主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

北京中医药大学学报 ›› 2019, Vol. 42 ›› Issue (1): 21-29.doi: 10.3969/j.issn.1006-2157.2019.01.005

• 中药药理 • 上一篇    下一篇

玄参提取物对甲亢模型大鼠肝脏代谢组学的影响

张宁1,2, 李自辉1, 赵洪伟1, 庞牧1, 叶涛2, 刘树民1#   

  1. 1 黑龙江中医药大学药物安全性评价中心 黑龙江 150040;
    2 贵阳中医学院第一附属医院
  • 收稿日期:2018-07-14 出版日期:2019-01-30 发布日期:2019-03-01
  • 通讯作者: 刘树民,男,博士,教授,研究方向:中药药性理论及药效物质基础研究,E-mail:keji-liu@163.com
  • 作者简介:张宁,女,博士,副主任药师
  • 基金资助:
    国家重点基础研究发展计划(973计划)资助项目(No.2013CB531804),国家自然科学基金青年科学基金资助项目(No.81302898),黑龙江省青年科学基金资助项目(No.QC2012C038),黑龙江省教育厅科学技术研究项目(No.12541744),黑龙江中医药大学科研基金资助项目(No.B201104)

Impact of extracts of Xuanshen on liver metabolome in rat model of hyperthyroidism

Zhang Ning1, 2, Li Zihui1, Zhao Hongwei1, Pang Mu1, Ye Tao2, Liu Shumin1#   

  1. 1 Drug Safety Evaluation Center, Heilongjiang University of Chinese Medicine, Heilongjiang 150040, China;
    2 First Affiliated Hospital of Guiyang University of Chinese Medicine, Guizhou 550001, China
  • Received:2018-07-14 Online:2019-01-30 Published:2019-03-01
  • Contact: Liu Shumin, male, PhD., Professor. Research direction: property theory and pharmacodynamic material basis of Chinese materia medica. E-mail: keji-liu@163.com
  • Supported by:
    National Key Basic Research Program of China (973 Program,No. 2013CB531804), National Natural Science Funds for Young Scholar (No. 81302898), Heilongjiang Province Science Foundation for Youths (No. QC2012C038), Science and Technology foundation of Heilongjiang Province Educational Committee (No. 12541744)

摘要: 目的 探讨玄参提取物对甲亢模型大鼠肝脏代谢组学的作用及作用机制。方法 通过超高效液相色谱飞行时间质谱技术分析优甲乐及玄参提取物作用下的大鼠肝脏代谢物谱。应用正交偏最小二乘判别分析法研究空白组和模型组之间的代谢物谱差异,并通过变量重要性投影(VIP)在肝脏中发现了潜在生物标志物。通过比较潜在生物标志物的含量差异,探讨玄参的干预作用。结果 在模型组大鼠肝脏代谢物中发现了21个潜在生物标志物,而玄参提取物显著回调的代谢物有14个,分别参与糖基磷脂酰肌醇(GPI)锚生物合成、花生四烯酸代谢、嘌呤代谢、α-亚麻酸代谢、亚油酸代谢、谷胱甘肽代谢和甘油磷脂代谢。结论 玄参提取物可能通过改善甲亢模型大鼠肝脏中相关潜在生物标志物表达及其相关代谢通路发挥治疗甲亢的作用。

关键词: 玄参, 优甲乐, 甲状腺功能亢进症, 肝脏代谢组学, 大鼠

Abstract: Objective To investigate the effect and possible mechanism of extracts of Xuanshen (Scrophularia Root, Radix Scrophulariae) on liver metabolome in rat model of hyperthyroidism. Methods The rat liver metabolites spectrums effected by Euthyrox and Xuanshen extracts were analyzed by using ultra high performance liquid chromatography-time of-flight-mass spectrometry (UPLC-TOF-MS). The difference in metabolites spectrums was studied between blank group and model group by using orthogonal partial least squares-discriminant analysis (OPLS-DA). The potential biomarkers were found in liver through variable importance projection (VIP). The interventional effects of Xuanshen extracts were discussed through comparing the difference in content of potential biomarkers. Results There were 21 potential biomarkers found in rat liver in model group, and among them 14 metabolites were significantly called back by Xuanshen extracts. The biosynthesis of glycosylphosphatidylinositol (GPI)-anchor, and metabolisms of arachidonic acid, purine, α-linolenic acid, linoleic acid, glutathione and glycerophospholipid were significantly improved by Xuanshen extracts in Euthyrox-induced hyperthyroidism rat model. Conclusion Xuanshen extracts can play a role of treating hyperthyroidism through improving relevant liver potential biomarkers and metabolism pathways in hyperthyroidism rat model.

Key words: Xuanshen (Scrophularia Root, Radix Scrophulariae), Euthyrox, hyperthyroidism, liver metabonomics, rats

中图分类号: 

  • R285.5