主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

北京中医药大学学报 ›› 2018, Vol. 41 ›› Issue (7): 553-558.doi: 10.3969/j.issn.1006-2157.2018.07.005

• 博士之光 • 上一篇    下一篇

芪杉方联合顺铂对肺癌A549细胞PI3K/AKt信号通路的影响*

隋雨桐, 迟文成, 姜家康#   

  1. 黑龙江中医药大学附属第一医院 黑龙江 150040
  • 收稿日期:2017-12-11 出版日期:2018-07-30 发布日期:2018-07-30
  • 通讯作者: 姜家康,男,博士,主任医师,教授,硕士生导师,研究方向:中西医结合治疗肿瘤
  • 作者简介:隋雨桐,男,博士,住院医师
  • 基金资助:
    黑龙江自然科学基金资助项目(No.H201478)

Influence of Qishan Fang combined with cisplatin on PI3K/AKt signal pathway of lung cancer A549 cells*

Sui Yutong, Chi Wencheng, Jiang Jiakang#   

  1. First Affiliated Hospital of Heilongjiang University of Chinese Medicine, Heilongjiang 150040, China
  • Received:2017-12-11 Online:2018-07-30 Published:2018-07-30
  • Supported by:
    Natural Science Foundation of Heilongjiang (No. H201478)

摘要: 目的 观察芪杉方联合顺铂对肺癌A549细胞PI3K/AKt信号通路的影响。方法 Wisatr雄性大鼠随机分成芪杉方含药血清组、顺铂组、芪杉方含药血清联合顺铂组和空白对照组,每组10只,各组连续灌胃3 d后取血。分别运用MTT法、Annexin V-PE/7-AAD双染法、蛋白质印迹(Western blot)法、逆转录-聚合酶链反应(RT-PCR)法检测芪杉方含药血清联合顺铂对A549细胞增殖、细胞凋亡及其相关蛋白和基因表达的影响。结果 芪杉方含药血清组、顺铂组、芪杉方含药血清联合顺铂组分别作用于A549细胞时,均表现为抑制细胞增殖作用,其抑制率分别为27.20%(P<0.01),28.52%(P<0.01)和51.25%(P<0.01)。芪杉方含药血清组、顺铂组、芪杉方含药血清联合顺铂组Caspase-3、Caspase-9、mTOR及PI3K蛋白和基因表达量与空白对照组相比,表达量降低(P<0.01),且芪杉方含药血清联合顺铂组对A549细胞凋亡相关蛋白表达的影响最显著(P<0.01)。结论 芪杉方含药血清组、顺铂组、芪杉方含药血清联合顺铂组均能够抑制A549细胞增殖,激活Caspase-3、Caspase-9、AKT、mTOR相关凋亡途径,从而诱导A549细胞凋亡。

关键词: 芪杉方, 肺癌, 顺铂, PI3K/AKt信号通路

Abstract: Objective To observe the influence of Qishan Fang (Milkvetch Root and Taxus Chinensis Decoction) combined with cisplatin on PI3K/AKt signal pathway of lung cancer A549 cells. Methods Male Wisatr rats were randomly divided into group of Qishan Fang medicated serum (serum group), cisplatin group, medicated serum combined with cisplatin group (combined group) and blank control group (control group, each n=10). The serum samples were collected after all groups were orally given corresponding medicinal for 3 d. The influence of Qishan Fang medicated serum combined with cisplatin on proliferation, apoptosis and relative protein and genetic expressions of A549 cells were detected respectively by using methyl thiazolyl tetrazolium test (MTT), Annexin V-PE/7-AAD double-staining, Western blotting assay and real-time fluorescence quantitative polymerase chain reaction (RT-PCR). Results The inhibition of A549 cell proliferation was observed in serum group, cisplatin and combined group, and the inhibitory rate was, respectively 27.20% (P<0.01), 28.52% (P<0.01) and 51.25% (P<0.01). The protein and genetic expressions of Caspase-3, Caspase-9, mTOR and PI3K decreased in serum group, cisplatin and combined group compared with control group (P<0.01). The influence on protein expression related to A549 cell apoptosis was the most significant in combined group (P<0.01). Conclusion The proliferation of A549 cell can be inhibited, relative apoptosis pathways of Caspase-3, Caspase-9, AKT and mTOR can be activated in serum group, cisplatin group and combined group, and apoptosis of A549 cell can be induced. Qishan Fang combined with cisplatin has better regulative effect than single of them, and inhibitions of proliferation rate and apoptosis rate are significantly improved in combined group than those in cisplatin group and serum group.

Key words: Qishan Fang (Milkvetch Root and Taxus Chinensis Decoction), lung cancer, cisplatin, PI3KAKt signal pathway, combined medication, rats

中图分类号: 

  • R285.5