主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

北京中医药大学学报 ›› 2018, Vol. 41 ›› Issue (8): 655-662.doi: 10.3969/j.issn.1006-2157.2018.08.007

• 博士之光 • 上一篇    下一篇

复方钩藤降压片对高血压大鼠左心室肥厚干预的代谢组学研究*

简维雄, 肖隋熙, 刘培, 罗颖, 陈偶英, 谭元生#   

  1. 湖南中医药大学国家重点学科中医诊断学 湖南省重点实验室 数字中医药协同创新中心 湖南 410208
  • 收稿日期:2017-09-22 出版日期:2018-08-30 发布日期:2018-08-30
  • 通讯作者: 谭元生,男,博士,教授,研究方向:中药药理与创新药物的研究,E-mail: tys702@126.com
  • 作者简介:简维雄,男,博士,副教授
  • 基金资助:
    国家自然科学基金资助项目(No.81774207,No.81202647,No.81473616),中医诊断国家重点学科开放项目

Metabonomic study of compound gouteng antihypertensive tablet's effects on left ventricular hypertrophy in hypertensive rats*

Jian Weixiong, Xiao Suixi, Liu Pei, Luo Ying, Chen Ouying, Tan Yuansheng#   

  1. National Key Discipline of TCM Diagnositics, Hunan Provincial Key Laboratory, Digital TCM Collaborative Innovative Innovative Center Hunan University of Chinese Medicine, Hunan 410208, China
  • Received:2017-09-22 Online:2018-08-30 Published:2018-08-30
  • Supported by:
    National Natural Science Foundation of China (No. 81774207, No.81202647, No.81473616), State Key Discipline Open Project of TCM diagnostics

摘要: 目的 研究复方钩藤降压片对高血压大鼠左心室肥厚干预的代谢产物变化及其机制。方法 24只自发性高血压大鼠随机平均分为模型组、复方钩藤降压片组、替米沙坦组(每组8只),另外选取Wistar-Kyoto (WKY)大鼠8只为空白对照组。采用气相色谱-质谱(GC-MS)检测技术,对左心室心肌组织代谢产物进行检测分析;应用偏最小二乘法(PLS)分析差异代谢产物谱。依据变量重要性(VIP)、自变量系数研究4组大鼠左心室代谢产物。结果 模型组与复方钩藤降压片组间自变量系数图显示:与复方钩藤降压片组正相关的是胞嘧啶核苷酸、腐胺、琥珀酸、D-甘油酸、果糖、肌苷、羊毛甾烯醇、抗坏血酸、谷氨酸、1,5-脱水葡萄糖醇;负相关的是乳酸、3-磷酸甘油酸、核糖、木糖醇、脯氨酸。复方钩藤降压片组与替米沙坦组间自变量系数图显示:与复方钩藤降压片组正相关的是腐胺、胞嘧啶核苷酸、甘油酸、来苏糖酸、二十四烷酸、肌醇、肌苷;负相关的是木糖醇、山梨糖醇、核糖、乳酸。结论 复方钩藤降压片可能对细胞结构调整、能量优化、氧化应激反应等机制更具有特异性。

关键词: 复方钩藤降压片, 左心室肥厚, 代谢组学, 自发性高血压大鼠

Abstract: Objective To study the metabolite changes and mechanism of compound gouteng (uncaria) anti-hypertensive tablet in the treatment of left ventricular hypertrophy. Methods 24 rats with spontaneously hypertension were randomly divided into the model group, compound gouteng antihypertensive tablet(CGTA) group and telmisartan(TMST) group. WKY (Wistar-Kyoto) rats were chosen as the control group. Metabolites were detected and analyzed by using gas chromatography-mass spectrometry (GC-MS), and the differential metabolite profiles of left ventricular myocardium were analyzed with partial least squares (PLS). Based on the importance of independent variables (VIP) and the coefficient of independent variable, the left ventricular metabolites of the four groups were investigated. Results The variable mapping of SHR group and CGTA group showed positive correlations with CGTA group were shown in cytosine nucleotide, putrescine, succinic acid, D-acid, glycerol fructose, inosine, dihydrolanosterol, ascorbic acid, glutamic acid, and 1,5-ag; and that negative correlations were found in lactic acid, 3-phosphoglycerate, ribose, xylitol, and proline. The variable maps of CGTA group and TMST group showed positive correlation with CGTA group were identified in putrescine, cytosine nucleotide acid, glycerol, lyxonic acid, twenty-four acid, inositol, and inosine; negative correlation were present in xylitol, sorbitol, ribose, and lactic acid. Conclusion The compound gouteng antihypertensive tablet may have specific effects on cell structure, energy optimization and oxidative stress reaction.

Key words: compound uncaria anti-hypertensive tablet, left ventricular hypertrophy, metabonomics, spontaneous hypertensive rats

中图分类号: 

  • R285.5