主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

北京中医药大学学报 ›› 2019, Vol. 42 ›› Issue (2): 126-130.doi: 10.3969/j.issn.1006-2157.2019.02.007

• 中药药理 • 上一篇    下一篇

慈姑多糖对异烟肼和利福平联用致损伤小鼠肝细胞凋亡的保护作用

韩悦1,王晶1,张园园2,柯秀慧1,,张聪1,王一辰1李孟慧1,廖艳1#   

  1. 1 北京中医药大学中医学院 北京 100029;
    2 北京中医药大学中药学院
  • 出版日期:2019-02-28 发布日期:2019-02-28
  • 通讯作者: 廖艳,女,博士,副教授,硕士生导师,E-mail:liaoyanaa@sohu.com
  • 作者简介:韩悦,女,在读硕士生
  • 基金资助:
    国家自然科学基金项目(No.81503633),校园食育教育与实践项目(No.1000041510012),北京中医药大学校级科研纵向发展基金项目(No.2018-2XF2JJ-030)

Protective effects of sagittaria sagittifolin polysaccharide on isoniazid/rifampicin-induced apoptosis of mouse liver cells

Han Yue1, Wang Jing1, Zhang Yuanyuan2, Ke Xiuhui1, Zhang Cong1, Wang Yichen1, Li Menghui1, Liao Yan1#   

  1. 1 School of Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China;
    2 School of Chinese Materia Medica, Beijing 102488, China
  • Online:2019-02-28 Published:2019-02-28
  • Contact: A/Liao yan, Ph.D., Master supervisor. School of Chinese Medicine, Beijing University of Chinese Medicine, E-mail: liaoyanaa@sohu.com

摘要: 目的 探讨慈姑多糖对异烟肼和利福平联用致损伤小鼠肝细胞凋亡基因Bax、Bcl-2表达的影响。方法 制造异烟肼(INH)和利福平(RFP)联用小鼠肝损伤模型,HE染色观察肝组织病理变化,筛选慈姑多糖肝损伤体内保护最佳剂量。RT-PCR和Western-blot检测小鼠肝细胞中Bax和Bcl-2的mRNA及蛋白表达。结果 与对照组相比,INH/RFP模型组小鼠肝组织形态明显改变,小鼠肝细胞中Bax、Bcl-2的表达上升,Bcl-2/Bax减小(P<0.05);与模型组相比,慈姑多糖各剂量均能减轻INH/RFP所致小鼠肝组织病变,高剂量组改善最明显,作为最佳剂量进行后续实验;小鼠肝细胞中高剂量慈姑多糖组Bax表达下降,Bcl-2的表达上升,Bcl-2/Bax增大(P<0.05)。结论 慈姑多糖可以对抗INH/RFP联用致肝损伤,可能与抑制Bax的表达并提高Bcl-2的表达从而减少细胞的凋亡有关。

关键词: 慈姑多糖, 肝损伤, 异烟肼/利福平, 细胞凋亡, Bax, Bcl-2, 小鼠

Abstract: Objective To explore the effects of sagittaria sagittifolin polysaccharide (SSP) on the expression of Bax and Bcl-2 in isoniazid (INH)/rifampicin (RfP)-induced hepatic injury liver cells in mice. Methods Models of live injured mice were induced with INH/RFP. Liver tissues pathology was observed by using HE staining to determine the optimal SSP dose. RT-PCR and Western-blot assay were used to measure the levels of mRNA and protein of Bax and Bcl-2. Results Compared with the control group, liver tissues of mice from the model group presented with significant changes. The expression of Bcl-2 significantly increased in mice, and Bcl-2/Bax decreased (P<0.05). Compared with model group, pathological changes of liver tissues were alleviated in all SSP treated groups, especially in high-dose group. Expression of Bax decreased, expression of Bcl-2 significantly increased and Bcl-2/Bax increased in mouse liver cells (P<0.05). Conclusion SSP may protect liver cells against INH/RFP induced apoptosis by inhibiting the expression of Bax as well as increasing the expression of Bcl-2.

Key words: sagittaria sagittifolin polysaccharide, liver injury, INH/RFP, cell apoptosis, Bax, Bcl-2, mice

中图分类号: 

  • R285.5