主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

北京中医药大学学报 ›› 2018, Vol. 41 ›› Issue (9): 787-792.doi: 10.3969/j.issn.1006-2157.2018.09.013

• 临床研究 • 上一篇    

基于核磁共振氢谱的慢性复发型溃疡性结肠炎中医虚、实证候血浆代谢组学研究*

王佳婕1, 曹云2, 李中峰3, 陈建新4, 王亮5, 黄佳钦2, 符欣2, 张忠绵2, 李志红2#   

  1. 1 云南省中医医院 云南 650021;
    2 北京中医药大学东直门医院;
    3 首都师范大学化学系;
    4 北京中医药大学;
    5 北京市怀柔区中医医院
  • 收稿日期:2018-05-02 出版日期:2018-09-30 发布日期:2018-09-30
  • 通讯作者: 李志红,男,博士,主任医师,硕士生导师,主要研究方向:中医药干预慢性胃肠道癌前病变的基础与临床,E-mail: lzhls1966@
    126.com
  • 作者简介:王佳婕,女,硕士,住院医师
  • 基金资助:
    国家重点研发计划资助项目 (No. 2017YFC1700106),吴阶平医学基金会临床科研专项资助项目(No.320.6750.17233)

Plasma metabolomic study on deficiency or excessiveness patterns of chronic recurrent ulcerative colitis based on H-nuclear magnetic resonance*

Wang Jiajie1, Cao Yun2, Li Zhongfeng3, Chen Jianxin4, Wang Liang5, Huang Jiaqin2, Fu Xin2, Zhang Zhongmian2, Li Zhihong2#   

  1. 1 Yunnan Provincial Hospital of Traditional Chinese Medicine, Yunnan 650021, China;
    2 Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China;
    3 Department of Chemistry, Capital Normal University, Beijing 100048, China;
    4 Beijing University of Chinese Medicine, Beijing 100029, China;
    5 Hospital of Traditional Chinese Medicine of Beijing Huairou District, Beijing 101400, China
  • Received:2018-05-02 Online:2018-09-30 Published:2018-09-30
  • Supported by:
    National Key Research and Development Program of China (No. 2017YFC1700106), Wu jieping Medical Foundation Funded Special Clinical Research Projects (No.320.6750.17233)

摘要: 目的 探讨慢性复发型溃疡性结肠炎(UC)虚、实证患者血浆代谢组学特征。方法 收集UC慢性复发型脾胃气虚证和大肠湿热证患者共31例及40例健康志愿者,观察两组患者基本情况,运用核磁共振氢谱(1H-NMR)定量检测血浆代谢物含量,采用主成分分析法(PCA)、偏最小二乘-判别分析法(PLS-DA)及正交偏最小二乘-判别分析法(OPLS-DA)进行多元统计分析。结果 筛选出潜在生物标志物10种,其中UC患者乙酰乙酸、丙酮水平高于健康人,乳酸、肌肽、丙氨酸、异亮氨酸则显著低于后者。慢性复发型UC大肠湿热证患者葡萄糖、苏氨酸、肌肽高于脾胃气虚证患者;缬氨酸、丙氨酸、甘氨酸低于脾胃气虚证患者。结论 慢性复发型UC虚、实证患者存在较为明显的糖代谢、氨基酸代谢及脂代谢紊乱,UC辨证分型具有代谢物质基础。

关键词: 溃疡性结肠炎, 代谢组学, 核磁共振氢谱, 大肠湿热证, 脾胃气虚证

Abstract: Objective To discuss the plasma metabolomic characteristics in patients with ulcerative colitis (UC) and deficiency or excessiveness patterns. Methods UC patients (n=31, UC group) with spleen-stomach qi deficiency pattern or large intestinal dampness-heat pattern and health volunteers (n=40, control group) were chosen, and general conditions were observed in 2 groups. And UC group was divided into subgroup of large intestinal dampness-heat pattern (subgroup A) and subgroup of spleen-stomach qi deficiency pattern (subgroup B). The content of plasma metabolites was determined quantitatively by using H-nuclear magnetic resonance (1H-NMR). The multivariate statistical analysis was conducted by using principal component analysis (PCA), partial least squares-discriminant analysis (PLS-DA) and orthogonal partial least squares-discriminant analysis (OPLS-DA). Results There were 10 potential biomarkers screened. The levels of acetoacetate and acetone were higher, and levels of lactic acid, carnosine, alanine and isoleucine were significantly lower in UC group than those in control group. The levels of glucose, threonine and carnosine were higher, and levels of valine, alanine and glycine were lower in UC patients with large intestinal dampness-heat pattern than those in UC patients with spleen-stomach qi deficiency pattern. Conclusion There are obviously disorder of glycometabolism, amino acid metabolism and lipid metabolism in UC patients with deficiency or excessiveness patterns. The pattern differentiation and classification of UC has a metabolite basis.

Key words: ulcerative colitis, metabolomics, H-nuclear magnetic resonance, large intestinal dampness-heat pattern, spleen-stomach qi deficiency pattern

中图分类号: 

  • R259.746