主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

北京中医药大学学报 ›› 2018, Vol. 41 ›› Issue (10): 821-827.doi: 10.3969/j.issn.1006-2157.2018.10.006

• 科技之窗 • 上一篇    下一篇

参枝苓口服液对APPswe/PSldE9小鼠学习记忆和突触超微结构的影响*

吴艺琼, 陈芳, 盛宁, 任映, 杨金铎, 王蓬文#   

  1. 北京中医药大学东直门医院中医内科学教育部和北京市重点实验室 北京 100700
  • 收稿日期:2018-03-16 出版日期:2018-10-30 发布日期:2018-10-30
  • 通讯作者: 王蓬文,女,博士,教授,博士生导师,主要研究方向:神经变性病的发病机制及中医药防治策略,E-mail:pw_wang@163.com
  • 作者简介:吴艺琼,女,在读硕士生
  • 基金资助:
    国家自然科学基金面上资助项目(No. 81573927),北京市教育委员会科学研究与研究生培养共建资助项目(No.2016,No.2017)

Effects of shenzhiling oral liquid on learning, memory and synaptic structure of APPswe/PSldE9 mice*

Wu Yiqiong, Chen Fang, Sheng Ning, Ren Ying, Yang Jinduo, Wang Pengwen#   

  1. Key Laboratory of Chinese Internal Medicine of Ministry of Education and Beijing, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700,China
  • Received:2018-03-16 Online:2018-10-30 Published:2018-10-30

摘要: 目的 观察参枝苓口服液对双转基因(APPswe/PSldE9)小鼠学习记忆和突触超微结构的影响,探讨其在阿尔茨海默病(AD)早期发挥治疗作用的可能作用机制。方法 将75 只APPswe/PSldE9小鼠随机分为5组:模型组、多奈哌齐组、参枝苓大剂量组、中剂量组和小剂量组,每组15只;同背景 C57/BL6J 小鼠15只作为正常对照组。参枝苓大剂量[50 g/(kg·d)]、中剂量[25 g/(kg·d)]、小剂量[12.5 g/(kg·d)]组,多奈哌齐组[0.92 mg/(kg·d)],分别給予灌胃治疗3个月,正常组和模型组小鼠给予等体积蒸馏水灌胃。治疗3个月后,采用Morris 水迷宫测试评价各组小鼠空间学习记忆能力,运用透射电镜观察海马CA1区突触的超微结构。结果 Morris 水迷宫测试显示模型组与正常组小鼠相比,逃避潜伏期、游泳距离显著延长(P<0.01),穿越平台次数和目标象限停留时间明显减少(P<0.01),治疗后参枝苓中、小剂量组逃避潜伏期、游泳距离明显缩短(P<0.05),穿越平台次数和目标象限停留时间明显增加(P<0.05)。结论 参枝苓能改善APPswe/PSldE9小鼠学习记忆能力,其潜在机制可能与改善小鼠海马CA1区突触结构和数目,进而改善突触可塑性有关。

关键词: 阿尔茨海默病, 水迷宫, 突触, APPswe/PSldE9小鼠

Abstract: Objective To observe the effects of shenzhiling (SZL) oral liquid on learning, memory and synaptic structure of APPswe/PSldE9 mice. Methods Seventy-five APPswe/PSldE9 mice were randomly divided into model group, donepezil group(0.92 mg/(kg·d)), high-dose SZL (50 g/(kg·d)) group, mid-dose SZL (25 g/(kg·d)) group, and low-dose(12.5 g/(kg·d)) SZL groups (n=15 in each group). 15 C57/BL6J mice were used as the normal control group. For the model and normal group, mice were administered oral gavage of distilled water while the interventional groups received corresponding medicine for three months. Learning and memory was evaluated by using Morris water maze test, and the ultrastructure of the synapses were observed with transmission electron microscope. Results Compared with the normal group, mice in the model group had a longer escape latency and swimming distance (P<0.01); reduced times across the platform and stay in the target platform quadrant (P<0.01). After SZL treatment, escape latency was reduced and the swimming distances was shorter (P<0.05); times across the platform and stay in the target platform quadrant were increased, especially in the mid-dose and low-dose groups (P<0.01). The ultrastructure of the synapses was improved in the SZL groups than that in the model group, especially in the mid-dose and lowdose groups. Conclusion SZL oral liquid could improve learning and memory ability, and improve ultrastructure of the synapses in APPswe/PSldE9 mice. This may be one of the important mechanisms of improving cognitive function.

Key words: Alzheimer’s disease, Morris water maze, synapses, APPswe/PSldE9 mice

中图分类号: 

  • R285.5