主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

北京中医药大学学报 ›› 2018, Vol. 41 ›› Issue (7): 567-571.doi: 10.3969/j.issn.1006-2157.2018.07.007

• 科技之窗 • 上一篇    下一篇

金匮肾气丸加味膏、汤方干预肾阳虚模型大鼠的药效学对比研究*

郑龙飞, 韩玲, 郝宇, 高治理, 石翎笙, 贺娟#   

  1. 北京中医药大学中医学院 北京 100029
  • 收稿日期:2018-01-10 出版日期:2018-07-30 发布日期:2018-07-30
  • 通讯作者: 贺娟,女,博士,教授,博士生导师,研究方向: 中医藏象理论、五运六气学说,E-mail: hejuan6428@ sina.com
  • 作者简介:郑龙飞,男,在读硕士生
  • 基金资助:
    国家自然科学基金资助项目(No.81273874),北京中医药大学新奥奖励基金项目 (No. 2015-XAJLJJ-003)

Comparison in pharmacodynamics between modified paste and decoction of Jinhui Shenqi Pill in rat model of kidney yang deficiency*

Zheng Longfei, Han Ling, Hao Yu, Gao Zhili, Shi Lingsheng, He Juan#   

  1. School of Traditional Chinese Medicine, Beijing University of Chinese Medicion, Beijing 100029, China
  • Received:2018-01-10 Online:2018-07-30 Published:2018-07-30
  • Supported by:
    National Natural Science Foundation of China (No.81273874)

摘要: 目的 对比研究金匮肾气丸加味膏方和汤方干预肾阳虚模型的疗效。方法 将40只SD雄性大鼠分为正常组、模型组、金匮肾气丸加味膏方组(简称肾气丸膏方组)、金匮肾气丸加味汤方组(简称肾气丸汤方组),除正常组以外各组通过采用按200 mg/kg剂量灌服腺嘌呤4周的方法复制肾阳虚证大鼠模型,肾气丸膏方组和肾气丸汤方组分别给予18.36 g/(kg·d)的金匮肾气丸加味膏方和汤方,持续干预6周。第6周末,腹主动脉抽取各组大鼠动脉血,取血清,检测各组大鼠下丘脑-垂体-性腺轴激素如睾酮(T)、雌二醇(E2) 、黄体生成素(LH)、人绒毛膜促性腺激素(HCG),下丘脑-垂体-肾上腺轴激素如促肾上腺皮质激素(ACTH)、皮质醇(COR)、17-羟皮质类固醇(17-OH-CS),下丘脑-垂体-甲状腺轴激素如三碘甲状腺原氨酸(T3)、四碘甲状腺原氨酸(T4)的含量。结果 与正常组相比,模型组LH含量显著下降(P<0.05),肾气丸膏方组与肾气丸汤方组LH皆显著升高(P<0.05)。与正常组相比,模型组E2含量显著下降(P<0.05),肾气丸汤方组E2较模型组显著升高(P<0.05),且肾气丸汤方组E2含量显著高于膏方组(P<0.05)。与正常组相比,模型组T有下降的趋势,但无显著性差异,肾气丸膏方组和肾气丸汤方组T较模型组显著升高(P<0.05)。与正常组相比,模型组17-OH-CS含量显著升高(P<0.05),肾气丸汤方组较模型组含量显著下降(P<0.05),与肾气丸膏方组相比,肾气丸汤方组17-OH-CS含量降低显著(P<0.05)。与正常组相比,模型组T4含量显著下降(P<0.05),与模型组相比,肾气丸膏方组T4含量显著升高(P<0.05),与肾气丸汤方组相比,肾气丸膏方组T4含量显著升高(P<0.05)。结论 金匮肾气丸加味膏方和汤方两种剂型均可以通过提高促LH和T含量起到温补肾阳的功效,但是两种剂型又有差异,汤剂可以通过提高E2含量和降低17-OH-CS起到温补肾阳的功效,而膏剂则对此两指标无显著影响;膏剂可以通过提高T4水平起到温补肾阳功效,而汤剂对此指标则无显著影响。

关键词: 肾阳虚证, 金匮肾气丸, 剂型, 膏方, 大鼠

Abstract: Objective To compare and study the curative effects of modified paste and decoction of Jinhui Shenqi (Kidney-qi Pill in Synopsis of the Golden Chamber) Pill in intervention of rat model of kidney yang deficiency. Methods Male SD rats (n=40) were divided into normal group, model group, group of Jinhui Shenqi Pill paste (paste group) and group of Jinhui Shenqi Pill decoction (decoction group). Expect of normal group, other groups were orally given adenine (200 mg/kg) for 4 weeks for copying rat model of kidney yang deficiency pattern. The paste group and decoction group were respectively given modified paste or decoction of Jinhui Shenqi Pill for 6 weeks. The model group was orally given distilled water and ginseng group was given conventional breeding. On the end of the 6th week, the serum samples were collected from all groups for detecting the hormones of hypothalamus-pituitary-gonadal axis including testosterone (T), estradiol (E2), luteinizing hormone (LH) and human chorionic gonadotropin (HCG), hormones of hypothalamus-pituitary-adrenal axis including adrenocorticotrophic hormone (ACTH), cortisol (COR), 17-hydroxycorticosteroid (17-OH-CS), and hormones of hypothalamus-pituitary-thyroid axis including tri-iodothyronine (T3) and tetra-iodothyronine (T4). Results The content of LH decreased significantly in model group compared with normal group (P<0.05), and increased significantly in paste group and decoction group (P<0.05) but there was no significant difference between paste group and decoction group. The content of E2 decreased significantly in model group compared with normal group (P<0.05), increased significantly in decoction group compared with model group (P<0.05). There was no significant difference in content of E2 between paste group and model group, but it was significantly higher in decoction group than that in paste group (P<0.05). Compared with normal group, content of T had descending trend without significant difference. The content of T increased significantly in paste group and decoction group compared with model group (P<0.05), while there was no significant difference between paste group and decoction group. The content of 17-OH-CS increased significantly in model group compared with normal group (P<0.05), decreased significantly in decoction group compared with model group (P<0.05), and decreased significantly in decoction group compared with paste group (P<0.05). The content of T4 decreased significantly model group compared with normal group (P<0.05), increased significantly in paste group compared with model group (P<0.05) and increased significantly in paste group compared with decoction group (P<0.05). Conclusion The two types of preparation forms of Jinhui Shenqi Pill (paste and decoction) can play a role of warm tonification of kidney yang through increasing content of LH and T, but there is difference between these two types of preparation forms. The decoction of Jinhui Shenqi Pill can warmly tonify kidney yang through increasing content of E2 and decreasing content of 17-OH-CS, but paste of Jinhui Shenqi Pill has no significant effect on these two indexes. The paste of Jinhui Shenqi Pill can warmly tonify kidney yang through increasing content of T4, while decoction of Jinhui Shenqi Pill has no significant effect on this index.

Key words: kidney yang deficiency pattern, Jinhui Shenqi (Kidney-qi Pill in Synopsis of the Golden Chamber) Pill, preparation forms, paste preparation, rats

中图分类号: 

  • R285.5