1, COL3A1, α-SMA, and Smad3 mRNA increased significantly (P<0.01). Compared with TGF-β1-treated group, the expression level of miR-21, COL1a1, COL3a1, α-SMA, and Smad3 mRNA decreased significantly in the curcumin groups (20,10 μmol/L)(P<0.01, P<0.05);Compared with TGF-β1-treated group, the expression level of COL1A1, COL3A1, and α-SMA mRNA decreased significantly (P<0.01) and the expression level of miR-21 and smad3 mRNA showed a downward trend in the 5 μmol/L curcumin group. Conclusion Curcumin down-regulated fibrosis-related gene and protein expression induced by TGF-β1 in rat renal interstitial fibroblasts, which may be related to its down-regulating the expression of miR-21 genes.

Key words: microRNA-21, curcumin, renal fibrosis, TGF-β1, rat renal interstitial fitroblast cells

中图分类号: 

  • R285.5